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J. Biol. Chem., Vol. 278, Issue 25, 22193-22198, June 20, 2003

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Effects of Phospholipid Composition on MinD-Membrane Interactions in Vitro and in Vivo^*

Eugenia Mileykovskaya , Itzhak Fishov  ¶, Xueyao Fu , Brian D. Corbin ||, William Margolin || and William Dowhan  **

From the Departments of Biochemistry and Molecular Biology and ^||Microbiology and Molecular Genetics, The University of Texas Medical School, Houston, Texas 77030 and the Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel

The peripheral membrane ATPase MinD is a component of the Min system responsible for correct placement of the division site in Escherichia coli cells. By rapidly migrating from one cell pole to the other, MinD helps to block unwanted septation events at the poles. MinD is an amphitropic protein that is localized to the membrane in its ATP-bound form. A C-terminal domain essential for membrane localization is predicted to be an amphipathic -helix with hydrophobic residues interacting with lipid acyl chains and cationic residues on the opposite face of the helix interacting with the head groups of anionic phospholipids (Szeto, T. H., Rowland, S. L., Rothfield, L. I., and King, G. F. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 15693–15698). To investigate whether E. coli MinD displays a preference for anionic phospholipids, we first examined the localization dynamics of a green fluorescent protein-tagged derivative of MinD expressed in a mutant of E. coli that lacks phosphatidylethanolamine. In these cells, which contain only anionic phospholipids (phosphatidylglycerol and cardiolipin), green fluorescent protein-MinD assembled into dynamic focal clusters instead of the broad zones typical of cells with normal phospholipid content. In experiments with liposomes composed of only zwitterionic, only anionic, or a mixture of anionic and zwitterionic phospholipids, purified MinD bound to these liposomes in the presence of ATP with positive cooperativity with respect to the protein concentration and exhibited Hill coefficients of about 2. Oligomerization of MinD on the liposome surface also was detected by fluorescence resonance energy transfer between MinD molecules labeled with different fluorescent probes. The affinity of MinD-ATP for anionic liposomes as well as liposomes composed of both anionic and zwitterionic phospholipids increased 9- and 2-fold, respectively, relative to zwitterionic liposomes. The degree of acyl chain unsaturation contributed positively to binding strength. These results suggest that MinD has a preference for anionic phospholipids and that MinD oscillation behavior, and therefore cell division site selection, may be regulated by membrane phospholipid composition.

Received for publication, March 13, 2003 , and in revised form, March 31, 2003.

^* This work was supported in part by National Institutes of Health Grants GM20487 (to W. D.) and GM61074 (to W. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Supported in part by The University of Texas Graduate School of Biomedical Sciences.

^** To whom correspondence should be addressed: P. O. Box 20708, Dept. of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, TX 77225. Tel.: 713-500-6051; Fax: 713-500-0652; E-mail: William.Dowhan{at}uth.tmc.edu.

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