HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 32, 29667-29675, August 8, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/32/29667    most recent M301292200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cao, C. Articles by Kufe, D. Search for Related Content PubMed PubMed Citation Articles by Cao, C. Articles by Kufe, D. Social Bookmarking                      What's this?

Catalase Activity Is Regulated by c-Abl and Arg in the Oxidative Stress Response^*

Cheng Cao  , Yumei Leng  and Donald Kufe  ¶

From the Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115 and the Beijing Institute of Biotechnology, Beijing 100850, People's Republic of China

The Abl family of mammalian non-receptor tyrosine kinases includes c-Abl and Arg. Recent studies have demonstrated that c-Abl and Arg are activated in the response of cells to oxidative stress. This work demonstrates that catalase, a major effector of the cellular defense against H₂O₂, interacts with c-Abl and Arg. The results show that H₂O₂ induced binding of c-Abl and Arg to catalase. The SH3 domains of c-Abl and Arg bound directly to catalase at a P²⁹³FNP site. c-Abl and Arg phosphorylated catalase at Tyr²³¹ and Tyr³⁸⁶ in vitro and in the response of cells to H₂O₂. The functional significance of the interaction is supported by the demonstration that cells deficient in both c-Abl and Arg exhibit substantial increases in H₂O₂ levels. In addition, c-abl^–/– arg^–/– cells exhibited a marked increase in H₂O₂-induced apoptosis compared with that found in the absence of either kinase. These findings indicate that c-Abl and Arg regulate catalase and that this signaling pathway is of importance to apoptosis in the oxidative stress response.

Received for publication, February 5, 2003 , and in revised form, May 29, 2003.

^* This work was supported by NCI Grants CA42802 and CA49639 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dana-Farber Cancer Inst., Harvard Medical School, Boston, MA 02115. Tel.: 617-632-3141; Fax: 617-632-2934; E-mail: donald_kufe{at}dfci.harvard.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Z. Imam, F. E. Indig, W.-H. Cheng, S. P. Saxena, T. Stevnsner, D. Kufe, and V. A. Bohr Cockayne syndrome protein B interacts with and is phosphorylated by c-Abl tyrosine kinase Nucleic Acids Res., August 1, 2007; 35(15): 4941 - 4951. [Abstract] [Full Text] [PDF]

				S. G. Menon, E. H. Sarsour, A. L. Kalen, S. Venkataraman, M. J. Hitchler, F. E. Domann, L. W. Oberley, and P. C. Goswami Superoxide Signaling Mediates N-acetyl-L-cysteine-Induced G1 Arrest: Regulatory Role of Cyclin D1 and Manganese Superoxide Dismutase Cancer Res., July 1, 2007; 67(13): 6392 - 6399. [Abstract] [Full Text] [PDF]

				Z.-L. Xiao, J. Amaral, P. Biancani, and J. Behar Impaired cytoprotective function of muscle in human gallbladders with cholesterol stones Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G525 - G532. [Abstract] [Full Text] [PDF]

				J. He, Y. Tohyama, K.-i. Yamamoto, M. Kobayashi, Y. Shi, T. Takano, C. Noda, K. Tohyama, and H. Yamamura Lysosome is a primary organelle in B cell receptor-mediated apoptosis: an indispensable role of Syk in lysosomal function Genes Cells, January 1, 2005; 10(1): 23 - 35. [Abstract] [Full Text] [PDF]

				Y. Fukamatsu, N. Yabe, and K. Hasunuma Arabidopsis NDK1 is a Component of ROS Signaling by Interacting with Three Catalases Plant Cell Physiol., October 15, 2003; 44(10): 982 - 989. [Abstract] [Full Text] [PDF]

				C. Cao, Y. Leng, W. Huang, X. Liu, and D. Kufe Glutathione Peroxidase 1 Is Regulated by the c-Abl and Arg Tyrosine Kinases J. Biol. Chem., October 10, 2003; 278(41): 39609 - 39614. [Abstract] [Full Text] [PDF]