Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M209622200 on November 4, 2002

J. Biol. Chem., Vol. 278, Issue 4, 2219-2227, January 24, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/4/2219    most recent
M209622200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Varin, A.
Right arrow Articles by Aggarwal, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Varin, A.
Right arrow Articles by Aggarwal, B. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Exogenous Nef Protein Activates NF-kappa B, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells
ROLE IN AIDS PATHOGENESIS*

Audrey VarinDagger , Sunil K. Manna§, Vincent Quivy||, Anne-Zélie DecrionDagger , Carine Van Lint**, Georges HerbeinDagger , and Bharat B. Aggarwal§Dagger Dagger

From the Dagger  Department of Virology and Institut d'Etude et de Transfert de Gènes, Franche-Comté University, F-25030 Besançon, France, the  Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), Chimie Biologique, 6041 Gosselies, Belgium, and the § Cytokine Research Section, Department of Bioimmunotherapy, Cytokine Research Section, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030

The human immunodeficiency virus (HIV) Nef protein plays a critical role in AIDS pathogenesis by enhancing replication and survival of the virus within infected cells and by facilitating its spread in vivo. Most of the data obtained so far have been in experiments with endogenous Nef protein, so far overlooking the effects of exogenous soluble Nef protein. We used recombinant exogenous Nef proteins to activate nuclear transcription factors NF-kappa B and AP-1 in the promonocytic cell line U937. Exogenous SIV and HIV-1 Nef proteins activated NF-kappa B and AP-1 in a dose- and time-dependent manner. Activation of NF-kappa B by exogenous Nef was concomitant to the degradation of the inhibitor of NF-kappa B, Ikappa Balpha . In agreement with increased AP-1 activation, a time- and dose-dependent increase in JNK activation was observed following treatment of U937 cells with exogenous Nef. Since exogenous Nef activates the transcription factors NF-kappa B and AP-1, which bind to the HIV-1 long terminal repeat (LTR), we investigated the effect of exogenous Nef on HIV-1 replication. We observed that exogenous Nef stimulated HIV-1 LTR via NF-kappa B activation in U937 cells and enhanced viral replication in the chronically infected promonocytic cells U1. Therefore, our results suggest that exogenous Nef could fuel the progression of the disease via stimulation of HIV-1 provirus present in such cellular reservoirs as mononuclear phagocytes in HIV-infected patients.

* This research was funded by The Clayton Foundation for Research (to B. B. A.), by grants from the Franche-Comté University, France (to G. H.), and from the Fonds National de la Recherche Scientifique (FNRS, Belgium), the Télévie-Program, the Université Libre de Bruxelles (ULB), the Internationale Brachet Stiftung, the CGRI-INSERM cooperation, the Région Wallonne-Commission Européenne FEDER, the Agence Nationale de Recherches sur le SIDA (ANRS, France), the Theyskens-Mineur Foundation (to C. V. L.), the Biotechnology and Biological Sciences Research Council (Grant 24/C12902), and the European Union Fifth Framework (Grant QLK2-CT-2000-01630).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| Aspirant of the FNRS.

