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J. Biol. Chem., Vol. 278, Issue 27, 24825-24830, July 4, 2003

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Crystal Structure of Neisserial Surface Protein A (NspA), a Conserved Outer Membrane Protein with Vaccine Potential^*

Lucy Vandeputte-Rutten , Martine P. Bos  ¶, Jan Tommassen  and Piet Gros  ||

From the Department of Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research and the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

The neisserial surface protein A (NspA) from Neisseria meningitidis is a promising vaccine candidate because it is highly conserved among meningococcal strains and induces bactericidal antibodies. NspA is a homolog of the Opa proteins, which mediate adhesion to host cells. Here, we present the crystal structure of NspA, determined to 2.55-Å resolution. NspA forms an eight-stranded antiparallel -barrel. The four loops at the extracellular side of the NspA molecule form a long cleft, which contains mainly hydrophobic residues and harbors a detergent molecule, suggesting that the protein might function in the binding of hydrophobic ligands, such as lipids. In addition, the structure provides a starting point for structure-based vaccine design.

Received for publication, March 19, 2003 , and in revised form, April 24, 2003.

The atomic coordinates and structure factors (code 1P4T) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).

^* This work was supported by the council for Chemical Sciences of the Netherlands Organization for Scientific Research (NWO-CW). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Supported by the European Community project MenB vaccine QLRT-1999-CT-00359.

^|| To whom correspondence should be addressed. Tel.: 31-30-2533502; Fax: 31-30-2533940; E-mail: p.gros{at}chem.uu.nl.

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