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J. Biol. Chem., Vol. 278, Issue 35, 33562-33570, August 29, 2003

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Assembly of Archaeal Cell Division Protein FtsZ and a GTPase-inactive Mutant into Double-stranded Filaments^*

María A. Oliva  , Sonia Huecas , Juan M. Palacios , Jaime Martín-Benito ¶, José M. Valpuesta ¶ and José M. Andreu  ||

From the Centro de Investigaciones Biológicas and the ^¶Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, E28006 Madrid, Spain

We have studied the assembly and GTPase of purified FtsZ from the hyperthermophilic archaeon Methanococcus jannaschii, a structural homolog of eukaryotic tubulin, employing wild-type FtsZ, FtsZ-His₆ (histidine-tagged FtsZ), and the new mutants FtsZ-W319Y and FtsZ-W319Y-His₆, with light scattering, nucleotide analyses, electron microscopy, and image processing methods. This has revealed novel properties of FtsZ. The GTPase of archaeal FtsZ polymers is suppressed in Na⁺-containing buffer, generating stabilized structures that require GDP addition for disassembly. FtsZ assembly is polymorphic. Archaeal FtsZ(wt) assembles into associated and isolated filaments made of two parallel protofilaments with a 43 Å longitudinal spacing between monomers, and this structure is also observed in bacterial FtsZ from Escherichia coli. The His₆ extension facilitates the artificial formation of helical tubes and sheets. FtsZ-W319Y-His₆ is an inactivated GTPase whose assembly remains regulated by GTP and Mg²⁺. It forms two-dimensional crystals made of symmetrical pairs of tubulin-like protofilaments, which associate in an antiparallel array (similarly to the known Ca²⁺-induced sheets of FtsZ-His₆). In contrast to the lateral interactions of microtubule protofilaments, we propose that the primary assembly product of FtsZ is the double-stranded filament, one or several of which might form the dynamic Z ring during prokaryotic cell division.

Received for publication, April 11, 2003 , and in revised form, June 4, 2003.

^* This work was supported by grants from MCyT BIO-1999-0859-c03-02 and BIO2002-03665, CAM 07B/0026/2002 (to J. M. A.), MCyT BMC2001-0950 (to J. M. V.), Programa de Grupos Estratégicos de la Comunidad de Madrid, and Red Temática de Investigación Cooperativa FIS C03/14 (CIB). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a predoctoral fellowship from FPI.

^|| To whom correspondence should be addressed: CIB, CSIC, Ramiro de Maeztu 9, 28020 Madrid, Spain. Fax: 34-91536042; E-mail: j.m.andreu{at}cib.csic.es.

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