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J. Biol. Chem., Vol. 278, Issue 43, 41954-41962, October 24, 2003

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Tissue- and Stage-specific Expression of Two Lipophorin Receptor Variants with Seven and Eight Ligand-binding Repeats in the Adult Mosquito^*

Sook-Jae Seo, Hyang-Mi Cheon**, Jianxin Sun¶, Thomas W. Sappington||, and Alexander S. Raikhel**

From the Division of Life Science, College of Natural Sciences, Gyeongsang National University, Jinju 660-701, Gyeongnam, Korea, the ^¶Department of Entomology, Michigan State University, East Lansing, Michigan 48824, the ^||United States Department of Agriculture-Agricultural Research Service, Corn Insects and Crop Genetics Research, Iowa State University, Ames, Iowa 50011, and the ^**Department of Entomology, University of California, Riverside, California 92521

We identified two splice variants of lipophorin receptor (LpR) gene products specific to the mosquito fat body (AaLpRfb) and ovary (AaLpRov) with respective molecular masses of 99.3 and 128.9 kDa. Each LpR variant encodes a member of the low density lipoprotein receptor family with five characteristic domains: 1) ligand recognition, 2) epidermal growth factor precursor, 3) putative O-linked sugar, 4) single membrane-spanning domains, and 5) the cytoplasmic tail with a highly conserved internalization signal FDNPVY. Proposed phylogenetic relationships among low density lipoprotein receptor superfamily members suggest that the LpRs of insects are more closely related to vertebrate low density lipoprotein receptors and very low density lipoprotein receptor/vitellogenin receptor than to insect vitellogenin receptor/yolk protein receptors. Two mosquito LpR isoforms differ in their amino termini, the ligand-binding domains, and O-linked sugar domains, which are generated by differential splicing. Polymerase chain reaction and Southern blot hybridization analyses show that these two transcripts originated from a single gene. Significantly, the putative ligand-binding domain consists of seven and eight complement-type, cysteine-rich repeats in AaLpRfb and AaLRov, respectively. Seven cysteine-rich repeats in AaLpRfb are identical to the second through eighth repeats of AaLpRov. Previous analyses (1) have indicated that the AaLpRov transcript is present exclusively in ovarian germ-line cells, nurse cells, and oocytes throughout the previtellogenic and vitellogenic stages, with the peak at 24–30 h after blood meal, coincident with the peak of yolk protein uptake. In contrast, the fat body-specific AaLpRfb transcript expression is restricted to the postvitellogenic period, during which yolk protein production is terminated and the fat body is transformed to a storage depot of lipid, carbohydrate, and protein.

Received for publication, July 28, 2003 , and in revised form, August 13, 2003.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AY348869

^* This work was supported by National Institutes of Health Grants AI024716 and AI36959 (to A. S. R.), a grant from the Brain Korea 21 program (to S.-J. S.) and by Advanced Basic Research Laboratory Grant R 14-2002-056-01001-0 from the Korea Science Engineering Foundation (to S. J. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

These authors contributed equally to this work.

To whom reprint requests should be addressed. E-mail: alexander.raikhel{at}ucr.edu.

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