|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 26, 23656-23665, June 27, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ADAMTS1/METH1 Inhibits Endothelial Cell Proliferation by Direct Binding and Sequestration of VEGF165*
From the Department of Molecular, Cell, and Developmental Biology, Molecular Biology Institute and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California 90095
ADAMTS1 is a metalloprotease previously shown to inhibit angiogenesis in a variety of in vitro and in vivo assays. In the present study, we demonstrate that ADAMTS1 significantly blocks VEGFR2 phosphorylation with consequent suppression of endothelial cell proliferation. The effect on VEGFR2 function was due to direct binding and sequestration of VEGF165 by ADAMTS1. Binding was confirmed by co-immunoprecipitation and cross-linking analysis. Inhibition of VEGF function was reversible, as active VEGF could be recovered from the complex. The interaction required the heparin-binding domain of the growth factor, because VEGF121 failed to bind to ADAMTS1. Structure/function analysis with independent ADAMTS1 domains indicated that binding to VEGF165 was mediated by the carboxyl-terminal (CT) region. ADAMTS1 and VEGF165 were also found in association in tumor extracts. These findings provide a mechanism for the anti-angiogenic activity of ADAMTS1 and describe a novel modulator of VEGF bioavailability.
Received for publication, December 19, 2002 , and in revised form, April 21, 2003.
* This work was supported by Grant NIH/RO1CA77420 from the National Institutes of Health (to M. L. I. A). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Recipient of a postdoctoral fellowship from the Department of Defense (DOD)
Congressionally Directed Medical Research Programs (CDMRP) (DOD Breast Cancer
Research Program, DAMD17-02-1-0329).
Recipient of a scholarship from the Giannini Family Foundation.
¶ To whom correspondence should be addressed: Molecular Biology Institute, 611 Charles Young Drive East, Los Angeles, CA 90095. Tel.: 310-794-5763; Fax: 310-794-5766; E-mail: arispe{at}mbi.ucla.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T.-K. Ito, G. Ishii, S. Saito, K. Yano, A. Hoshino, T. Suzuki, and A. Ochiai Degradation of soluble VEGF receptor-1 by MMP-7 allows VEGF access to endothelial cells Blood, March 5, 2009; 113(10): 2363 - 2369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Rocks, G. Paulissen, F. Quesada-Calvo, C. Munaut, M.-L. A. Gonzalez, M. Gueders, J. Hacha, C. Gilles, J.-M. Foidart, A. Noel, et al. ADAMTS-1 Metalloproteinase Promotes Tumor Development through the Induction of a Stromal Reaction In vivo Cancer Res., November 15, 2008; 68(22): 9541 - 9550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Su, E. A. Mendoza, H.-i. Kwak, and K. J. Bayless Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1215 - C1229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Lijnen Angiogenesis and obesity Cardiovasc Res, May 1, 2008; 78(2): 286 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Basile, K. Fredrich, B. Chelladurai, E. C. Leonard, and A. R. Parrish Renal ischemia reperfusion inhibits VEGF expression and induces ADAMTS-1, a novel VEGF inhibitor Am J Physiol Renal Physiol, April 1, 2008; 294(4): F928 - F936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Dunning, M. Lane, H. M. Brown, C. Yeo, R. L. Robker, and D. L. Russell Altered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes Hum. Reprod., November 1, 2007; 22(11): 2842 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Llamazares, A. J. Obaya, A. Moncada-Pazos, R. Heljasvaara, J. Espada, C. Lopez-Otin, and S. Cal The ADAMTS12 metalloproteinase exhibits anti-tumorigenic properties through modulation of the Ras-dependent ERK signalling pathway J. Cell Sci., October 15, 2007; 120(20): 3544 - 3552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ren, P. C. Charles, C. Zhang, Y. Wu, H. Wang, and C. Patterson Gene expression profiles identify a role for cyclooxygenase 2-dependent prostanoid generation in BMP6-induced angiogenic responses Blood, April 1, 2007; 109(7): 2847 - 2853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xu, Y. Yu, and E. J. Duh Vascular Endothelial Growth Factor Upregulates Expression of ADAMTS1 in Endothelial Cells through Protein Kinase C Signaling. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 4059 - 4066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. X. Torres-Collado, W. Kisiel, M. L. Iruela-Arispe, and J. C. Rodriguez-Manzaneque ADAMTS1 Interacts with, Cleaves, and Modifies the Extracellular Location of the Matrix Inhibitor Tissue Factor Pathway Inhibitor-2 J. Biol. Chem., June 30, 2006; 281(26): 17827 - 17837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Lee, M. D. Canny, A. De Erkenez, D. Krilleke, Y.