HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 27, 24874-24880, July 4, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/27/24874    most recent M303908200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sreenath, T. Articles by Kulkarni, A. B. Search for Related Content PubMed PubMed Citation Articles by Sreenath, T. Articles by Kulkarni, A. B. Social Bookmarking                      What's this?

Dentin Sialophosphoprotein Knockout Mouse Teeth Display Widened Predentin Zone and Develop Defective Dentin Mineralization Similar to Human Dentinogenesis Imperfecta Type III^*

Taduru Sreenath  , Tamizchelvi Thyagarajan , Bradford Hall , Glenn Longenecker , Rena D'Souza ¶, Sung Hong ¶, J. Tim Wright ||, Mary MacDougall **, John Sauk  and Ashok B. Kulkarni  ¶¶

From the Functional Genomics Unit and Gene Targeting Facility, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, ^¶University of Texas Health Science Center at Houston, Houston, Texas 77030, ^||University of North Carolina, Chapel Hill, North Carolina 27599, ^**University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, and University of Maryland at Baltimore, Baltimore, Maryland 21201

Dentin sialophosphoprotein (Dspp) is mainly expressed in teeth by the odontoblasts and preameloblasts. The Dspp mRNA is translated into a single protein, Dspp, and cleaved into two peptides, dentin sialoprotein and dentin phosphoprotein, that are localized within the dentin matrix. Recently, mutations in this gene were identified in human dentinogenesis imperfecta II (Online Mendelian Inheritance in Man (OMIM) accession number 125490) and in dentin dysplasia II (OMIM accession number 125420) syndromes. Herein, we report the generation of Dspp-null mice that develop tooth defects similar to human dentinogenesis imperfecta III with enlarged pulp chambers, increased width of predentin zone, hypomineralization, and pulp exposure. Electron microscopy revealed an irregular mineralization front and a lack of calcospherites coalescence in the dentin. Interestingly, the levels of biglycan and decorin, small leucine-rich proteoglycans, were increased in the widened predentin zone and in void spaces among the calcospherites in the dentin of null teeth. These enhanced levels correlate well with the defective regions in mineralization and further indicate that these molecules may adversely affect the dentin mineralization process by interfering with coalescence of calcospherites. Overall, our results identify a crucial role for Dspp in orchestrating the events essential during dentin mineralization, including potential regulation of proteoglycan levels.

Received for publication, April 14, 2003

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence may be addressed: 30 Convent Dr., Room 527, Bethesda, MD 20892. Tel.: 301-402-5061; Fax: 301-435-2888; E-mail: tsreenat{at}mail.nih.gov.

^¶¶ To whom correspondence may be addressed: 30 Convent Dr., Room 527, Bethesda, MD 20892. Tel.: 301-435-2887; Fax: 301-435-2888; E-mail: akulkarn{at}mail.nih.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Hao, A. Ramachandran, and A. George Temporal and Spatial Localization of the Dentin Matrix Proteins During Dentin Biomineralization J. Histochem. Cytochem., March 1, 2009; 57(3): 227 - 237. [Abstract] [Full Text] [PDF]

				D.A. McKnight, J.P. Simmer, P.S. Hart, T.C. Hart, and L.W. Fisher Overlapping DSPP Mutations Cause Dentin Dysplasia and Dentinogenesis Imperfecta Journal of Dental Research, December 1, 2008; 87(12): 1108 - 1111. [Abstract] [Full Text] [PDF]

				N.R. Kaipatur, M. Murshed, and M.D. McKee Matrix Gla Protein Inhibition of Tooth Mineralization Journal of Dental Research, September 1, 2008; 87(9): 839 - 844. [Abstract] [Full Text] [PDF]

				S. Chen, J. Gluhak-Heinrich, M. Martinez, T. Li, Y. Wu, H.-H. Chuang, L. Chen, J. Dong, I. Gay, and M. MacDougall Bone Morphogenetic Protein 2 Mediates Dentin Sialophosphoprotein Expression and Odontoblast Differentiation via NF-Y Signaling J. Biol. Chem., July 11, 2008; 283(28): 19359 - 19370. [Abstract] [Full Text] [PDF]

