JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M209713200 on November 4, 2002

J. Biol. Chem., Vol. 278, Issue 3, 1713-1720, January 17, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/3/1713    most recent
M209713200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Newport, G.
Right arrow Articles by Agabian, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Newport, G.
Right arrow Articles by Agabian, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Inactivation of Kex2p Diminishes the Virulence of Candida albicans*

George NewportDagger §, Alan KuoDagger §, Amy Flattery||, Charles Gill||, Julie J. BlakeDagger **, Myra B. Kurtz||, George K. Abruzzo||, and Nina AgabianDagger Dagger Dagger

From the Dagger  Department of Stomatology, University of California at San Francisco, California 94143-0422 and || Merck Research Laboratories, Rahway, New Jersey 07065-0900

Deletion of the kexin gene (KEX2) in Candida albicans has a pleiotropic effect on phenotype and virulence due partly to a defect in the expression of two major virulence factors: the secretion of active aspartyl proteinases and the formation of hyphae. kex2/kex2 mutants are highly attenuated in a mouse systemic infection model and persist within cultured macrophages for at least 24 h without causing damage. Pathology is modest, with little disruption of kidney matrix. The infecting mutant cells are largely confined to glomeruli, and are aberrant in morphology. The complex phenotype of the deletion mutants reflects a role for kexin in a wide range of cellular processes. Taking advantage of the specificity of Kex2p cleavage, an algorithm we developed to scan the 9168 open reading frames in Assembly 6 of the C. albicans genome identified 147 potential substrates of Kex2p. These include all previously identified substrates, including eight secreted aspartyl proteinases, the exoglucanase Xog1p, the immunodominant antigen Mp65, and the adhesin Hwp1p. Other putative Kex2p substrates identified include several adhesins, cell wall proteins, and hydrolases previously not implicated in pathogenesis. Kexins also process fungal mating pheromones; a modification of the algorithm identified a putative mating pheromone with structural similarities to Saccharomyces cerevisiae alpha -factor.

* This study was supported by National Institutes of Health Grants A133317, RO1DE12940, P01 DE07946-15S2, and PO1 DE07946.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

Supported by University of California University-wide AIDS Research Program Grant F97-SF-048.

** Present address: Incyte Genomics, Palo Alto, CA 94304.

Dagger Dagger To whom correspondence should be addressed: Dept. of Stomatology, University of California, 521 Parnassus Avenue, Box 0422, San Francisco, CA 94243-0422. Tel.: 415-476-6845; Fax: 415-476-0664; Email: agabian@itsa.ucsf.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
E. Hiller, S. Heine, H. Brunner, and S. Rupp
Candida albicans Sun41p, a Putative Glycosidase, Is Involved in Morphogenesis, Cell Wall Biogenesis, and Biofilm Formation
Eukaryot. Cell, November 1, 2007; 6(11): 2056 - 2065.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Albrecht, A. Felk, I. Pichova, J. R. Naglik, M. Schaller, P. de Groot, D. MacCallum, F. C. Odds, W. Schafer, F. Klis, et al.
Glycosylphosphatidylinositol-anchored Proteases of Candida albicans Target Proteins Necessary for Both Cellular Processes and Host-Pathogen Interactions
J. Biol. Chem., January 13, 2006; 281(2): 688 - 694.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
B. L. Granger, M. L. Flenniken, D. A. Davis, A. P. Mitchell, and J. E. Cutler
Yeast wall protein 1 of Candida albicans
Microbiology, May 1, 2005; 151(5): 1631 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Brand, D. M. MacCallum, A. J. P. Brown, N. A. R. Gow, and F. C. Odds
Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus
Eukaryot. Cell, August 1, 2004; 3(4): 900 - 909.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
O. Mizutani, A. Nojima, M. Yamamoto, K. Furukawa, T. Fujioka, Y. Yamagata, K. Abe, and T. Nakajima
Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae
Eukaryot. Cell, August 1, 2004; 3(4): 1036 - 1048.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
H. E. Ambrose, S. P. Keely, E. M. Aliouat, E. Dei-Cas, A. E. Wakefield, R. F. Miller, and J. R. Stringer
Expression and complexity of the PRT1 multigene family of Pneumocystis carinii
Microbiology, February 1, 2004; 150(2): 293 - 300.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. L. Panwar, M. Legrand, D. Dignard, M. Whiteway, and Paul. T. Magee
MF{alpha}1, the Gene Encoding the {alpha} Mating Pheromone of Candida albicans
Eukaryot. Cell, December 1, 2003; 2(6): 1350 - 1360.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. J. Bennett, M. A. Uhl, M. G. Miller, and A. D. Johnson
Identification and Characterization of a Candida albicans Mating Pheromone
Mol. Cell. Biol., November 15, 2003; 23(22): 8189 - 8201.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. J. Warenda, S. Kauffman, T. P. Sherrill, J. M. Becker, and J. B. Konopka
Candida albicans Septin Mutants Are Defective for Invasive Growth and Virulence
Infect. Immun., July 1, 2003; 71(7): 4045 - 4051.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.