HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 36, 33878-33886, September 5, 2003

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 278/36/33878    most recent M303749200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pédron, T. Articles by Sansonetti, P. J. Search for Related Content PubMed PubMed Citation Articles by Pédron, T. Articles by Sansonetti, P. J. Social Bookmarking                      What's this?

The Invasive Phenotype of Shigella flexneri Directs a Distinct Gene Expression Pattern in the Human Intestinal Epithelial Cell Line Caco-2^*,

Thierry Pédron , Christelle Thibault  and Philippe J. Sansonetti  ¶

From the Unité de Pathogénie Microbienne Moleculaire et Unité INSERM 389, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15 and the Institut de Génétique et de Biologie Moléculaire et Cellulaire,1 Rue Laurent Fries, 67404 ILLKIRCH Cedex, Strasbourg, France

Invasion of the human colonic epithelium by Shigella flexneri causes inflammation that disrupts the intestinal barrier. Invaded intestinal epithelial cells are the major source of mediators recruiting the inflammatory infiltrate. To better characterize the global response of intestinal epithelial cells to Shigella invasion, Caco-2 cells were infected by an invasive isolate of S. flexneri 5a, and their transcriptome was analyzed by Affymetrix (Santa Clara, CA) microarrays (12,000 genes) and compared with these elicited by a non-invasive Shigella mutant and tumor necrosis factor (TNF)-. The invasive and non-invasive strains enhanced transcription of a common pattern of 240 genes, among which genes encoding isoforms of cytochrome P-450 were induced. These genes were not induced by TNF-. Conversely, both the invasive strain and TNF- induced a common set of 18 genes, mainly encoding proinflammatory molecules. They also induced specific sets of genes. The transcriptome induced by the invasive strain was characterized by the induction of early genes (i.e. expressed within the first 45 min of invasion) and late genes (i.e. after 60 min of invasion) whose pattern was strongly biased toward stimulation of granulopoiesis, chemoattraction, activation, and adherence of polymorphonuclear leukocytes. When compared with a non-invasive Shigella and TNF-, invasive Shigella induced a narrow transcriptome that seems to program infected epithelial cells to recruit a mucosal polymorphonuclear leukocyte to infiltrate. Dramatic increase in IL-8 gene transcription points to this chemokine as the major molecule orchestrating mucosal inflammation in shigellosis.

Received for publication, April 10, 2003 , and in revised form, June 12, 2003.

^* This work was supported by a Program from the French Ministry of Research and Advanced Technologies and by a special APEX fund from INSERM. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplementary data showing complete list of genes whose transcription was up-regulated.

^¶ To whom correspondence shoule be addressed. Tel.: 33-1-40-61-30-95; Fax: 33-1-45-68-89-53; E-mail: psanson{at}pasteur.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. L. Mumy, J. D. Bien, M. A. Pazos, K. Gronert, B. P. Hurley, and B. A. McCormick Distinct Isoforms of Phospholipase A2 Mediate the Ability of Salmonella enterica Serotype Typhimurium and Shigella flexneri To Induce the Transepithelial Migration of Neutrophils Infect. Immun., August 1, 2008; 76(8): 3614 - 3627. [Abstract] [Full Text] [PDF]

				B. Sperandio, B. Regnault, J. Guo, Z. Zhang, S. L. Stanley Jr., P. J. Sansonetti, and T. Pedron Virulent Shigella flexneri subverts the host innate immune response through manipulation of antimicrobial peptide gene expression J. Exp. Med., May 12, 2008; 205(5): 1121 - 1132. [Abstract] [Full Text] [PDF]

				M.-I. Fernandez, B. Regnault, C. Mulet, M. Tanguy, P. Jay, P. J. Sansonetti, and T. Pedron Maturation of Paneth Cells Induces the Refractory State of Newborn Mice to Shigella Infection J. Immunol., April 1, 2008; 180(7): 4924 - 4930. [Abstract] [Full Text] [PDF]

