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J. Biol. Chem., Vol. 278, Issue 28, 25964-25969, July 11, 2003

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Interaction of Glycoprotein H of Human Herpesvirus 6 with the Cellular Receptor CD46^*

Fabio Santoro  , Heather L. Greenstone , Alessandra Insinga , M. Kathryn Liszewski ¶, John P. Atkinson ¶, Paolo Lusso  || and Edward A. Berger  **

From the Laboratory of Viral Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892, the Unit of Human Virology, Department of Biological and Technical Research, San Raffaele Scientific Institute, 20132 Milano, Italy, the ^¶Division of Rheumatology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110 and the ^||Department of Medical Sciences, University of Cagliari Medical School, 09100 Cagliari, Italy

Human herpesvirus 6 (HHV-6) employs the complement regulator CD46 (membrane cofactor protein) as a receptor for fusion and entry into target cells. Like other known herpesviruses, HHV-6 encodes multiple glycoproteins, several of which have been implicated in the entry process. In this report, we present evidence that glycoprotein H (gH) is the viral component responsible for binding to CD46. Antibodies to CD46 co-immunoprecipitated an 110-kDa protein band specifically associated with HHV-6-infected cells. This protein was identified as gH by selective depletion with an anti-gH monoclonal antibody, as well as by immunoblot analysis with a rabbit hyperimmune serum directed against a gH synthetic peptide. In reciprocal experiments, a monoclonal antibody against HHV-6 gH was found to co-immunoprecipitate CD46. Studies using monoclonal antibodies directed against specific CD46 domains, as well as engineered constructs lacking defined CD46 regions, demonstrated a close correspondence between the CD46 domains involved in the interaction with gH and those previously shown to be critical for HHV-6 fusion (i.e. short consensus repeats 2 and 3).

Received for publication, March 7, 2003 , and in revised form, April 29, 2003.

^* This work was supported in part by Grants 50C17 and 50D17 from the Third and Fourth AIDS Projects, Instituto Superiore di Sanit (National Institute of Health), Rome and by National Institutes of Health Grant R01 AI37618. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^** To whom correspondence should be addressed: Bldg. 4, Rm. 237, National Institutes of Health, Bethesda, MD 20892. Tel.: 301-402-2481; Fax: 301-480-1147; E-mail: edward_berger{at}nih.gov.

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