HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 30, 27372-27381, July 25, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/30/27372    most recent M301110200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dziak, R. Articles by Yankulov, K. Search for Related Content PubMed PubMed Citation Articles by Dziak, R. Articles by Yankulov, K. Social Bookmarking                      What's this?

Evidence for a Role of MCM (Mini-chromosome Maintenance)5 in Transcriptional Repression of Sub-telomeric and Ty-proximal Genes in Saccharomyces cerevisiae^*

Renata Dziak  , David Leishman  , Maja Radovic , Bik K. Tye ¶ and Krassimir Yankulov  ||

From the Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada and the ^¶Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14583

The MCM (mini-chromosome maintenance) genes have a well established role in the initiation of DNA replication and in the elongation of replication forks in Saccharomyces cerevisiae. In this study we demonstrate elevated expression of sub-telomeric and Ty retrotransposon-proximal genes in two mcm5 strains. This pattern of up-regulated genes resembles the genome-wide association of MCM proteins to chromatin that was reported earlier. We link the altered gene expression in mcm5 strains to a reversal of telomere position effect (TPE) and to remodeling of sub-telomeric and Ty chromatin. We also show a suppression of the Ts phenotype of a mcm5 strain by the high copy expression of the TRA1 component of the chromatin-remodeling SAGA/ADA (SPT-ADA-GCN5 acetylase/ADAptor). We propose that MCM proteins mediate the establishment of silent chromatin domains around telomeres and Ty retrotransposons.

Received for publication, January 31, 2003 , and in revised form, April 29, 2003.

^* This study was supported by Grant MOP-36371 from the Canadian Institutes of Health Research (to K. Y.) and by the National Sciences and Engineering Research Council of Canada (to K. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

^|| To whom correspondence should be addressed. Tel.: 619-824-4120 (ext. 56466); Fax: 519-837-2075; E-mail: yankulov{at}uoguelph.ca.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. Liachko and B. K. Tye Mcm10 Mediates the Interaction Between DNA Replication and Silencing Machineries Genetics, February 1, 2009; 181(2): 379 - 391. [Abstract] [Full Text] [PDF]

				M. A. Rehman, D. Wang, G. Fourel, E. Gilson, and K. Yankulov Subtelomeric ACS-containing Proto-silencers Act as Antisilencers in Replication Factors Mutants in Saccharomyces cerevisiae Mol. Biol. Cell, January 1, 2009; 20(2): 631 - 641. [Abstract] [Full Text] [PDF]

				T. van Welsem, F. Frederiks, K. F. Verzijlbergen, A. W. Faber, Z. W. Nelson, D. A. Egan, D. E. Gottschling, and F. van Leeuwen Synthetic Lethal Screens Identify Gene Silencing Processes in Yeast and Implicate the Acetylated Amino Terminus of Sir3 in Recognition of the Nucleosome Core Mol. Cell. Biol., June 1, 2008; 28(11): 3861 - 3872. [Abstract] [Full Text] [PDF]

				X. Q. Ge, D. A. Jackson, and J. J. Blow Dormant origins licensed by excess Mcm2 7 are required for human cells to survive replicative stress Genes & Dev., December 15, 2007; 21(24): 3331 - 3341. [Abstract] [Full Text] [PDF]

				M. A. Rehman, G. Fourel, A. Mathews, D. Ramdin, M. Espinosa, E. Gilson, and K. Yankulov Differential Requirement of DNA Replication Factors for Subtelomeric ARS Consensus Sequence Protosilencers in Saccharomyces cerevisiae Genetics, December 1, 2006; 174(4): 1801 - 1810. [Abstract] [Full Text] [PDF]

				E. B. Gomez, J. M. Espinosa, and S. L. Forsburg Schizosaccharomyces pombe mst2+ Encodes a MYST Family Histone Acetyltransferase That Negatively Regulates Telomere Silencing Mol. Cell. Biol., October 15, 2005; 25(20): 8887 - 8903. [Abstract] [Full Text] [PDF]

				I. Liachko and B. K. Tye Mcm10 Is Required for the Maintenance of Transcriptional Silencing in Saccharomyces cerevisiae Genetics, October 1, 2005; 171(2): 503 - 515. [Abstract] [Full Text] [PDF]

				S. Addya, M. A. Keller, K. Delgrosso, C. M. Ponte, R. Vadigepalli, G. E. Gonye, and S. Surrey Erythroid-induced commitment of K562 cells results in clusters of differentially expressed genes enriched for specific transcription regulatory elements Physiol Genomics, September 16, 2004; 19(1): 117 - 130. [Abstract] [Full Text] [PDF]

				Y. Kinoshita and E. M. Johnson Site-specific Loading of an MCM Protein Complex in a DNA Replication Initiation Zone Upstream of the c-MYC Gene in the HeLa Cell Cycle J. Biol. Chem., August 20, 2004; 279(34): 35879 - 35889. [Abstract] [Full Text] [PDF]

				S. L. Forsburg Eukaryotic MCM Proteins: Beyond Replication Initiation Microbiol. Mol. Biol. Rev., March 1, 2004; 68(1): 109 - 131. [Abstract] [Full Text] [PDF]