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J. Biol. Chem., Vol. 278, Issue 39, 38068-38075, September 26, 2003

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Interaction between the Varicella Zoster Virus IE62 Major Transactivator and Cellular Transcription Factor Sp1^*

Hua Peng, Hongying He, John Hay and William T. Ruyechan 

From the Department of Microbiology and Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, Buffalo, New York 14214

The varicella zoster virus (VZV) IE62 protein is involved in the activation of expression of all three kinetic classes of VZV proteins. Analysis of the viral promoter for VZV glycoprotein I has shown that the cellular factor Sp1 is involved in or required for the observed IE62 mediated activation. Co-immunoprecipitation experiments show that the two proteins are present in a complex in VZV-infected cells. Protein affinity pull-down assays using recombinant proteins showed that IE62 and Sp1 interact in the absence of any other viral and cellular proteins. Mapping studies using GST-fusion proteins containing truncations of IE62 and Sp1 have delimited the interacting regions to amino acids 612–778 in Sp1 and amino acids 226–299 in IE62. The region identified in Sp1 is involved in DNA-binding, synergistic Sp1 activation, and Sp1 interaction with cellular transcription factors. The interacting region identified in IE62 overlaps with or borders on sites involved in interactions with the VZV IE4 protein and the cellular factors TBP and TFIIB. Assays using wild-type and mutant promoter elements indicate that Sp1 is involved in recruitment of IE62 to the gI promoter and IE62 enhances Sp1 and TBP binding.

Received for publication, March 4, 2003 , and in revised form, June 25, 2003.

^* This work was supported by Grant AI18449 from the NIAID, National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Microbiology, 151 Biomedical Research Bldg., University at Buffalo, Buffalo, NY 14214. Tel.: 716-829-2312; Fax: 716-829-2376; E-mail: ruyechan{at}buffalo.edu.

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