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J. Biol. Chem., Vol. 278, Issue 32, 30193-30198, August 8, 2003

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Novel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia coli^*

Pedro Echave , Jordi Tamarit, Elisa Cabiscol and Joaquim Ros 

From the Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, 25198 Lleida, Spain

Alcohol dehydrogenase E (AdhE) is an Fe-enzyme that, under anaerobic conditions, is involved in dissimilation of glucose. The enzyme is also present under aerobic conditions, its amount is about one-third and its activity is only one-tenth of the values observed under anaerobic conditions. Nevertheless, its function in the presence of oxygen remained ignored. The data presented in this paper led us to propose that the enzyme has a protective role against oxidative stress. Our results indicated that cells deleted in adhE gene could not grow aerobically in minimal media, were extremely sensitive to oxidative stress and showed division defects. In addition, compared with wild type, mutant cells displayed increased levels of internal peroxides (even higher than those found in a katG strain) and increased protein carbonyl content. This pleiotropic phenotype disappeared when the adhE gene was reintroduced into the defective strain. The purified enzyme was highly reactive with hydrogen peroxide (with a K_i of 5 µM), causing inactivation due to a metal-catalyzed oxidation reaction. It is possible to prevent this reactivity to hydrogen peroxide by zinc, which can replace the iron atom at the catalytic site of AdhE. This can also be achieved by addition of ZnSO₄ to cell cultures. In such conditions, addition of hydrogen peroxide resulted in reduced cell viability compared with that obtained without the Zn treatment. We therefore propose that AdhE acts as a H₂O₂ scavenger in Escherichia coli cells grown under aerobic conditions.

Received for publication, April 25, 2003 , and in revised form, May 30, 2003.

^* This work was funded by Grants BMC2001-0874 from Ministerio de Ciencia y Tecnología (Spain) and 2000 SGR 0042 from Generalitat de Catalunya (Spain). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

A recipient of a Ph.D. scholarship from the Ministerio de Educación y Cultura (Spain).

To whom correspondence should be addressed: Dept. Ciències Mèdiques Bàsiques, Facultat de Medicina, Av. Rovira Roure 44, 25198 Lleida, Spain. Tel.: 34-973-702-275; Fax: 34-973-702-426; E-mail: joaquim.ros{at}cmb.udl.es.

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