HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 29, 26870-26878, July 18, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/29/26870    most recent M302405200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hendriks, E. F. Articles by Matthews, K. R. Search for Related Content PubMed PubMed Citation Articles by Hendriks, E. F. Articles by Matthews, K. R. Social Bookmarking                      What's this?

tbCPSF30 Depletion by RNA Interference Disrupts Polycistronic RNA Processing in Trypanosoma brucei^*

Edward F. Hendriks, Ammar Abdul-Razak and Keith R. Matthews 

From the School of Biological Sciences, Division of Biochemistry, 2.205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom

Gene expression in eukaryotes requires the post-transcriptional cleavage of mRNA precursors into mature mRNAs. In Trypanosoma brucei, mRNA processing is of particular importance, since most transcripts are derived from polycistronic transcription units. This organization dictates that regulated gene expression is promoter-independent and governed at the posttranscriptional level. We have identified tbCPSF30, a protein containing five CCCH zinc finger motifs, which is a homologue of the cleavage and polyadenylation specificity factor (CPSF) 30-kDa subunit, a component of the machinery required for 3'-end formation in yeast and mammals. Using gene silencing of tbCPSF30 by RNA interference, we demonstrate that this gene is essential in bloodstream and procyclic forms of T. brucei. Interestingly, tbCPSF30-specific RNA interference results in the accumulation of an aberrant tbCPSF30 mRNA species concomitant with depletion of tbCPSF30 protein. tbCPSF30 protein depletion is accompanied by the accumulation of unprocessed tubulin RNAs, implicating tbCPSF30 in polycistronic RNA processing. By genome data base mining, we also identify several other putative components of the T. brucei cleavage and polyadenylation machinery, indicating their conservation throughout eukaryotic evolution. This study is the first to identify and characterize a core component of the T. brucei CPSF and show its involvement in polycistronic RNA processing.

Received for publication, March 7, 2003 , and in revised form, April 15, 2003.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AY239358.

^* This work was funded by the Wellcome Trust. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

A Wellcome Trust University Fellow. To whom correspondence should be addressed. Tel.: 44-161-275-5083; Fax: 44-161-275-5082; E-mail: keith.matthews{at}man.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. R. Haanstra, M. Stewart, V.-D. Luu, A. van Tuijl, H. V. Westerhoff, C. Clayton, and B. M. Bakker Control and Regulation of Gene Expression: QUANTITATIVE ANALYSIS OF THE EXPRESSION OF PHOSPHOGLYCERATE KINASE IN BLOODSTREAM FORM TRYPANOSOMA BRUCEI J. Biol. Chem., February 1, 2008; 283(5): 2495 - 2507. [Abstract] [Full Text] [PDF]

				C. Hartmann, C. Benz, S. Brems, L. Ellis, V.-D. Luu, M. Stewart, I. D'Orso, C. Busold, K. Fellenberg, A. C. C. Frasch, et al. Small Trypanosome RNA-Binding Proteins TbUBP1 and TbUBP2 Influence Expression of F-Box Protein mRNAs in Bloodstream Trypanosomes Eukaryot. Cell, November 1, 2007; 6(11): 1964 - 1978. [Abstract] [Full Text] [PDF]

				C.-H. Li, H. Irmer, D. Gudjonsdottir-Planck, S. Freese, H. Salm, S. Haile, A. M. Estevez, and C. Clayton Roles of a Trypanosoma brucei 5'->3' exoribonuclease homolog in mRNA degradation RNA, December 1, 2006; 12(12): 2171 - 2186. [Abstract] [Full Text] [PDF]

				H. WEBB, R. BURNS, N. KIMBLIN, L. ELLIS, and M. CARRINGTON A novel strategy to identify the location of necessary and sufficient cis-acting regulatory mRNA elements in trypanosomes RNA, July 1, 2005; 11(7): 1108 - 1116. [Abstract] [Full Text] [PDF]

				H. Webb, R. Burns, L. Ellis, N. Kimblin, and M. Carrington Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor Nucleic Acids Res., March 8, 2005; 33(5): 1503 - 1512. [Abstract] [Full Text] [PDF]

				G. M. Zeiner, R. A. Hitchcock, N. R. Sturm, and D. A. Campbell 3'-End Polishing of the Kinetoplastid Spliced Leader RNA Is Performed by SNIP, a 3'->5' Exonuclease with a Motley Assortment of Small RNA Substrates Mol. Cell. Biol., December 1, 2004; 24(23): 10390 - 10396. [Abstract] [Full Text] [PDF]

				P. Ganot, T. Kallesoe, R. Reinhardt, D. Chourrout, and E. M. Thompson Spliced-Leader RNA trans Splicing in a Chordate, Oikopleura dioica, with a Compact Genome Mol. Cell. Biol., September 1, 2004; 24(17): 7795 - 7805. [Abstract] [Full Text] [PDF]