				G. N. Schroeder and H. Hilbi Molecular Pathogenesis of Shigella spp.: Controlling Host Cell Signaling, Invasion, and Death by Type III Secretion Clin. Microbiol. Rev., January 1, 2008; 21(1): 134 - 156. [Abstract] [Full Text] [PDF]

				I. Treede, A. Braun, R. Sparla, M. Kuhnel, T. Giese, J. R. Turner, E. Anes, H. Kulaksiz, J. Fullekrug, W. Stremmel, et al. Anti-inflammatory Effects of Phosphatidylcholine J. Biol. Chem., September 14, 2007; 282(37): 27155 - 27164. [Abstract] [Full Text] [PDF]

				M. A. Hamon, E. Batsche, B. Regnault, T. N. Tham, S. Seveau, C. Muchardt, and P. Cossart Histone modifications induced by a family of bacterial toxins PNAS, August 14, 2007; 104(33): 13467 - 13472. [Abstract] [Full Text] [PDF]

				W. Rodenburg, I. M. J. Bovee-Oudenhoven, E. Kramer, R. van der Meer, and J. Keijer Gene expression response of the rat small intestine following oral Salmonella infection Physiol Genomics, July 18, 2007; 30(2): 123 - 133. [Abstract] [Full Text] [PDF]

				R. Shaykhiev and R. Bals Interactions between epithelial cells and leukocytes in immunity and tissue homeostasis J. Leukoc. Biol., July 1, 2007; 82(1): 1 - 15. [Abstract] [Full Text] [PDF]

				C. S. Clark and A. T. Maurelli Shigella flexneri Inhibits Staurosporine-Induced Apoptosis in Epithelial Cells Infect. Immun., May 1, 2007; 75(5): 2531 - 2539. [Abstract] [Full Text] [PDF]

				M.-T. Tien, S. E. Girardin, B. Regnault, L. Le Bourhis, M.-A. Dillies, J.-Y. Coppee, R. Bourdet-Sicard, P. J. Sansonetti, and T. Pedron Anti-Inflammatory Effect of Lactobacillus casei on Shigella-Infected Human Intestinal Epithelial Cells J. Immunol., January 15, 2006; 176(2): 1228 - 1237. [Abstract] [Full Text] [PDF]

				K. Roxstrom-Lindquist, E. Ringqvist, D. Palm, and S. Svard Giardia lamblia-Induced Changes in Gene Expression in Differentiated Caco-2 Human Intestinal Epithelial Cells Infect. Immun., December 1, 2005; 73(12): 8204 - 8208. [Abstract] [Full Text] [PDF]

				D. W. Kim, G. Lenzen, A.-L. Page, P. Legrain, P. J. Sansonetti, and C. Parsot The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes PNAS, September 27, 2005; 102(39): 14046 - 14051. [Abstract] [Full Text] [PDF]

				C. Moreilhon, D. Gras, C. Hologne, O. Bajolet, F. Cottrez, V. Magnone, M. Merten, H. Groux, E. Puchelle, and P. Barbry Live Staphylococcus aureus and bacterial soluble factors induce different transcriptional responses in human airway cells Physiol Genomics, February 10, 2005; 20(3): 244 - 255. [Abstract] [Full Text] [PDF]

				T. Le Gall, P. Darlu, P. Escobar-Paramo, B. Picard, and E. Denamur Selection-driven transcriptome polymorphism in Escherichia coli/Shigella species Genome Res., February 1, 2005; 15(2): 260 - 268. [Abstract] [Full Text] [PDF]

				M. Singer and P. J. Sansonetti IL-8 Is a Key Chemokine Regulating Neutrophil Recruitment in a New Mouse Model of Shigella-Induced Colitis J. Immunol., September 15, 2004; 173(6): 4197 - 4206. [Abstract] [Full Text] [PDF]

				P. Munro, G. Flatau, A. Doye, L. Boyer, O. Oregioni, J.-L. Mege, L. Landraud, and E. Lemichez Activation and Proteasomal Degradation of Rho GTPases by Cytotoxic Necrotizing Factor-1 Elicit a Controlled Inflammatory Response J. Biol. Chem., August 20, 2004; 279(34): 35849 - 35857. [Abstract] [Full Text] [PDF]