JBC Transcription and Nuclear Factor Monoclonals

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M306491200 on August 12, 2003

J. Biol. Chem., Vol. 278, Issue 43, 41661-41669, October 24, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/43/41661    most recent
M306491200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pradip, D.
Right arrow Articles by Durden, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pradip, D.
Right arrow Articles by Durden, D. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Rac2 Specificity in Macrophage Integrin Signaling

POTENTIAL ROLE FOR Syk KINASE*

De Pradip, Xiaodong Peng, and Donald L. Durden{ddagger}

From the Section of Hematology/Oncology, Department of Pediatrics, Biochemistry and Molecular Biology, Herman B Wells Center for Pediatric Research, Indiana University of School of Medicine, Indianapolis, Indiana 46202

Herein we report that, despite the similarity of Rac2 to Rac1 (92% amino acid identity), macrophages derived from Rac2–/– mice, which continue to express Rac1, display a marked defect in {alpha}v{beta}3/{alpha}v{beta}5 and {alpha}4{beta}1 integrin-directed migration measured on vitronectin and fibronectin fragments (FN-H296), respectively. In contrast, mouse embryo fibroblasts derived from the Rac2 knockout mice utilize Rac1 for migration via {alpha}v{beta}3/{alpha}v{beta}5 and {alpha}4{beta}1. The genetic reconstitution of bone marrow-derived macrophages (BMM{theta}) with Rac2 restores the integrin-dependent migration of Rac2-deficient macrophages on vitronectin (VN) and FN-H296. The levels of GTP-Rac2 generated upon specific integrin engagement in wild type macrophages parallels the phenotypic defect observed in Rac2-deficient macrophages; i.e. FN-H296, {alpha}4{beta}1 > VN, {alpha}v{beta}3/{alpha}v{beta}5 > FN-CH271, {alpha}5{beta}1 > intact FN. In a COS7 cell system, the expression of Syk kinase alone is sufficient to convert the {alpha}4{beta}1 migration response to Rac2 dependence. Therefore, we present the first evidence that the {alpha}4{beta}1 receptor in blood cells has evolved a Syk-Rac2 signaling axis to transmit signals required for integrin-directed migration suggesting that Syk kinase in part encodes myeloid Rac2 specificity in vivo.

Received for publication, June 18, 2003 , and in revised form, August 4, 2003.

* This work was supported by National Institutes of Health Grants CA81403 and CA94233 (to D. L. D.) and by the Riley Research Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

{ddagger} To whom correspondence should be addressed: Herman B Wells Center for Pediatric Research, Department of Pediatrics, Biochemistry and Molecular Biology, Indiana University of School of Medicine, 1044 W. Walnut St., Indianapolis, IN 46202. Tel.: 317-278-3718; Fax: 317-274-8679; E-mail: ddurden{at}iupui.edu.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
E. Arana, N. E. Harwood, and F. D. Batista
Regulation of integrin activation through the B-cell receptor
J. Cell Sci., July 15, 2008; 121(14): 2279 - 2286.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Dey, H. E. Crosswell, P. De, R. Parsons, Q. Peng, J. D. Su, and D. L. Durden
The Protein Phosphatase Activity of PTEN Regulates Src Family Kinases and Controls Glioma Migration
Cancer Res., March 15, 2008; 68(6): 1862 - 1871.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Li, J. Yan, P. De, H.-C. Chang, A. Yamauchi, K. W. Christopherson II, N. C. Paranavitana, X. Peng, C. Kim, V. Munugulavadla, et al.
Rap1a Null Mice Have Altered Myeloid Cell Functions Suggesting Distinct Roles for the Closely Related Rap1a and 1b Proteins
J. Immunol., December 15, 2007; 179(12): 8322 - 8331.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Utomo, X. Cullere, M. Glogauer, W. Swat, and T. N. Mayadas
Vav Proteins in Neutrophils Are Required for Fc{gamma}R-Mediated Signaling to Rac GTPases and Nicotinamide Adenine Dinucleotide Phosphate Oxidase Component p40(phox)
J. Immunol., November 1, 2006; 177(9): 6388 - 6397.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Kimura, K. Kawamoto, S. Teranishi, and T. Nishida
Role of rac1 in fibronectin-induced adhesion and motility of human corneal epithelial cells.
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4323 - 4329.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. P. Wheeler, C. M. Wells, S. D. Smith, F. M. Vega, R. B. Henderson, V. L. Tybulewicz, and A. J. Ridley
Rac1 and Rac2 regulate macrophage morphology but are not essential for migration
J. Cell Sci., July 1, 2006; 119(13): 2749 - 2757.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
R. Pankov, Y. Endo, S. Even-Ram, M. Araki, K. Clark, E. Cukierman, K. Matsumoto, and K. M. Yamada
A Rac switch regulates random versus directionally persistent cell migration
J. Cell Biol., August 29, 2005; 170(5): 793 - 802.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
V. Munugalavadla, J. Borneo, D. A. Ingram, and R. Kapur
p85{alpha} subunit of class IA PI-3 kinase is crucial for macrophage growth and migration
Blood, July 1, 2005; 106(1): 103 - 109.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Vedham, H. Phee, and K. M. Coggeshall
Vav Activation and Function as a Rac Guanine Nucleotide Exchange Factor in Macrophage Colony-Stimulating Factor-Induced Macrophage Chemotaxis
Mol. Cell. Biol., May 15, 2005; 25(10): 4211 - 4220.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Yamauchi, C. Kim, S. Li, C. C. Marchal, J. Towe, S. J. Atkinson, and M. C. Dinauer
Rac2-Deficient Murine Macrophages Have Selective Defects in Superoxide Production and Phagocytosis of Opsonized Particles
J. Immunol., November 15, 2004; 173(10): 5971 - 5979.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Koenigsknecht and G. Landreth
Microglial Phagocytosis of Fibrillar {beta}-Amyloid through a {beta}1 Integrin-Dependent Mechanism
J. Neurosci., November 3, 2004; 24(44): 9838 - 9846.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.