HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 22, 20405-20412, May 30, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/22/20405    most recent M211795200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, X. Articles by Martinez, E. Search for Related Content PubMed PubMed Citation Articles by Liu, X. Articles by Martinez, E. Social Bookmarking                      What's this?

c-Myc Transformation Domain Recruits the Human STAGA Complex and Requires TRRAP and GCN5 Acetylase Activity for Transcription Activation^*

Xiaohui Liu , Jerusalem Tesfai , Yvonne A. Evrard , Sharon Y. R. Dent  and Ernest Martinez  ¶

From the Department of Biochemistry, University of California, Riverside, California 92521 and the Department of Biochemistry and Molecular Biology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas 77030

Deregulation of the c-Myc oncoprotein (Myc) is implicated in many types of cancer. Myc is a sequence-specific transcription factor that regulates transcription of genes involved in the control of cell proliferation and apoptosis via mechanisms that are still poorly understood. Cell transformation by Myc involves its association with the transformation-transactivation domain-associated protein (TRRAP) and the human histone acetyltransferase (HAT) GCN5. TRRAP and GCN5 are components of a variety of shared and distinct multiprotein HAT complexes with diverse functions. Myc induces TRRAP recruitment and histone hyperacetylation at specific Myc-activated genes in vivo. However, the identity of the HAT complexes recruited by Myc and the roles of TRRAP and GCN5 in Myc function are still unclear. Here we show that Myc co-recruits TRRAP and GCN5 via direct physical interactions of its N-terminal activation/transformation domain with the human STAGA (SPT3-TAF-GCN5 acetylase) coactivator complex. We demonstrate that GCN5 and TRRAP cooperate to enhance transcription activation by the N-terminal activation domain of Myc in vivo and that this synergy requires both the SPT3/GCN5 interaction domain of TRRAP and the HAT activity of GCN5. Thus, TRRAP might function as an adaptor within the STAGA complex, which helps recruit GCN5 HAT activity to Myc during transcription activation.

Received for publication, November 19, 2002 , and in revised form, March 10, 2003.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Biochemistry, University of California, Riverside, CA 92521. Tel.: 909-787-2031; Fax: 909-787-4434; E-mail: ernest.martinez{at}ucr.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. M. Gamper and R. G. Roeder Multivalent Binding of p53 to the STAGA Complex Mediates Coactivator Recruitment after UV Damage Mol. Cell. Biol., April 15, 2008; 28(8): 2517 - 2527. [Abstract] [Full Text] [PDF]

				P. Sekaric, J. J. Cherry, and E. J. Androphy Binding of Human Papillomavirus Type 16 E6 to E6AP Is Not Required for Activation of hTERT J. Virol., January 1, 2008; 82(1): 71 - 76. [Abstract] [Full Text] [PDF]

				X. Liu, M. Vorontchikhina, Y.-L. Wang, F. Faiola, and E. Martinez STAGA Recruits Mediator to the MYC Oncoprotein To Stimulate Transcription and Cell Proliferation Mol. Cell. Biol., January 1, 2008; 28(1): 108 - 121. [Abstract] [Full Text] [PDF]

				G.-H. Peng and S. Chen Crx activates opsin transcription by recruiting HAT-containing co-activators and promoting histone acetylation Hum. Mol. Genet., October 15, 2007; 16(20): 2433 - 2452. [Abstract] [Full Text] [PDF]

				F. Robert, S. Hardy, Z. Nagy, C. Baldeyron, R. Murr, U. Dery, J.-Y. Masson, D. Papadopoulo, Z. Herceg, and L. Tora The Transcriptional Histone Acetyltransferase Cofactor TRRAP Associates with the MRN Repair Complex and Plays a Role in DNA Double-Strand Break Repair Mol. Cell. Biol., January 15, 2006; 26(2): 402 - 412. [Abstract] [Full Text] [PDF]

				F. Faiola, X. Liu, S. Lo, S. Pan, K. Zhang, E. Lymar, A. Farina, and E. Martinez Dual Regulation of c-Myc by p300 via Acetylation-Dependent Control of Myc Protein Turnover and Coactivation of Myc-Induced Transcription Mol. Cell. Biol., December 1, 2005; 25(23): 10220 - 10234. [Abstract] [Full Text] [PDF]

				H. Szutorisz, C. Canzonetta, A. Georgiou, C.-M. Chow, L. Tora, and N. Dillon Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage Mol. Cell. Biol., March 1, 2005; 25(5): 1804 - 1820. [Abstract] [Full Text] [PDF]