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J. Biol. Chem., Vol. 278, Issue 32, 30382-30393, August 8, 2003

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Reverse Reaction of Lysophosphatidylinositol Acyltransferase

FUNCTIONAL RECONSTITUTION OF COENZYME A-DEPENDENT TRANSACYLATION SYSTEM^*

Atsushi Yamashita , Masanobu Watanabe, Kazuaki Sato, Tomoyuki Miyashita, Tomonari Nagatsuka, Hironori Kondo, Norikazu Kawagishi, Hiroki Nakanishi, Ryo Kamata, Takayuki Sugiura and Keizo Waku

From the Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-0195, Japan

CoA-dependent transacylation activity in microsomes catalyzes the transfer of fatty acid between phospholipids and lysophospholipids in the presence of CoA without the generation of free fatty acid. We examined the mechanism of the transacylation system using partially purified acyl-CoA:lysophosphatidylinositol (LPI) acyltransferase (LPIAT) from rat liver microsomes to test our hypothesis that both the reverse and forward reactions of acyl-CoA:lysophospholipid acyltransferases are involved in the CoA-dependent transacylation process. The purified LPIAT fraction exhibited ATP-independent acyl-CoA synthetic activity and CoA-dependent LPI generation from PI, suggesting that LPIAT could operate in reverse to form acyl-CoA and LPI. CoA-dependent acylation of LPI by the purified LPIAT fraction required PI as the acyl donor. In addition, the combination of purified LPIAT and recombinant lysophosphatidic acid acyltransferase could reconstitute CoA-dependent transacylation between PI and phosphatidic acid. These results suggest that the CoA-dependent transacylation system consists of the following: 1) acyl-CoA synthesis from phospholipid through the reverse action of acyl-CoA:lysophospholipid acyltransferases; and 2) transfer of fatty acyl moiety from the newly formed acyl-CoA to lysophospholipid through the forward action of acyl-CoA:lysophospholipid acyltransferases.

Received for publication, April 2, 2003 , and in revised form, May 12, 2003.

^* This work was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Sciences, and Technology of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 81-426-85-3747; Fax: 81-426-85-1345; E-mail: ayamashi{at}pharm.teikyo-u.ac.jp.

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