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J. Biol. Chem., Vol. 278, Issue 35, 32905-32913, August 29, 2003

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Regulation of the NEDD8 Conjugation System by a Splicing Variant, NUB1L^*

Tomoaki Tanaka, Hidenori Kawashima, Edward T. H. Yeh and Tetsu Kamitani 

From the Department of Cardiology, The University of Texas M. D. Anderson Cancer Center and Institute of Molecular Medicine, The University of Texas-Houston Health Science Center, Houston, Texas 77030

NEDD8 is a ubiquitin-like protein that controls vital biological events through its conjugation to target proteins. We previously identified a negative regulator of the NEDD8 conjugation system, NUB1, which works by recruiting NEDD8 and its conjugates to the proteasome for degradation. Recently, we found its splicing variant, NUB1L. It possesses an insertion of 14 amino acids that codes for a UBA domain. Structural study revealed that NUB1 has a NEDD8-binding site at the C terminus, whereas NUB1L has an additional site at the newly generated UBA domain. Interestingly, the sequence A(X₄)L(X₁₀)L(X₃)L was conserved in these NEDD8-binding sites among human and other mammals. Mutational studies revealed that at least three Leu residues in the conserved sequence are required for binding with NEDD8. Functional study suggested that the NEDD8-binding ability at the C terminus of NUB1 and NUB1L is mainly involved in the down-regulation of NEDD8, but the NEDD8-binding ability at the UBA2 domain of NUB1L is minimally or not involved at all. The NEDD8-binding ability at the UBA2 domain might be required for an unknown function of NUB1L.

Received for publication, November 26, 2002 , and in revised form, June 19, 2003.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AF534114, AF459743, and AY129295.

^* This work was supported by National Institutes of Health Grant R01 DK56298 (to T. K.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Cardiology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 449, Houston, TX 77030. Tel.: 713-500-2348; Fax: 713-500-2325; E-mail: tkamitani{at}mdanderson.org.

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