HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 24, 21474-21482, June 13, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/24/21474    most recent M212098200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, X.-L. Articles by Simmen, R. C. M. Search for Related Content PubMed PubMed Citation Articles by Zhang, X.-L. Articles by Simmen, R. C. M. Social Bookmarking                      What's this?

Selective Interactions of Krüppel-like Factor 9/Basic Transcription Element-binding Protein with Progesterone Receptor Isoforms A and B Determine Transcriptional Activity of Progesterone-responsive Genes in Endometrial Epithelial Cells^*

Xue-Lian Zhang , Daying Zhang , Frank J. Michel , Jason L. Blum , Frank A. Simmen   and Rosalia C. M. Simmen   ¶

From the Interdisciplinary Concentration in Animal Molecular and Cell Biology, University of Florida, Gainesville, Florida 32611-0910, Arkansas Children's Nutrition Center and the Department of Physiology & Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72202

The Sp/KLF transcription factor basic transcription element-binding protein (BTEB1) regulates gene transcription by binding to GC-rich sequence motifs present in the promoters of numerous tissue-specific as well as housekeeping genes. Similar to other members of this family, BTEB1 can act as a transactivator or transrepressor depending on cell and promoter context, although the molecular mechanism underlying these distinct activities remains unclear. Here we report that BTEB1 can mediate signaling pathways involving the nuclear receptor for the steroid hormone progesterone in endometrial epithelial cells by its selective interaction with the progesterone receptor (PR) isoforms, PR-A and PR-B. Functional interaction with ligand-activated PR-B resulted in superactivation of PR-B transactivity, facilitated the recruitment of the transcriptional integrator CREB-binding protein within the PR-dimer, and was dependent on the structure of the ligand bound by PR-B. By contrast, BTEB1 did not influence agonist-bound PR-A transactivity, although it augmented PR-A inhibition of PR-B-mediated transactivation as well as potentiated ligand-independent PR-A transcriptional activity in the presence of CREB-binding protein. We also demonstrate similar positive modulatory actions of BTEB1-related family members Krüppel-like family (KLF) 13/FKLF2/BTEB3 and Sp1 on PR-B transactivity. Further, we provide support for the potential significance of the selective functional interactions of PR isoforms with BTEB1 in the peri-implantation uterus using mouse and pig models and in the breast cancer cell lines MCF-7 and T47D. Our results suggest a novel mechanism for the divergent physiological consequences of PR-A and PR-B on progesterone-dependent gene transcription in the uterus involving select KLF members.

Received for publication, November 27, 2002 , and in revised form, March 10, 2003.

^* This work was supported in part by the National Institutes of Health Grant HD21961 and USDA-CRIS-6251-5100-002-06S. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Tel.: 501-364-2849; Fax: 501-364-3161; E-mail: simmenrosalia{at}uams.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. Zeng, M. C. Velarde, F. A. Simmen, and R. C.M. Simmen Delayed Parturition and Altered Myometrial Progesterone Receptor Isoform A Expression in Mice Null for Kruppel-Like Factor 9 Biol Reprod, June 1, 2008; 78(6): 1029 - 1037. [Abstract] [Full Text] [PDF]

				S. Natesampillai, J. Kerkvliet, P. C. K. Leung, and J. D. Veldhuis Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E385 - E391. [Abstract] [Full Text] [PDF]

				M. C. Velarde, Z. Zeng, J. R. McQuown, F. A. Simmen, and R. C. M. Simmen Kruppel-Like Factor 9 Is a Negative Regulator of Ligand-Dependent Estrogen Receptor {alpha} Signaling in Ishikawa Endometrial Adenocarcinoma Cells Mol. Endocrinol., December 1, 2007; 21(12): 2988 - 3001. [Abstract] [Full Text] [PDF]

				F. A. Simmen, R. Xiao, M. C. Velarde, R. D. Nicholson, M. T. Bowman, Y. Fujii-Kuriyama, S. P. Oh, and R. C. M. Simmen Dysregulation of intestinal crypt cell proliferation and villus cell migration in mice lacking Kruppel-like factor 9 Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1757 - G1769. [Abstract] [Full Text] [PDF]