				L. Ye, S. Zhang, H. Ke, L. F. Bonewald, and JQ. Feng Periodontal Breakdown in the Dmp1 Null Mouse Model of Hypophosphatemic Rickets Journal of Dental Research, July 1, 2008; 87(7): 624 - 629. [Abstract] [Full Text] [PDF]

				K. Kawasaki and K.M. Weiss SCPP Gene Evolution and the Dental Mineralization Continuum Journal of Dental Research, June 1, 2008; 87(6): 520 - 531. [Abstract] [Full Text] [PDF]

				Y. Yamakoshi, Y. Lu, J. C.-C. Hu, J.-W. Kim, T. Iwata, K. Kobayashi, T. Nagano, F. Yamakoshi, Y. Hu, M. Fukae, et al. Porcine Dentin Sialophosphoprotein: LENGTH POLYMORPHISMS, GLYCOSYLATION, PHOSPHORYLATION, AND STABILITY J. Biol. Chem., May 23, 2008; 283(21): 14835 - 14844. [Abstract] [Full Text] [PDF]

				L. Malaval, N. M. Wade-Gueye, M. Boudiffa, J. Fei, R. Zirngibl, F. Chen, N. Laroche, J.-P. Roux, B. Burt-Pichat, F. Duboeuf, et al. Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis J. Exp. Med., May 12, 2008; 205(5): 1145 - 1153. [Abstract] [Full Text] [PDF]

				C. Qin, R. D'Souza, and J.Q. Feng Dentin Matrix Protein 1 (DMP1): New and Important Roles for Biomineralization and Phosphate Homeostasis Journal of Dental Research, December 1, 2007; 86(12): 1134 - 1141. [Abstract] [Full Text] [PDF]

				V. Godovikova and H. H. Ritchie Dynamic Processing of Recombinant Dentin Sialoprotein-Phosphophoryn Protein J. Biol. Chem., October 26, 2007; 282(43): 31341 - 31348. [Abstract] [Full Text] [PDF]

				S. de Vega, T. Iwamoto, T. Nakamura, K. Hozumi, D. A. McKnight, L. W. Fisher, S. Fukumoto, and Y. Yamada TM14 Is a New Member of the Fibulin Family (Fibulin-7) That Interacts with Extracellular Matrix Molecules and Is Active for Cell Binding J. Biol. Chem., October 19, 2007; 282(42): 30878 - 30888. [Abstract] [Full Text] [PDF]

				J.-W. Kim and J.P. Simmer Hereditary Dentin Defects Journal of Dental Research, May 1, 2007; 86(5): 392 - 399. [Abstract] [Full Text] [PDF]

				Y. Lu, Y. Xie, S. Zhang, V. Dusevich, L.F. Bonewald, and J.Q. Feng DMP1-targeted Cre Expression in Odontoblasts and Osteocytes Journal of Dental Research, April 1, 2007; 86(4): 320 - 325. [Abstract] [Full Text] [PDF]

				Y. Yamakoshi, J. C-C. Hu, T. Iwata, K. Kobayashi, M. Fukae, and J. P. Simmer Dentin Sialophosphoprotein Is Processed by MMP-2 and MMP-20 in Vitro and in Vivo J. Biol. Chem., December 15, 2006; 281(50): 38235 - 38243. [Abstract] [Full Text] [PDF]

				K. Narayanan, S. Gajjeraman, A. Ramachandran, J. Hao, and A. George Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene Transcription during Early Odontoblast Differentiation J. Biol. Chem., July 14, 2006; 281(28): 19064 - 19071. [Abstract] [Full Text] [PDF]

				S. H. Durand, V. Flacher, A. Romeas, F. Carrouel, E. Colomb, C. Vincent, H. Magloire, M.-L. Couble, F. Bleicher, M.-J. Staquet, et al. Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts. J. Immunol., March 1, 2006; 176(5): 2880 - 2887. [Abstract] [Full Text] [PDF]

				M. L. Paine, W. Luo, H.-J. Wang, P. Bringas Jr., A. Y. W. Ngan, V. G. Miklus, D.-H. Zhu, M. MacDougall, S. N. White, and M. L. Snead Dentin Sialoprotein and Dentin Phosphoprotein Overexpression during Amelogenesis J. Biol. Chem., September 9, 2005; 280(36): 31991 - 31998. [Abstract] [Full Text] [PDF]