-S. Ng, D. T. Shima, A. Pardi, and F. Jucker A therapeutic aptamer inhibits angiogenesis by specifically targeting the heparin binding domain of VEGF165 PNAS, December 27, 2005; 102(52): 18902 - 18907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Shozu, N Minami, H Yokoyama, M Inoue, H Kurihara, K Matsushima, and K Kuno ADAMTS-1 is involved in normal follicular development, ovulatory process and organization of the medullary vascular network in the ovary J. Mol. Endocrinol., October 1, 2005; 35(2): 343 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Jazaeri, C. S. Awtrey, G. V.R. Chandramouli, Y. E. Chuang, J. Khan, C. Sotiriou, O. Aprelikova, C. J. Yee, K. K. Zorn, M. J. Birrer, et al. Gene Expression Profiles Associated with Response to Chemotherapy in Epithelial Ovarian Cancers Clin. Cancer Res., September 1, 2005; 11(17): 6300 - 6310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Krampert, S. Kuenzle, S. N.-M. Thai, N. Lee, M. L. Iruela-Arispe, and S. Werner ADAMTS1 Proteinase Is Up-regulated in Wounded Skin and Regulates Migration of Fibroblasts and Endothelial Cells J. Biol. Chem., June 24, 2005; 280(25): 23844 - 23852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, S. M. Jilani, G. V. Nikolova, D. Carpizo, and M. L. Iruela-Arispe Processing of VEGF-A by matrix metalloproteinases regulates bioavailability and vascular patterning in tumors J. Cell Biol., May 23, 2005; 169(4): 681 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G D Perkins, J Roberts, D F McAuley, L Armstrong, A Millar, F Gao, and D R Thickett Regulation of vascular endothelial growth factor bioactivity in patients with acute lung injury Thorax, February 1, 2005; 60(2): 153 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Wight The ADAMTS Proteases, Extracellular Matrix, and Vascular Disease: Waking the Sleeping Giant(s)! Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 12 - 14. [Full Text] [PDF]
|
|
|
|
 |
|
 G Eisenhofer, T-T Huynh, K Pacak, F M Brouwers, M M Walther, W M Linehan, P J Munson, M Mannelli, D S Goldstein, and A G Elkahloun Distinct gene expression profiles in norepinephrine- and epinephrine-producing hereditary and sporadic pheochromocytomas: activation of hypoxia-driven angiogenic pathways in von Hippel-Lindau syndrome Endocr. Relat. Cancer, December 1, 2004; 11(4): 897 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Minami, K. Horiuchi, M. Miura, Md. R. Abid, W. Takabe, N. Noguchi, T. Kohro, X. Ge, H. Aburatani, T. Hamakubo, et al. Vascular Endothelial Growth Factor- and Thrombin-induced Termination Factor, Down Syndrome Critical Region-1, Attenuates Endothelial Cell Proliferation and Angiogenesis J. Biol. Chem., November 26, 2004; 279(48): 50537 - 50554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nakamura, S. Hirohata, T. Murakami, T. Miyoshi, K. Demircan, T. Oohashi, H. Ogawa, K. Koten, K. Toeda, S. Kusachi, et al. Dynamic Induction of ADAMTS1 Gene in the Early Phase of Acute Myocardial Infarction J. Biochem., October 1, 2004; 136(4): 439 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. T. Somerville, J.-M. Longpre, E. D. Apel, R. M. Lewis, L. W. Wang, J. R. Sanes, R. Leduc, and S. S. Apte ADAMTS7B, the Full-length Product of the ADAMTS7 Gene, Is a Chondroitin Sulfate Proteoglycan Containing a Mucin Domain J. Biol. Chem., August 20, 2004; 279(34): 35159 - 35175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Longpre and R. Leduc Identification of Prodomain Determinants Involved in ADAMTS-1 Biosynthesis J. Biol. Chem., August 6, 2004; 279(32): 33237 - 33245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Young, B. Tumlinson, and R. L. Stouffer ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum: divergent patterns and stage-dependent regulation during the natural menstrual cycle Mol. Hum. Reprod., August 1, 2004; 10(8): 559 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Minami, A. Sugiyama, S.-Q. Wu, R. Abid, T. Kodama, and W. C. Aird Thrombin and Phenotypic Modulation of the Endothelium Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 41 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zheng, K. Nishio, E. M. Majerus, and J. E. Sadler Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13 J. Biol. Chem., August 8, 2003; 278(32): 30136 - 30141. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |