| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 39, 38068-38075, September 26, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Microbiology and Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, Buffalo, New York 14214
The varicella zoster virus (VZV) IE62 protein is involved in the activation of expression of all three kinetic classes of VZV proteins. Analysis of the viral promoter for VZV glycoprotein I has shown that the cellular factor Sp1 is involved in or required for the observed IE62 mediated activation. Co-immunoprecipitation experiments show that the two proteins are present in a complex in VZV-infected cells. Protein affinity pull-down assays using recombinant proteins showed that IE62 and Sp1 interact in the absence of any other viral and cellular proteins. Mapping studies using GST-fusion proteins containing truncations of IE62 and Sp1 have delimited the interacting regions to amino acids 612–778 in Sp1 and amino acids 226–299 in IE62. The region identified in Sp1 is involved in DNA-binding, synergistic Sp1 activation, and Sp1 interaction with cellular transcription factors. The interacting region identified in IE62 overlaps with or borders on sites involved in interactions with the VZV IE4 protein and the cellular factors TBP and TFIIB. Assays using wild-type and mutant promoter elements indicate that Sp1 is involved in recruitment of IE62 to the gI promoter and IE62 enhances Sp1 and TBP binding.
Received for publication, March 4, 2003 , and in revised form, June 25, 2003.
* This work was supported by Grant AI18449 from the NIAID, National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Microbiology, 151 Biomedical Research Bldg., University at Buffalo, Buffalo, NY 14214. Tel.: 716-829-2312; Fax: 716-829-2376; E-mail: ruyechan{at}buffalo.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Berarducci, M. Sommer, L. Zerboni, J. Rajamani, and A. M. Arvin Cellular and Viral Factors Regulate the Varicella-Zoster Virus gE Promoter during Viral Replication J. Virol., October 1, 2007; 81(19): 10258 - 10267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Narayanan, W. T. Ruyechan, and T. M. Kristie The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection PNAS, June 26, 2007; 104(26): 10835 - 10840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Che, B. Berarducci, M. Sommer, W. T. Ruyechan, and A. M. Arvin The Ubiquitous Cellular Transcriptional Factor USF Targets the Varicella-Zoster Virus Open Reading Frame 10 Promoter and Determines Virulence in Human Skin Xenografts in SCIDhu Mice In Vivo J. Virol., April 1, 2007; 81(7): 3229 - 3239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cilloniz, W. Jackson, C. Grose, D. Czechowski, J. Hay, and W. T. Ruyechan The Varicella-Zoster Virus (VZV) ORF9 Protein Interacts with the IE62 Major VZV Transactivator J. Virol., January 15, 2007; 81(2): 761 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Zapata, M. Nakatsugawa, and J. F. Moffat Varicella-Zoster Virus Infection of Human Fibroblast Cells Activates the c-Jun N-Terminal Kinase Pathway J. Virol., January 15, 2007; 81(2): 977 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yang, H. Peng, J. Hay, and W. T. Ruyechan Promoter Activation by the Varicella-Zoster Virus Major Transactivator IE62 and the Cellular Transcription Factor USF. J. Virol., August 1, 2006; 80(15): 7339 - 7353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Eisfeld, S. E. Turse, S. A. Jackson, E. C. Lerner, and P. R. Kinchington Phosphorylation of the Varicella-Zoster Virus (VZV) Major Transcriptional Regulatory Protein IE62 by the VZV Open Reading Frame 66 Protein Kinase J. Virol., February 15, 2006; 80(4): 1710 - 1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fang, E. K. Stachowiak, S. M. Dunham-Ems, I. Klejbor, and M. K. Stachowiak Control of CREB-binding Protein Signaling by Nuclear Fibroblast Growth Factor Receptor-1: A NOVEL MECHANISM OF GENE REGULATION J. Biol. Chem., August 5, 2005; 280(31): 28451 - 28462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Narayanan, M. L. Nogueira, W. T. Ruyechan, and T. M. Kristie Combinatorial Transcription of Herpes Simplex Virus and Varicella Zoster Virus Immediate Early Genes Is Strictly Determined by the Cellular Coactivator HCF-1 J. Biol. Chem., January 14, 2005; 280(2): 1369 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rahaus, N. Desloges, and M. H. Wolff Replication of varicella-zoster virus is influenced by the levels of JNK/SAPK and p38/MAPK activation J. Gen. Virol., December 1, 2004; 85(12): 3529 - 3540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yang, J. Hay, and W. T. Ruyechan The DNA Element Controlling Expression of the Varicella-Zoster Virus Open Reading Frame 28 and 29 Genes Consists of Two Divergent Unidirectional Promoters Which Have a Common USF Site J. Virol., October 15, 2004; 78(20): 10939 - 10952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grose, S. Tyler, G. Peters, J. Hiebert, G. M. Stephens, W. T. Ruyechan, W. Jackson, J. Storlie, and G. A. Tipples Complete DNA Sequence Analyses of the First Two Varicella-Zoster Virus Glycoprotein E (D150N) Mutant Viruses Found in North America: Evolution of Genotypes with an Accelerated Cell Spread Phenotype J. Virol., July 1, 2004; 78(13): 6799 - 6807. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
