| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 28, 25671-25677, July 11, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
Plexins are receptors for the axon guidance molecule semaphorins, and several lines of evidence suggest that Rho family small GTPases are implicated in the downstream signaling of Plexins. Recent studies have demonstrated that Plexin-B1 activates RhoA and induces growth cone collapse through Rho-specific guanine nucleotide exchange factor PDZ-RhoGEF. Here we show that Rnd1, a member of Rho family GTPases, directly interacted with the cytoplasmic domain of Plexin-B1. In COS-7 cells, coexpression of Rnd1 and Plexin-B1 induced cell contraction in response to semaphorin 4D (Sema4D), a ligand for Plexin-B1, whereas expression of Plexin-B1 alone or coexpression of Rnd1 and a Rnd1 interaction-defective mutant of Plexin-B1 did not. The Sema4D-induced contraction in Plexin-B1/Rnd1-expressing COS-7 cells was suppressed by dominant negative RhoA, a Rho-associated kinase inhibitor, a dominant negative form of PDZ-RhoGEF, or deletion of the carboxyl-terminal PDZ-RhoGEF-binding region of Plexin-B1, indicating that the PDZ-RhoGEF/RhoA/Rho-associated kinase pathway is involved in this morphological effect. We also found that Rnd1 promoted the interaction between Plexin-B1 and PDZ-RhoGEF and thereby dramatically potentiated the Plexin-B1-mediated RhoA activation. We propose that Rnd1 plays an important role in the regulation of Plexin-B1 signaling, leading to Rho activation during axon guidance and cell migration.
Received for publication, March 25, 2003
* This work was supported in part by grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan and a grant from Takeda Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
These authors contributed equally to this work.
To whom correspondence should be addressed: Laboratory of Molecular
Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto
606-8502, Japan. Tel.: 81-75-753-4547; Fax: 81-75-753-7688; E-mail:
mnegishi{at}pharm.kyoto-u.ac.jp.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Tong, P. Chugha, P. K. Hota, R. S. Alviani, M. Li, W. Tempel, L. Shen, H.-W. Park, and M. Buck Binding of Rac1, Rnd1, and RhoD to a Novel Rho GTPase Interaction Motif Destabilizes Dimerization of the Plexin-B1 Effector Domain J. Biol. Chem., December 21, 2007; 282(51): 37215 - 37224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Basile, J. Gavard, and J. S. Gutkind Plexin-B1 Utilizes RhoA and Rho Kinase to Promote the Integrin-dependent Activation of Akt and ERK and Endothelial Cell Motility J. Biol. Chem., November 30, 2007; 282(48): 34888 - 34895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. G.-W. Wong, T. Nitkunan, I. Oinuma, C. Zhou, V. Blanc, R. S. D. Brown, S. R. J. Bott, J. Nariculam, G. Box, P. Munson, et al. Plexin-B1 mutations in prostate cancer PNAS, November 27, 2007; 104(48): 19040 - 19045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Ayoob, J. R. Terman, and A. L. Kolodkin Drosophila Plexin B is a Sema-2a receptor required for axon guidance Development, June 1, 2006; 133(11): 2125 - 2135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Oinuma, H. Katoh, and M. Negishi Semaphorin 4D/Plexin-B1-mediated R-Ras GAP activity inhibits cell migration by regulating {beta}1 integrin activity J. Cell Biol., May 22, 2006; 173(4): 601 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Togashi, E. F. Schmidt, and S. M. Strittmatter RanBPM contributes to Semaphorin3A signaling through plexin-A receptors. J. Neurosci., May 3, 2006; 26(18): 4961 - 4969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tanaka, H. Katoh, and M. Negishi Pragmin, a Novel Effector of Rnd2 GTPase, Stimulates RhoA Activity J. Biol. Chem., April 14, 2006; 281(15): 10355 - 10364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Barberis, A. Casazza, R. Sordella, S. Corso, S. Artigiani, J. Settleman, P. M. Comoglio, and L. Tamagnone p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling J. Cell Sci., October 15, 2005; 118(20): 4689 - 4700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Basile, T. Afkhami, and J. S. Gutkind Semaphorin 4D/Plexin-B1 Induces Endothelial Cell Migration through the Activation of PYK2, Src, and the Phosphatidylinositol 3-Kinase-Akt Pathway Mol. Cell. Biol., August 15, 2005; 25(16): 6889 - 6898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Harada, H. Katoh, and M. Negishi Direct Interaction of Rnd1 with FRS2{beta} Regulates Rnd1-induced Down-regulation of RhoA Activity and Is Involved in Fibroblast Growth Factor-induced Neurite Outgrowth in PC12 Cells J. Biol. Chem., May 6, 2005; 280(18): 18418 - 18424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-E. Govek, S. E. Newey, and L. Van Aelst The role of the Rho GTPases in neuronal development Genes & Dev., January 1, 2005; 19(1): 1 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Oinuma, H. Katoh, and M. Negishi Molecular Dissection of the Semaphorin 4D Receptor Plexin-B1-Stimulated R-Ras GTPase-Activating Protein Activity and Neurite Remodeling in Hippocampal Neurons J. Neurosci., December 15, 2004; 24(50): 11473 - 11480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Kreibich, S. H. Chalasani, and J. A. Raper The Neurotransmitter Glutamate Reduces Axonal Responsiveness to Multiple Repellents through the Activation of Metabotropic Glutamate Receptor 1 J. Neurosci., August 11, 2004; 24(32): 7085 - 7095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Oinuma, Y. Ishikawa, H. Katoh, and M. Negishi The Semaphorin 4D Receptor Plexin-B1 Is a GTPase Activating Protein for R-Ras Science, August 6, 2004; 305(5685): 862 - 865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Turner, S. Nicholls, and A. Hall The Activity of the Plexin-A1 Receptor Is Regulated by Rac J. Biol. Chem., August 6, 2004; 279(32): 33199 - 33205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Basile, A. Barac, T. Zhu, K.-L. Guan, and J. S. Gutkind Class IV Semaphorins Promote Angiogenesis by Stimulating Rho-Initiated Pathways through Plexin-B Cancer Res., August 1, 2004; 64(15): 5212 - 5224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li and K.-L. Guan The Down Syndrome Cell Adhesion Molecule (DSCAM) Interacts with and Activates Pak J. Biol. Chem., July 30, 2004; 279(31): 32824 - 32831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Swiercz, R. Kuner, and S. Offermanns Plexin-B1/RhoGEF-mediated RhoA activation involves the receptor tyrosine kinase ErbB-2 J. Cell Biol., June 21, 2004; 165(6): 869 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kakimoto, H. Katoh, and M. Negishi Identification of Splicing Variants of Rapostlin, a Novel Rnd2 Effector that Interacts with Neural Wiskott-Aldrich Syndrome Protein and Induces Neurite Branching J. Biol. Chem., April 2, 2004; 279(14): 14104 - 14110. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
