HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 41, 40385-40391, October 10, 2003

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 278/41/40385    most recent M301041200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gallego, J. Articles by Lever, A. M. L. Search for Related Content PubMed PubMed Citation Articles by Gallego, J. Articles by Lever, A. M. L. Social Bookmarking                      What's this?

Rev Binds Specifically to a Purine Loop in the SL1 Region of the HIV-1 Leader RNA^*,

Jose Gallego  , Jane Greatorex ¶, Hui Zhang ||, Bin Yang ||, Shyamala Arunachalam ||, Jianhua Fang ||, John Seamons ¶, Susan Lea **, Roger J. Pomerantz || and Andrew M. L. Lever ¶ 

From the Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, the ^¶University of Cambridge Department of Medicine, Addenbrooke's Hospital, Cambridge, CB2 2QQ, United Kingdom, the ^||Center for Human Virology, Division of Infectious Diseases, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-5587, and the ^**Laboratory of Molecular Biophysics, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle.

Received for publication, January 30, 2003 , and in revised form, July 1, 2003.

^* This work was supported by United States Public Health Service Grant AI43876 (to R. J. P.) and by MRC Program Grant G9805564 and the Sykes' Trust (to A. M. L. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. 1.

Present address: Medivir UK Ltd., Peterhouse Technology Park, 100 Fulbourn Rd., Cambridge CB1 9PT, UK.

To whom correspondence should be addressed. Tel.: 44-1223-336747; Fax: 44-1223-336846; E-mail: amll1{at}mole.bio.cam.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Houzet, J. C. Paillart, F. Smagulova, S. Maurel, Z. Morichaud, R. Marquet, and M. Mougel HIV controls the selective packaging of genomic, spliced viral and cellular RNAs into virions through different mechanisms Nucleic Acids Res., April 10, 2007; (2007) gkm153v1. [Abstract] [Full Text] [PDF]

				J. S. Greatorex, E. A. Palmer, R. J. Pomerantz, J. A. Dangerfield, and A. M. L. Lever Mutation of the Rev-binding loop in the human immunodeficiency virus 1 leader causes a replication defect characterized by altered RNA trafficking and packaging. J. Gen. Virol., October 1, 2006; 87(Pt 10): 3039 - 3044. [Abstract] [Full Text] [PDF]

				W. Kasprzak, E. Bindewald, and B. A. Shapiro Structural polymorphism of the HIV-1 leader region explored by computational methods Nucleic Acids Res., December 20, 2005; 33(22): 7151 - 7163. [Abstract] [Full Text] [PDF]