** Maître de Recherches of the FNRS.

Dagger Dagger To whom correspondence should be addressed: Cytokine Research Section; Dept. of Bioimmunotherapy, The University of Texas, M. D. Anderson Cancer Center; Houston, TX 77030. Tel.: 713-794-1817; Fax: 713-794-1613; E-mail: aggarwal@mdanderson.org.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. A. Khan, A. Coaquette, C. Davrinche, and G. Herbein
Bcl-3-Regulated Transcription from Major Immediate-Early Promoter of Human Cytomegalovirus in Monocyte-Derived Macrophages
J. Immunol., June 15, 2009; 182(12): 7784 - 7794.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Ma, S. Mishra, N. Gajanayaka, J. B. Angel, and A. Kumar
HIV-1 Nef Inhibits Lipopolysaccharide-induced IL-12p40 Expression by Inhibiting JNK-activated NF{kappa}B in Human Monocytic Cells
J. Biol. Chem., March 20, 2009; 284(12): 7578 - 7587.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Mangino, Z. A. Percario, G. Fiorucci, G. Vaccari, S. Manrique, G. Romeo, M. Federico, M. Geyer, and E. Affabris
In Vitro Treatment of Human Monocytes/Macrophages with Myristoylated Recombinant Nef of Human Immunodeficiency Virus Type 1 Leads to the Activation of Mitogen-Activated Protein Kinases, I{kappa}B Kinases, and Interferon Regulatory Factor 3 and to the Release of Beta Interferon
J. Virol., March 15, 2007; 81(6): 2777 - 2791.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Rizzi, M. P. Crippa, R. E. Jeeninga, B. Berkhout, F. Blasi, G. Poli, and M. Alfano
Pertussis Toxin B-Oligomer Suppresses IL-6 Induced HIV-1 and Chemokine Expression in Chronically Infected U1 Cells via Inhibition of Activator Protein 1
J. Immunol., January 15, 2006; 176(2): 999 - 1006.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Varin, A.-Z. Decrion, E. Sabbah, V. Quivy, J. Sire, C. Van Lint, B. P. Roques, B. B. Aggarwal, and G. Herbein
Synthetic Vpr Protein Activates Activator Protein-1, c-Jun N-terminal Kinase, and NF-{kappa}B and Stimulates HIV-1 Transcription in Promonocytic Cells and Primary Macrophages
J. Biol. Chem., December 30, 2005; 280(52): 42557 - 42567.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. A. Acheampong, Z. Parveen, L. W. Muthoga, V. Wasmuth-Peroud, M. Kalayeh, A. Bashir, R. Diecidue, M. Mukhtar, and R. J. Pomerantz
Molecular Interactions of Human Immunodeficiency Virus Type 1 with Primary Human Oral Keratinocytes
J. Virol., July 1, 2005; 79(13): 8440 - 8453.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. B. Lee, J. Park, J. U. Jung, and J. Chung
Nef induces apoptosis by activating JNK signaling pathway and inhibits NF-{kappa}B-dependent immune responses in Drosophila
J. Cell Sci., May 1, 2005; 118(9): 1851 - 1859.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
U. Mahlknecht, J. Will, A. Varin, D. Hoelzer, and G. Herbein
Histone Deacetylase 3, a Class I Histone Deacetylase, Suppresses MAPK11-Mediated Activating Transcription Factor-2 Activation and Represses TNF Gene Expression
J. Immunol., September 15, 2004; 173(6): 3979 - 3990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Heckmann, C. Waltzinger, P. Jolicoeur, M. Dreano, M. H. Kosco-Vilbois, and Y. Sagot
IKK2 Inhibitor Alleviates Kidney and Wasting Diseases in a Murine Model of Human AIDS
Am. J. Pathol., April 1, 2004; 164(4): 1253 - 1262.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. G. QUARANTA, B. MATTIOLI, F. SPADARO, E. STRAFACE, L. GIORDANI, C. RAMONI, W. MALORNI, and M. VIORA
HIV-1 Nef triggers Vav-mediated signaling pathway leading to functional and morphological differentiation of dendritic cells
FASEB J, November 1, 2003; 17(14): 2025 - 2036.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
O. Rohr, C. Marban, D. Aunis, and E. Schaeffer
Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells
J. Leukoc. Biol., November 1, 2003; 74(5): 736 - 749.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Z. Percario, E. Olivetta, G. Fiorucci, G. Mangino, S. Peretti, G. Romeo, E. Affabris, and M. Federico
Human immunodeficiency virus type 1 (HIV-1) Nef activates STAT3 in primary human monocyte/macrophages through the release of soluble factors: involvement of Nef domains interacting with the cell endocytotic machinery
J. Leukoc. Biol., November 1, 2003; 74(5): 821 - 832.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement