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J. Biol. Chem., Vol. 278, Issue 29, 26564-26571, July 18, 2003

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Presence of Multiple mRNA Cycling Sequence Element-binding Proteins in Crithidia fasciculata^*

Bidyottam Mittra, Krishna M. Sinha, Jane C. Hines and Dan S. Ray 

From the Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095

A consensus sequence present in the 5'- or 3'-untranslated regions of several Crithidia fasciculata messenger RNAs encoding proteins involved in DNA metabolism has been shown to be necessary for the periodic accumulation of these mRNAs during the cell cycle. A protein complex termed cycling sequence-binding protein (CSBP) has two subunits, CSBPA and CSBPB, and binds the consensus sequence with high specificity. The binding activity of CSBP was shown to vary during the cell cycle in parallel with the levels of putative target mRNAs. Although disruption of the CSBPA gene resulted in loss of both CSBPA and CSBPB, the putative target message levels still continued to vary during the cell cycle. The presence of an additional and distinct binding activity was revealed in these CSBPA null mutant cells. This activity, termed CSBP II, was also expressed in wild-type Crithidia cells. CSBP II has higher binding specificity for the cycling sequence element than the earlier described CSBP complex. Three polypeptides associated with purified CSBP II show specific binding to the cycling sequence. These proteins may represent a family of sequence-specific RNA-binding proteins involved in post-transcriptional regulation.

Received for publication, April 24, 2003

^* This work was supported by research Grant GM53254 from National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: 301A Paul D. Boyer Hall, 611 Charles Young Dr. E., UCLA, Los Angeles, CA 90095-1570. Tel.: 310-825-4178; Fax: 310-206-7286; E-mail: danray{at}ucla.edu.

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