HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 30, 28237-28245, July 25, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/30/28237    most recent M302651200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stelzl, U. Articles by Patel, D. J. Search for Related Content PubMed PubMed Citation Articles by Stelzl, U. Articles by Patel, D. J. Social Bookmarking                      What's this?

RNA-structural Mimicry in Escherichia coli Ribosomal Protein L4-dependent Regulation of the S10 Operon^*

Ulrich Stelzl   ¶, Janice M. Zengel ||, Marina Tovbina , Marquis Walker ||, Knud H. Nierhaus , Lasse Lindahl || and Dinshaw J. Patel 

From the Memorial Sloan Kettering Cancer Center, Cellular Biochemistry and Biophysics Program, New York, New York 10021, the Max-Planck-Institut für Molekulare Genetik, AG Ribosomen, Ihnestrasse 73, D-14195 Berlin, Germany, and the ^||Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, Maryland 21250

Ribosomal protein L4 regulates the 11-gene S10 operon in Escherichia coli by acting, in concert with transcription factor NusA, to cause premature transcription termination at a Rho-independent termination site in the leader sequence. This process presumably involves L4 interaction with the leader mRNA. Here, we report direct, specific, and independent binding of ribosomal protein L4 to the S10 mRNA leader in vitro. Most of the binding energy is contributed by a small hairpin structure within the leader region, but a 64-nucleotide sequence is required for the bona fide interaction. Binding to the S10 leader mRNA is competed by the 23 S rRNA L4 binding site. Although the secondary structures of the mRNA and rRNA binding sites appear different, phosphorothioate footprinting of the L4-RNA complexes reveals close structural similarity in three dimensions. Mutational analysis of the mRNA binding site is compatible with the structural model. In vitro binding of L4 induces structural changes of the S10 leader RNA, providing a first clue for how protein L4 may provoke transcription termination.

Received for publication, March 14, 2003 , and in revised form, April 28, 2003.

^* This work was supported by the National Institutes of Health Grant CA46778 (to D. J. P.) and NIGMS, National Institutes of General Medical Science Grant 54876 (to J. M. Z. and L. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Present address: Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Straße 10, D-13092 Berlin-Buch, Germany. Tel.: 49-30-9406-2354; Fax: 49-30-9406-2552; E-mail: u.stelzl{at}mdc-berlin.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Meskauskas and J. D. Dinman Ribosomal protein L3 functions as a 'rocker switch' to aid in coordinating of large subunit-associated functions in eukaryotes and Archaea Nucleic Acids Res., November 1, 2008; 36(19): 6175 - 6186. [Abstract] [Full Text] [PDF]

				L. V. Aseev, A. A. Levandovskaya, L. S. Tchufistova, N. V. Scaptsova, and I. V. Boni A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo RNA, September 1, 2008; 14(9): 1882 - 1894. [Abstract] [Full Text] [PDF]

				N. Choonee, S. Even, L. Zig, and H. Putzer Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism Nucleic Acids Res., March 12, 2007; 35(5): 1578 - 1588. [Abstract] [Full Text] [PDF]

				M. Wels, C. Francke, R. Kerkhoven, M. Kleerebezem, and R. J. Siezen Predicting cis-acting elements of Lactobacillus plantarum by comparative genomics with different taxonomic subgroups Nucleic Acids Res., April 13, 2006; 34(7): 1947 - 1958. [Abstract] [Full Text] [PDF]

				T. D. Allen, T. Watkins, L. Lindahl, and J. M. Zengel Regulation of Ribosomal Protein Synthesis in Vibrio cholerae J. Bacteriol., September 1, 2004; 186(17): 5933 - 5937. [Abstract] [Full Text] [PDF]