HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 40, 38514-38521, October 3, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/40/38514    most recent M304387200v2 M304387200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by El-Azami-El-Idrissi, M. Articles by Leclerc, C. Search for Related Content PubMed PubMed Citation Articles by El-Azami-El-Idrissi, M. Articles by Leclerc, C. Social Bookmarking                      What's this?

Interaction of Bordetella pertussis Adenylate Cyclase with CD11b/CD18

ROLE OF TOXIN ACYLATION AND IDENTIFICATION OF THE MAIN INTEGRIN INTERACTION DOMAIN^*

Mohammed El-Azami-El-Idrissi , Cécile Bauche , Jirina Loucka ¶, Radim Osicka ¶, Peter Sebo ¶, Daniel Ladant  and Claude Leclerc  ||

From the Unité de Biologie des Régulations Immunitaires, INSERM E 0352, Institut Pasteur, 75724 Paris, Unité de Biochimie des Interactions Macromoléculaires, CNRS URA 2185, Institut Pasteur, 75724 Paris, France, and the ^¶Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic

Adenylate cyclase toxin (CyaA) is one of the major virulence factors produced by Bordetella pertussis, the whooping cough agent. CyaA belongs to the repeat in toxin protein family and requires a post-translational fatty acylation to form cation-selective channels in target cell membranes and to penetrate into cytosol. We have demonstrated recently that CyaA uses the _M2 integrin (CD11b/CD18) as a specific cellular receptor. Here we show that the acylation of CyaA is required for a productive and tight interaction of the toxin with cells expressing CD11b. In addition, we demonstrate that the catalytic domain is not required for binding of CyaA to CD11b and that the main integrin interacting domain of CyaA is located in its glycine/aspartate-rich repeat region. These data decipher, for the first time, the interaction of CyaA with CD11b-positive cells and open new prospects for understanding the interaction of Bordetella pertussis with innate and adaptive immune systems.

Received for publication, April 28, 2003 , and in revised form, July 24, 2003.

^* This work was supported by the Pasteur Institute, ANRS, INSERM, CNRS (URA 2185), Grant QLK2-CT-1999-00556 from the 5th Framework Program of the European Union, by Czech Grants 310/01/0934 and A502907, and by National Institutes of Health Grant 55000334 from Howard Hughes Medical Institute (to P. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed: Unité de Biologie des Régulations Immunitaires, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France. Tel.: 33 1 45 68 86 18; Fax: 33 1 45 68 85 40; E-mail: cleclerc{at}pasteur.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. R. Paccani, F. D. Molin, M. Benagiano, D. Ladant, M. M. D'Elios, C. Montecucco, and C. T. Baldari Suppression of T-Lymphocyte Activation and Chemotaxis by the Adenylate Cyclase Toxin of Bordetella pertussis Infect. Immun., July 1, 2008; 76(7): 2822 - 2832. [Abstract] [Full Text] [PDF]

				J. Morova, R. Osicka, J. Masin, and P. Sebo RTX cytotoxins recognize {beta}2 integrin receptors through N-linked oligosaccharides PNAS, April 8, 2008; 105(14): 5355 - 5360. [Abstract] [Full Text] [PDF]

				R. Fiser, J. Masin, M. Basler, J. Krusek, V. Spulakova, I. Konopasek, and P. Sebo Third Activity of Bordetella Adenylate Cyclase (AC) Toxin-Hemolysin: MEMBRANE TRANSLOCATION OF AC DOMAIN POLYPEPTIDE PROMOTES CALCIUM INFLUX INTO CD11b+ MONOCYTES INDEPENDENTLY OF THE CATALYTIC AND HEMOLYTIC ACTIVITIES J. Biol. Chem., February 2, 2007; 282(5): 2808 - 2820. [Abstract] [Full Text] [PDF]

				C. Bauche, A. Chenal, O. Knapp, C. Bodenreider, R. Benz, A. Chaffotte, and D. Ladant Structural and Functional Characterization of an Essential RTX Subdomain of Bordetella pertussis Adenylate Cyclase Toxin J. Biol. Chem., June 23, 2006; 281(25): 16914 - 16926. [Abstract] [Full Text] [PDF]

				M. Basler, J. Masin, R. Osicka, and P. Sebo Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes Infect. Immun., April 1, 2006; 74(4): 2207 - 2214. [Abstract] [Full Text] [PDF]

				S. Tartz, J. Kamanova, M. Simsova, P. Sebo, S. Bolte, V. Heussler, B. Fleischer, and T. Jacobs Immunization with a Circumsporozoite Epitope Fused to Bordetella pertussis Adenylate Cyclase in Conjunction with Cytotoxic T-Lymphocyte-Associated Antigen 4 Blockade Confers Protection against Plasmodium berghei Liver-Stage Malaria Infect. Immun., April 1, 2006; 74(4): 2277 - 2285. [Abstract] [Full Text] [PDF]

				G. M. Donato, H.-L. J. Hsia, C. S. Green, and E. L. Hewlett Adenylate Cyclase Toxin (ACT) from Bordetella hinzii: Characterization and Differences from ACT of Bordetella pertussis J. Bacteriol., November 15, 2005; 187(22): 7579 - 7588. [Abstract] [Full Text] [PDF]

				L. Mascarell, C. Fayolle, C. Bauche, D. Ladant, and C. Leclerc Induction of Neutralizing Antibodies and Th1-Polarized and CD4-Independent CD8+ T-Cell Responses following Delivery of Human Immunodeficiency Virus Type 1 Tat Protein by Recombinant Adenylate Cyclase of Bordetella pertussis J. Virol., August 1, 2005; 79(15): 9872 - 9884. [Abstract] [Full Text] [PDF]

				A. P. Boyd, P. J. Ross, H. Conroy, N. Mahon, E. C. Lavelle, and K. H. G. Mills Bordetella pertussis Adenylate Cyclase Toxin Modulates Innate and Adaptive Immune Responses: Distinct Roles for Acylation and Enzymatic Activity in Immunomodulation and Cell Death J. Immunol., July 15, 2005; 175(2): 730 - 738. [Abstract] [Full Text] [PDF]

				G. Schlecht, J. Loucka, H. Najar, P. Sebo, and C. Leclerc Antigen Targeting to CD11b Allows Efficient Presentation of CD4+ and CD8+ T Cell Epitopes and In Vivo Th1-Polarized T Cell Priming J. Immunol., November 15, 2004; 173(10): 6089 - 6097. [Abstract] [Full Text] [PDF]

				H. M. Vordermeier, M. Simsova, K. A. Wilkinson, R. J. Wilkinson, R. G. Hewinson, P. Sebo, and C. Leclerc Recognition of Mycobacterial Antigens Delivered by Genetically Detoxified Bordetella pertussis Adenylate Cyclase by T Cells from Cattle with Bovine Tuberculosis Infect. Immun., November 1, 2004; 72(11): 6255 - 6261. [Abstract] [Full Text] [PDF]