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J. Biol. Chem., Vol. 278, Issue 35, 33239-33247, August 29, 2003

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A Novel Membrane-associated Glycovariant of BEHAB/Brevican Is Up-regulated during Rat Brain Development and in a Rat Model of Invasive Glioma^*

Mariano S. Viapiano, Russell T. Matthews  and Susan Hockfield

From the Department of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06510

BEHAB (brain-enriched hyaluronan-binding protein)/brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult rat brain. BEHAB/brevican expression is up-regulated coincident with glial cell proliferation and/or motility, including during early central nervous system development and in invasive glioma. An understanding of the molecular interactions that mediate BEHAB/brevican function is still in its infancy because of the existence of several BEHAB/brevican isoforms, each of which may mediate different functions. Here, we describe a novel BEHAB/brevican isoform, B/b₁₃₀, and demonstrate that it is neither the glycosylphosphatidylinositol-linked splice variant of BEHAB/brevican nor a cleavage product of the full-length protein (B/b₁₅₀). B/b₁₃₀ is an underglycosylated isoform of BEHAB/brevican, lacking glycosaminoglycan chains as well as most of the sugars that invest B/b₁₅₀. B/b₁₃₀ localizes exclusively to the particulate fraction of rat brain and associates with the cell membrane by a previously undescribed calcium-independent mechanism. In addition, B/b₁₃₀ is the major isoform of BEHAB/brevican that is up-regulated in a rat model of invasive glioma and may therefore contribute to the invasive ability of glioma cells. Further understanding of BEHAB/brevican isoforms will advance our knowledge of the function of this ECM component and may help identify new potential therapeutic targets for primary brain tumors.

Received for publication, April 3, 2003 , and in revised form, June 4, 2003.

^* This work was supported by National Institutes of Health Grant R01NS35228. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Neurobiology, Yale University School of Medicine, 333 Cedar St., SHM C405, New Haven, CT 06510. Tel.: 203-785-5941; Fax: 203-785-5263; E-mail: russell.matthews{at}yale.edu.

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				B. Hu, L. L. Kong, R. T. Matthews, and M. S. Viapiano The Proteoglycan Brevican Binds to Fibronectin after Proteolytic Cleavage and Promotes Glioma Cell Motility J. Biol. Chem., September 5, 2008; 283(36): 24848 - 24859. [Abstract] [Full Text] [PDF]

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