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J. Biol. Chem., Vol. 278, Issue 30, 27766-27771, July 25, 2003
Lysophosphatidylcholine Acts as an Anti-hemostatic Molecule in the Saliva of the Blood-sucking Bug Rhodnius prolixus*![]() From the Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, P.O. Box 68041, Cidade Universitária, Ilha do Fundão, Avenida Bauhínia 400, Rio de Janeiro, CEP-21941-590, Rio de Janeiro, Brazil
Blood-sucking arthropods possess a variety of anti-hemostatic factors in
their salivary glands to maintain blood fluidity during feeding. In this work
we demonstrate the anti-hemostatic properties of lysophosphatidylcholine
(lysoPC) isolated from the salivary glands of Rhodnius prolixus.
First, we examined salivary glands of fourth and fifth instar nymphs for their
phospholipid composition. The lumen displayed an accumulation of its
phospholipid content, mainly phosphatidylcholine and lysoPC, with a 6-fold
increase for the latter. To determine the presence of phospholipids in the
saliva, fourth instar nymphs were fed with a32P-enriched blood
meal. After 28 days their saliva was collected and subjected to lipid
extraction, thin-layer chromatography, and autoradiography. The results showed
the presence in the saliva of the same phospholipids present in the lumen. We
then examined possible biological roles of these phospholipids when compared
with other known effects of lysoPC. The luminal lipid extract and purified
lysoPC from the lumen and saliva were tested for inhibition of washed rabbit
platelets' aggregation induced by
Received for publication, December 6, 2002 , and in revised form, May 9, 2003. * This work was supported by grants from the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-PADCT, Universal), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo à Pesquisa Carlos Chagas Filho do Estado do Rio de Janeiro (FAPERJ) and by Grant F/2887-1 from the International Foundation for Science, Stockholm, Sweden (to M. A. C. S.-N.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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