				L. Ye, Y. Mishina, D. Chen, H. Huang, S. L. Dallas, M. R. Dallas, P. Sivakumar, T. Kunieda, T. W. Tsutsui, A. Boskey, et al. Dmp1-deficient Mice Display Severe Defects in Cartilage Formation Responsible for a Chondrodysplasia-like Phenotype J. Biol. Chem., February 18, 2005; 280(7): 6197 - 6203. [Abstract] [Full Text] [PDF]

				Y. Yamakoshi, J. C-C. Hu, M. Fukae, T. Iwata, J.-W. Kim, H. Zhang, and J. P. Simmer Porcine Dentin Sialoprotein Is a Proteoglycan with Glycosaminoglycan Chains Containing Chondroitin 6-Sulfate J. Biol. Chem., January 14, 2005; 280(2): 1552 - 1560. [Abstract] [Full Text] [PDF]

				K. Narayanan, A. Ramachandran, M. C. Peterson, J. Hao, A.-B. Kolsto, A. D. Friedman, and A. George The CCAAT Enhancer-binding Protein (C/EBP){beta} and Nrf1 Interact to Regulate Dentin Sialophosphoprotein (DSPP) Gene Expression during Odontoblast Differentiation J. Biol. Chem., October 29, 2004; 279(44): 45423 - 45432. [Abstract] [Full Text] [PDF]

				S. Chen, A. Unterbrink, S. Kadapakkam, J. Dong, T. T. Gu, J. Dickson, H.-H. Chuang, and M. MacDougall Regulation of the Cell Type-specific Dentin Sialophosphoprotein Gene Expression in Mouse Odontoblasts by a Novel Transcription Repressor and an Activator CCAAT-binding Factor J. Biol. Chem., October 1, 2004; 279(40): 42182 - 42191. [Abstract] [Full Text] [PDF]

				C. Qin, O. Baba, and W.T. Butler POST-TRANSLATIONAL MODIFICATIONS OF SIBLING PROTEINS AND THEIR ROLES IN OSTEOGENESIS AND DENTINOGENESIS Critical Reviews in Oral Biology & Medicine, May 1, 2004; 15(3): 126 - 136. [Abstract] [Full Text] [PDF]

				P. H. Tartaix, M. Doulaverakis, A. George, L. W. Fisher, W. T. Butler, C. Qin, E. Salih, M. Tan, Y. Fujimoto, L. Spevak, et al. In Vitro Effects of Dentin Matrix Protein-1 on Hydroxyapatite Formation Provide Insights into in Vivo Functions J. Biol. Chem., April 30, 2004; 279(18): 18115 - 18120. [Abstract] [Full Text] [PDF]

				L. Ye, M. MacDougall, S. Zhang, Y. Xie, J. Zhang, Z. Li, Y. Lu, Y. Mishina, and J. Q. Feng Deletion of Dentin Matrix Protein-1 Leads to a Partial Failure of Maturation of Predentin into Dentin, Hypomineralization, and Expanded Cavities of Pulp and Root Canal during Postnatal Tooth Development J. Biol. Chem., April 30, 2004; 279(18): 19141 - 19148. [Abstract] [Full Text] [PDF]

				G. He and A. George Dentin Matrix Protein 1 Immobilized on Type I Collagen Fibrils Facilitates Apatite Deposition in Vitro J. Biol. Chem., March 19, 2004; 279(12): 11649 - 11656. [Abstract] [Full Text] [PDF]

				K. Yuasa, S. Fukumoto, Y. Kamasaki, A. Yamada, E. Fukumoto, K. Kanaoka, K. Saito, H. Harada, E. Arikawa-Hirasawa, Y. Miyagoe-Suzuki, et al. Laminin {alpha}2 Is Essential for Odontoblast Differentiation Regulating Dentin Sialoprotein Expression J. Biol. Chem., March 12, 2004; 279(11): 10286 - 10292. [Abstract] [Full Text] [PDF]

				B. M. Steiglitz, M. Ayala, K. Narayanan, A. George, and D. S. Greenspan Bone Morphogenetic Protein-1/Tolloid-like Proteinases Process Dentin Matrix Protein-1 J. Biol. Chem., January 9, 2004; 279(2): 980 - 986. [Abstract] [Full Text] [PDF]