|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 40, 38991-38997, October 3, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System*
From the Research Institute for Children, Children's Hospital, Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70118
IA-1 is a novel zinc finger transcription factor with a restricted tissue distribution in the embryonic nervous system and tumors of neuroendocrine origin. The 1.7-kilobase 5'-upstream DNA sequence of the human IA-1 gene directed transgene expression predominantly in the developing nervous system including forebrain, midbrain, hindbrain, spinal cord, retina, olfactory bulb, and cerebellum, which recapitulated the expression patterns of neuroendocrine tissues and childhood brain tumors. The IA-1 promoter deletion reporter gene constructs revealed that the sequence between -426 and -65 bp containing three putative E-boxes (
361 bp) upstream of the transcription start site was sufficient to confer tissue-specific transcriptional activity. Further mutation analysis revealed that the proximal E-box (E3) closest to the start site is critical to confer transcriptional activity. Electrophoretic mobility shift assay and transient transfection studies demonstrated that the NeuroD1 and E47 heterodimer are the key transcription factors that regulate the proximal E-box of the IA-1 promoter. Therefore, we concluded that the IA-1 gene is developmentally expressed in the nervous system and the NeuroD1/E47 transcription factors up-regulate IA-1 gene expression through the proximal E-box element of the IA-1 promoter.
Received for publication, June 25, 2003 , and in revised form, July 29, 2003.
* This work was supported by Juvenile Diabetes Research Foundation Grant 1-1999-61 and NIDDK, National Institutes of Health (NIH), Grant DK61436 (to M. S. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: Laboratory of Experimental Gerontology, NIA, NIH, Baltimore, MD 21224.
To whom correspondence should be addressed: Research Institute for Children, Children's Hospital, 200 Henry Clay Ave., Research and Education Building, Rm. 2211, New Orleans, LA 70118. Tel.: 504-896-2705; Fax: 504-896-2722; E-mail: mlan{at}chnola-research.org.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Jacob, R. Storm, D. S. Castro, C. Milton, P. Pla, F. Guillemot, C. Birchmeier, and J. Briscoe Insm1 (IA-1) is an essential component of the regulatory network that specifies monoaminergic neuronal phenotypes in the vertebrate hindbrain Development, July 15, 2009; 136(14): 2477 - 2485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Lan and M. B. Breslin Structure, expression, and biological function of INSM1 transcription factor in neuroendocrine differentiation FASEB J, July 1, 2009; 23(7): 2024 - 2033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Zhang, W.-D. Liu, N. A. Saunee, M. B. Breslin, and M. S. Lan Zinc Finger Transcription Factor INSM1 Interrupts Cyclin D1 and CDK4 Binding and Induces Cell Cycle Arrest J. Biol. Chem., February 27, 2009; 284(9): 5574 - 5581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Wang, M. Muguira, W.-D. Liu, T. Zhang, C. Chen, R. Aucoin, M. B Breslin, and M. S Lan Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription J. Endocrinol., July 1, 2008; 198(1): 29 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wildner, M. S. Gierl, M. Strehle, P. Pla, and C. Birchmeier Insm1 (IA-1) is a crucial component of the transcriptional network that controls differentiation of the sympatho-adrenal lineage Development, February 1, 2008; 135(3): 473 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Gierl, N. Karoulias, H. Wende, M. Strehle, and C. Birchmeier The Zinc-finger factor Insm1 (IA-1) is essential for the development of pancreatic beta cells and intestinal endocrine cells Genes & Dev., September 1, 2006; 20(17): 2465 - 2478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. R. Bhat, N. Maddodi, C. Shashikant, and V. Setaluri Transcriptional regulation of human MAP2 gene in melanoma: role of neuronal bHLH factors and Notch1 signaling Nucleic Acids Res., August 11, 2006; 34(13): 3819 - 3832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. F. Odenwald, W. Rasband, A. Kuzin, and T. Brody EVOPRINTER, a multigenomic comparative tool for rapid identification of functionally important DNA PNAS, October 11, 2005; 102(41): 14700 - 14705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Illi, A. Scopece, S. Nanni, A. Farsetti, L. Morgante, P. Biglioli, M. C. Capogrossi, and C. Gaetano Epigenetic Histone Modification and Cardiovascular Lineage Programming in Mouse Embryonic Stem Cells Exposed to Laminar Shear Stress Circ. Res., March 18, 2005; 96(5): 501 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Kim, K. Chu, H.-J. Kim, H.-A Seong, K.-C. Park, S. Sanyal, J. Takeda, H. Ha, M. Shong, M.-J. Tsai, et al. Orphan Nuclear Receptor Small Heterodimer Partner, a Novel Corepressor for a Basic Helix-Loop-Helix Transcription Factor BETA2/NeuroD Mol. Endocrinol., April 1, 2004; 18(4): 776 - 790. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |