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J. Biol. Chem., Vol. 278, Issue 4, 2304-2308, January 24, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/4/2304    most recent M208162200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sun, P. Articles by Loh, H. H. Search for Related Content PubMed PubMed Citation Articles by Sun, P. Articles by Loh, H. H. Social Bookmarking                      What's this?

Transcriptional Regulation of Mouse -Opioid Receptor Gene
IKAROS-2 AND UPSTREAM STIMULATORY FACTOR SYNERGIZE IN TRANS-ACTIVATING MOUSE -OPIOID RECEPTOR GENE IN T CELLS^*

Ping Sun and Horace H. Loh

From the Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Considerable evidence indicates that transcription of the -opioid receptor (dor) gene is correlated with both the expression of DOR on T cells and the capacity of DOR agonists to modulate the immunological functions of the T cell. We previously reported that increased Ikaros (Ik) binding activity over an Ik-binding site at 378 to 374 (with the translation start site designated as +1) in the mouse dor promoter was required for the enhanced transcription of dor gene in phytohemagglutinin-activated EL-4 cells, a mouse T cell line that constitutively expresses DOR. In the present study, we have analyzed further the mouse dor promoter in EL-4 cells and have demonstrated that Ik-2 homodimers bind to the 378/374 Ik-binding site and exerts a position-dependent trans-activation effect on the dor promoter. Moreover, an E box (185 to 180) that binds upstream stimulatory factor is essential for the dor promoter activity in both resting and phytohemagglutinin-activated T cells. Furthermore, we have demonstrated that Ik-2 and upstream stimulatory factor synergize in trans-activating the dor promoter via the putative Ik-binding site and the E box, respectively.

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