				X. Xu, J. Ding, G. Rao, J. Shen, R. A. Prinz, N. Rana, and W.P. Dmowski Estradiol induces heparanase-1 expression and heparan sulphate proteoglycan degradation in human endometrium Hum. Reprod., April 1, 2007; 22(4): 927 - 937. [Abstract] [Full Text] [PDF]

				H. Pan, L. Zhu, Y. Deng, and J. W. Pollard Microarray Analysis of Uterine Epithelial Gene Expression during the Implantation Window in the Mouse Endocrinology, October 1, 2006; 147(10): 4904 - 4916. [Abstract] [Full Text] [PDF]

				M. C. Velarde, M. Iruthayanathan, R. R. Eason, D. Zhang, F. A. Simmen, and R. C. M. Simmen Progesterone Receptor Transactivation of the Secretory Leukocyte Protease Inhibitor Gene in Ishikawa Endometrial Epithelial Cells Involves Recruitment of Kruppel-Like Factor 9/Basic Transcription Element Binding Protein-1 Endocrinology, April 1, 2006; 147(4): 1969 - 1978. [Abstract] [Full Text] [PDF]

				S. Natesampillai, M. E. Fernandez-Zapico, R. Urrutia, and J. D. Veldhuis A Novel Functional Interaction between the Sp1-like Protein KLF13 and SREBP-Sp1 Activation Complex Underlies Regulation of Low Density Lipoprotein Receptor Promoter Function J. Biol. Chem., February 10, 2006; 281(6): 3040 - 3047. [Abstract] [Full Text] [PDF]

				J. D. Graham, M. L. Yager, H. D. Hill, K. Byth, G. M. O'Neill, and C. L. Clarke Altered Progesterone Receptor Isoform Expression Remodels Progestin Responsiveness of Breast Cancer Cells Mol. Endocrinol., November 1, 2005; 19(11): 2713 - 2735. [Abstract] [Full Text] [PDF]

				M. C. Velarde, Y. Geng, R. R. Eason, F. A. Simmen, and R. C.M. Simmen Null Mutation of Kruppel-Like Factor9/Basic Transcription Element Binding Protein-1 Alters Peri-Implantation Uterine Development in Mice Biol Reprod, September 1, 2005; 73(3): 472 - 481. [Abstract] [Full Text] [PDF]

				S. Cao, M. E. Fernandez-Zapico, D. Jin, V. Puri, T. A. Cook, L. O. Lerman, X.-Y. Zhu, R. Urrutia, and V. Shah KLF11-mediated Repression Antagonizes Sp1/Sterol-responsive Element-binding protein-induced Transcriptional Activation of Caveolin-1 in Response to Cholesterol Signaling J. Biol. Chem., January 21, 2005; 280(3): 1901 - 1910. [Abstract] [Full Text] [PDF]

				S. Amini, M. Saunders, K. Kelley, K. Khalili, and B. E. Sawaya Interplay between HIV-1 Vpr and Sp1 Modulates p21WAF1 Gene Expression in Human Astrocytes J. Biol. Chem., October 29, 2004; 279(44): 46046 - 46056. [Abstract] [Full Text] [PDF]

				R. C. M. Simmen, R. R. Eason, J. R. McQuown, A. L. Linz, T.-J. Kang, L. Chatman Jr., S. R. Till, Y. Fujii-Kuriyama, F. A. Simmen, and S. P. Oh Subfertility, Uterine Hypoplasia, and Partial Progesterone Resistance in Mice Lacking the Kruppel-like Factor 9/Basic Transcription Element-binding Protein-1 (Bteb1) Gene J. Biol. Chem., July 9, 2004; 279(28): 29286 - 29294. [Abstract] [Full Text] [PDF]

				J. Fahnenstich, A. Nandy, K. Milde-Langosch, T. Schneider-Merck, N. Walther, and B. Gellersen Promyelocytic leukaemia zinc finger protein (PLZF) is a glucocorticoid- and progesterone-induced transcription factor in human endometrial stromal cells and myometrial smooth muscle cells Mol. Hum. Reprod., October 1, 2003; 9(10): 611 - 623. [Abstract] [Full Text] [PDF]