HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 39, 37672-37680, September 26, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/39/37672    most recent M304664200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fontaine, C. Articles by Staels, B. Search for Related Content PubMed PubMed Citation Articles by Fontaine, C. Articles by Staels, B. Social Bookmarking                      What's this?

The Orphan Nuclear Receptor Rev-Erb Is a Peroxisome Proliferator-activated Receptor (PPAR) Target Gene and Promotes PPAR-induced Adipocyte Differentiation^*

Coralie Fontaine  , Guillaume Dubois  , Yannick Duguay  , Torben Helledie ¶, Ngoc Vu-Dac , Philippe Gervois , Fabrice Soncin ||, Susanne Mandrup ¶, Jean-Charles Fruchart , Jamila Fruchart-Najib  and Bart Staels  **

From the UR545 INSERM, Département d'Athérosclérose, 1, rue Calmette, Institut Pasteur de Lille, 59019 Lille, France, and Faculté de Pharmacie, Université de Lille II, 59006 Lille, France, the ^||Institut de Biologie de Lille, 1 rue Calmette, 59021 Lille, France, and the ^¶Department of Biochemistry and Molecular Biology, University of Southern Odense 5230, Denmark

Rev-Erb (NR1D1) is an orphan nuclear receptor encoded on the opposite strand of the thyroid receptor gene. Rev-Erb mRNA is induced during adipocyte differentiation of 3T3-L1 cells, and its expression is abundant in rat adipose tissue. Peroxisome proliferator-activated receptor (PPAR) (NR1C3) is a nuclear receptor controlling adipocyte differentiation and insulin sensitivity. Here we show that Rev-Erb expression is induced by PPAR activation with rosiglitazone in rat epididymal and perirenal adipose tissues in vivo as well as in 3T3-L1 adipocytes in vitro. Furthermore, activated PPAR induces Rev-Erb promoter activity by binding to the direct repeat (DR)-2 response element Rev-DR2. Mutations of the 5' or 3' half-sites of the response element totally abrogated PPAR binding and transcriptional activation, identifying this site as a novel type of functional PPAR response element. Finally, ectopic expression of Rev-Erb in 3T3-L1 preadipocytes potentiated adipocyte differentiation induced by the PPAR ligand rosiglitazone. These results identify Rev-Erb as a target gene of PPAR in adipose tissue and demonstrate a role for this nuclear receptor as a promoter of adipocyte differentiation.

Received for publication, May 5, 2003

^* This work was supported by grants from Fonds Européens de Développement Régional, Conseil Régional Région Nord/Pas-de-Calais Genopole Project 01360124 and grants from the Leducq Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

These authors contributed equally to this work.

^** To whom correspondence should be addressed: UR545 INSERM, Département d'Athérosclérose, Institut Pasteur, 1 rue Calmette, 59019 Lille, France. Tel.: 33-3-20-87-73-88; Fax: 33-3-20-87-71-98; E-mail: Bart.Staels{at}pasteur-lille.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Hummasti and P. Tontonoz Adopting New Orphans into the Family of Metabolic Regulators Mol. Endocrinol., August 1, 2008; 22(8): 1743 - 1753. [Abstract] [Full Text] [PDF]

				C. Fontaine, E. Rigamonti, B. Pourcet, H. Duez, C. Duhem, J.-C. Fruchart, G. Chinetti-Gbaguidi, and B. Staels The Nuclear Receptor Rev-erb{alpha} Is a Liver X Receptor (LXR) Target Gene Driving a Negative Feedback Loop on Select LXR-Induced Pathways in Human Macrophages Mol. Endocrinol., August 1, 2008; 22(8): 1797 - 1811. [Abstract] [Full Text] [PDF]

				T. P. Burris Nuclear Hormone Receptors for Heme: REV-ERB{alpha} and REV-ERB{beta} Are Ligand-Regulated Components of the Mammalian Clock Mol. Endocrinol., July 1, 2008; 22(7): 1509 - 1520. [Abstract] [Full Text] [PDF]

				J. Wang and M. A. Lazar Bifunctional Role of Rev-erb{alpha} in Adipocyte Differentiation Mol. Cell. Biol., April 1, 2008; 28(7): 2213 - 2220. [Abstract] [Full Text] [PDF]

				J. Tordjman, S. Leroyer, G. Chauvet, J. Quette, C. Chauvet, C. Tomkiewicz, C. Chapron, R. Barouki, C. Forest, M. Aggerbeck, et al. Cytosolic Aspartate Aminotransferase, a New Partner in Adipocyte Glyceroneogenesis and an Atypical Target of Thiazolidinedione J. Biol. Chem., August 10, 2007; 282(32): 23591 - 23602. [Abstract] [Full Text] [PDF]

				A. Anzulovich, A. Mir, M. Brewer, G. Ferreyra, C. Vinson, and R. Baler Elovl3: a model gene to dissect homeostatic links between the circadian clock and nutritional status J. Lipid Res., December 1, 2006; 47(12): 2690 - 2700. [Abstract] [Full Text] [PDF]

				M. Schupp, M. Clemenz, R. Gineste, H. Witt, J. Janke, S. Helleboid, N. Hennuyer, P. Ruiz, T. Unger, B. Staels, et al. Molecular Characterization of New Selective Peroxisome Proliferator-Activated Receptor {gamma} Modulators With Angiotensin Receptor Blocking Activity Diabetes, December 1, 2005; 54(12): 3442 - 3452. [Abstract] [Full Text] [PDF]

				H. Ando, H. Yanagihara, Y. Hayashi, Y. Obi, S. Tsuruoka, T. Takamura, S. Kaneko, and A. Fujimura Rhythmic Messenger Ribonucleic Acid Expression of Clock Genes and Adipocytokines in Mouse Visceral Adipose Tissue Endocrinology, December 1, 2005; 146(12): 5631 - 5636. [Abstract] [Full Text] [PDF]

				S. Shimba, N. Ishii, Y. Ohta, T. Ohno, Y. Watabe, M. Hayashi, T. Wada, T. Aoyagi, and M. Tezuka Brain and muscle Arnt-like protein-1 (BMAL1), a component of the molecular clock, regulates adipogenesis PNAS, August 23, 2005; 102(34): 12071 - 12076. [Abstract] [Full Text] [PDF]

				A. P. Kumar, F. J. Piedrafita, and W. F. Reynolds Peroxisome Proliferator-activated Receptor {gamma} Ligands Regulate Myeloperoxidase Expression in Macrophages by an Estrogen-dependent Mechanism Involving the -463GA Promoter Polymorphism J. Biol. Chem., February 27, 2004; 279(9): 8300 - 8315. [Abstract] [Full Text] [PDF]