HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 38, 36848-36858, September 19, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/38/36848    most recent M301540200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, W. Articles by Law, P.-Y. Search for Related Content PubMed PubMed Citation Articles by Wang, W. Articles by Law, P.-Y. Social Bookmarking                      What's this?

The Intracellular Trafficking of Opioid Receptors Directed by Carboxyl Tail and a Di-leucine Motif in Neuro2A Cells^*

Wei Wang, Horace H. Loh  and Ping-Yee Law  

From the Department of Pharmacology, Medical School, University of Minnesota, Minneapolis, Minnesota 55455-0217

The µ- and -opioid receptors (MOR and DOR) differ significantly in their intracellular trafficking. MORs recycle back to the cell surface upon agonist treatment, whereas most internalized DORs are targeted to lysosomes for degradation. By exchanging the carboxyl tail domains of MOR and DOR and expressing the receptor chimeras in mouse neuroblastoma Neuro2A cells, it could be demonstrated that the carboxyl tail domain is not the sole determinant in directing the intracellular trafficking in these Neuro2A cells. Deletion of the dileucine motif (Leu²⁴⁵-Leu²⁴⁶) within the third intracellular loop of DOR or the mutation of Leu²⁴⁵ to Met slowed the lysosomal targeting of these -opioid receptors. Meanwhile the mutation of Met²⁶⁴ to Leu increased the rate of agonist-induced receptor internalization and the lysosomal targeting of the wild type and the -opioid receptor carboxyl tail chimera of the µ-opioid receptor. These studies suggest interplay between a di-leucine motif and the carboxyl tail in the lysosomal targeting of the receptor.

Received for publication, February 13, 2003 , and in revised form, June 3, 2003.

^* This work was supported in parts by National Institutes of Health Grants DA07339 and DA11806. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of National Institutes of Health Grant K05 DA70544.

Recipient of National Institutes of Health Grant K05 DA00513. To whom correspondence should be addressed: Dept. of Pharmacology, Medical School, University of Minnesota, 6-120 Jackson Hall, 321 Church St. S.E., Minneapolis, MN 55455-0217. Tel.: 612-626-6539; Fax: 612-625-8408; E-mail: lawxx001{at}umn.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Qiu, H. H. Loh, and P.-Y. Law Phosphorylation of the {delta}-Opioid Receptor Regulates Its beta-Arrestins Selectivity and Subsequent Receptor Internalization and Adenylyl Cyclase Desensitization J. Biol. Chem., August 3, 2007; 282(31): 22315 - 22323. [Abstract] [Full Text] [PDF]

				D.-F. Wu, T. Koch, Y.-J. Liang, R. Stumm, S. Schulz, H. Schroder, and V. Hollt Membrane Glycoprotein M6a Interacts with the {micro}-Opioid Receptor and Facilitates Receptor Endocytosis and Recycling J. Biol. Chem., July 27, 2007; 282(30): 22239 - 22247. [Abstract] [Full Text] [PDF]

				K. Y. Song, C. K. Hwang, C. S. Kim, H. S. Choi, P.-Y. Law, L.-N. Wei, and H. H. Loh Translational repression of mouse mu opioid receptor expression via leaky scanning Nucleic Acids Res., March 12, 2007; 35(5): 1501 - 1513. [Abstract] [Full Text] [PDF]

				C. Dong and G. Wu Regulation of Anterograde Transport of {alpha}2-Adrenergic Receptors by the N Termini at Multiple Intracellular Compartments J. Biol. Chem., December 15, 2006; 281(50): 38543 - 38554. [Abstract] [Full Text] [PDF]

				X.-Q. Ren, S.-B. Cheng, M. W. Treuil, J. Mukherjee, J. Rao, K. H. Braunewell, J. M. Lindstrom, and R. Anand Structural Determinants of {alpha}4{beta}2 Nicotinic Acetylcholine Receptor Trafficking J. Neurosci., July 13, 2005; 25(28): 6676 - 6686. [Abstract] [Full Text] [PDF]

				P.-Y. Law, L. J. Erickson-Herbrandson, Q. Q. Zha, J. Solberg, J. Chu, A. Sarre, and H. H. Loh Heterodimerization of {micro}- and {delta}-Opioid Receptors Occurs at the Cell Surface Only and Requires Receptor-G Protein Interactions J. Biol. Chem., March 25, 2005; 280(12): 11152 - 11164. [Abstract] [Full Text] [PDF]

				D. Ott, Y. Neldner, R. Cebe, I. Dodevski, and A. Pluckthun Engineering and functional immobilization of opioid receptors Protein Eng. Des. Sel., March 1, 2005; 18(3): 153 - 160. [Abstract] [Full Text] [PDF]

				B. M. Cox Agonists at {micro}-Opioid Receptors Spin the Wheels to Keep the Action Going Mol. Pharmacol., January 1, 2005; 67(1): 12 - 14. [Abstract] [Full Text] [PDF]

				G. A. Vargas and M. von Zastrow Identification of a Novel Endocytic Recycling Signal in the D1 Dopamine Receptor J. Biol. Chem., September 3, 2004; 279(36): 37461 - 37469. [Abstract] [Full Text] [PDF]

				N. R. Farid and M. W. Szkudlinski Minireview: Structural and Functional Evolution of the Thyrotropin Receptor Endocrinology, September 1, 2004; 145(9): 4048 - 4057. [Abstract] [Full Text] [PDF]

				C. Galet, T. Hirakawa, and M. Ascoli The Postendocytotic Trafficking of the Human Lutropin Receptor Is Mediated by a Transferable Motif Consisting of the C-Terminal Cysteine and an Upstream Leucine Mol. Endocrinol., February 1, 2004; 18(2): 434 - 446. [Abstract] [Full Text] [PDF]