HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 43, 42234-42239, October 24, 2003

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 278/43/42234    most recent C300285200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mizukoshi, T. Articles by Masai, H. Search for Related Content PubMed PubMed Citation Articles by Mizukoshi, T. Articles by Masai, H. Social Bookmarking                      What's this?

A Critical Role of the 3' Terminus of Nascent DNA Chains in Recognition of Stalled Replication Forks^*

Toshimi Mizukoshi¶, Taku Tanaka||, Ken-ichi Arai**, Daisuke Kohda, and Hisao Masai||

From the Department of Structural Biology, Biomolecular Engineering Research Institute, Suita, Osaka 565-0874, Japan, the ^||Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan, the ^**Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

Arrest of replication forks by various internal and external threats evokes a myriad of cellular reactions, collectively known as DNA replication checkpoint responses. In bacteria, PriA is essential for restoration of stalled replication forks and recombinational repair of double-stranded DNA breaks and is a candidate sensor protein that may recognize arrested forks. Here, we report that PriA protein specifically recognizes 3' termini of arrested nascent DNA chains at model stalled replication forks in vitro. Mutations in the putative "3' terminus binding pocket" present in the N-terminal segment of PriA result in reduced binding to stalled replication fork structures and loss of its biological functions. The results suggest a mechanism by which stalled replication forks are recognized by a sensor protein for checkpoint responses.

Received for publication, June 30, 2003 , and in revised form, July 28, 2003.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Supplemental Figs. S1 and S2 and Table S1.

These authors contributed equally to this work.

^¶ Present address: Ajinomoto Co., Inc., Basical Analytical Chemistry Group, Institute of Life Sciences, 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan.

Present address: Medical Inst. of Bioregulation, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan.

To whom correspondence should be addressed: Dept. of Cell Biology, Tokyo Metropolitan Inst. of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan. Tel.: 81-3-5685-2264; Fax: 81-3-5685-2932; E-mail: hmasai{at}rinshoken.or.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. McInerney and M. O'Donnell Replisome Fate upon Encountering a Leading Strand Block and Clearance from DNA by Recombination Proteins J. Biol. Chem., August 31, 2007; 282(35): 25903 - 25916. [Abstract] [Full Text] [PDF]

				T. Tanaka, T. Mizukoshi, K. Sasaki, D. Kohda, and H. Masai Escherichia coli PriA Protein, Two Modes of DNA Binding and Activation of ATP Hydrolysis J. Biol. Chem., July 6, 2007; 282(27): 19917 - 19927. [Abstract] [Full Text] [PDF]

				T. Tanaka and H. Masai Stabilization of a Stalled Replication Fork by Concerted Actions of Two Helicases J. Biol. Chem., February 10, 2006; 281(6): 3484 - 3493. [Abstract] [Full Text] [PDF]

				Z. You and H. Masai DNA binding and helicase actions of mouse MCM4/6/7 helicase Nucleic Acids Res., May 25, 2005; 33(9): 3033 - 3047. [Abstract] [Full Text] [PDF]

				H.-W. Chen, S. H. North, and H. Nakai Properties of the PriA Helicase Domain and Its Role in Binding PriA to Specific DNA Structures J. Biol. Chem., September 10, 2004; 279(37): 38503 - 38512. [Abstract] [Full Text] [PDF]