HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 31, 29145-29152, August 1, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/31/29145    most recent M303746200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Naylor, M. J. Articles by Ormandy, C. J. Search for Related Content PubMed PubMed Citation Articles by Naylor, M. J. Articles by Ormandy, C. J. Social Bookmarking                      What's this?

The Neuropeptide Galanin Augments Lobuloalveolar Development^*

Matthew J. Naylor  , Erika Ginsburg ¶, Tiina P. Iismaa ||, Barbara K. Vonderhaar ¶, David Wynick ** and Christopher J. Ormandy  

From the Development Group, Cancer Research Program and ^||Neurobiology Program, Garvan Institute of Medical Research, St Vincent's Hospital, Sydney, New South Wales 2010, Australia, ^¶Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, and ^**University Research Centre Neuroendocrinology, Bristol University, Marlborough Street, Bristol BS2 8HW, United Kingdom

Mammary lobuloalveolar development during pregnancy is controlled by ovarian sex steroids and pituitary prolactin release. In organ culture these hormones are incapable of reproducing the density and size of lobuloalveoli seen in mice, suggesting the existence of other undiscovered factors. We showed previously that galanin knockout mice fail to lactate sufficiently for pup survival following their first pregnancy. Here we demonstrate that prolactin treatment of galanin knockout mice allows pup survival but does not completely rescue lobuloalveolar development or reduced milk protein expression. When galanin was used in combination with prolactin in mammary organ culture, larger and more numerous lobules were produced than with prolactin alone. Galanin alone produced sustained activation of STAT5a and the induction of milk protein expression but did not induce lobulogenesis. Examination of the transcriptional interaction between galanin and prolactin using oligonucleotide microarrays demonstrated synergistic and antagonistic modes of interaction between these hormones. These data establish a new role for galanin as a hormone augmenting mammary development during pregnancy in concert with prolactin.

Received for publication, April 10, 2003 , and in revised form, May 15, 2003.

^* This work was supported in part by Congressionally Directed Medical Research Program Grant DAMD 17-99-1-9185 (to C. J. O.) and by grants from the Cancer Council of New South Wales and the Australian National Health and Medical Research Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a University of New South Wales, Faculty of Medicine, Dean's Research Scholarship.

To whom correspondence should be addressed. Tel.: 612-9295-8329; Fax: 612-9295-8321; E-mail: c.ormandy{at}garvan.org.au.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. J. Naylor, N. Li, J. Cheung, E. T. Lowe, E. Lambert, R. Marlow, P. Wang, F. Schatzmann, T. Wintermantel, G. Schuetz, et al. Ablation of {beta}1 integrin in mammary epithelium reveals a key role for integrin in glandular morphogenesis and differentiation J. Cell Biol., November 21, 2005; 171(4): 717 - 728. [Abstract] [Full Text] [PDF]

				M. J. Naylor, S. R. Oakes, M. Gardiner-Garden, J. Harris, K. Blazek, T. W. C. Ho, F. C. Li, D. Wynick, A. M. Walker, and C. J. Ormandy Transcriptional Changes Underlying the Secretory Activation Phase of Mammary Gland Development Mol. Endocrinol., July 1, 2005; 19(7): 1868 - 1883. [Abstract] [Full Text] [PDF]

				Genetically Modified Animals in Endocrinology Endocr. Rev., August 1, 2004; 25(4): 673 - 677. [Full Text] [PDF]