HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 45, 44525-44534, November 7, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/45/44525    most recent M304368200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arima, H. Articles by Asai, K. Search for Related Content PubMed PubMed Citation Articles by Arima, H. Articles by Asai, K. Social Bookmarking                      What's this?

Hyperosmolar Mannitol Stimulates Expression of Aquaporins 4 and 9 through a p38 Mitogen-activated Protein Kinase-dependent Pathway in Rat Astrocytes^*

Hajime Arima¶, Naoki Yamamoto||, Kazuya Sobue, Fuminori Umenishi**, Toyohiro Tada, Hirotada Katsuya, and Kiyofumi Asai||

From the Department of Anesthesia and Critical Care, Okazaki City Hospital, 3-1 Goshoai, Koryuji-cho, Okazaki 444-8553, Japan, the Department of Anesthesiology and Medical Crisis Management, Nagoya City University, Graduate School of Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan, the ^||Department of Molecular Neurobiology, Nagoya City University, Graduate School of Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan, the ^**Division of Renal Diseases and Hypertension, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, and the Department of Pathology, Nagoya City University School of Nursing, Mizuho-ku, Nagoya 467-8601, Japan

The membrane pore proteins, aquaporins (AQPs), facilitate the osmotically driven passage of water and, in some instances, small solutes. Under hyperosmotic conditions, the expression of some AQPs changes, and some studies have shown that the expression of AQP1 and AQP5 is regulated by MAPKs. However, the mechanisms regulating the expression of AQP4 and AQP9 induced by hyperosmotic stress are poorly understood. In this study, we observed that hyperosmotic stress induced by mannitol increased the expression of AQP4 and AQP9 in cultured rat astrocytes, and intraperitoneal infusion of mannitol increased AQP4 and AQP9 in the rat brain cortex. In addition, a p38 MAPK inhibitor, but not ERK and JNK inhibitors, suppressed their expression in cultured astrocytes. AQPs play important roles in maintaining brain homeostasis. The expression of AQP4 and AQP9 in astrocytes changes after brain ischemia or traumatic injury, and some studies have shown that p38 MAPK in astrocytes is activated under similar conditions. Since mannitol is commonly used to reduce brain edema, understanding the regulation of AQPs and p38 MAPK in astrocytes under hyperosmotic conditions induced with mannitol may lead to a control of water movements and a new treatment for brain edema.

Received for publication, April 25, 2003 , and in revised form, August 14, 2003.

^* This work was supported by a grant-in-aid for Scientific Research (C), a grant-in-aid for Exploratory Research, and a grant-in-aid for Young Scientists (A and B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and a Research Grant from the Japan Brain Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Anesthesia and Critical Care, Okazaki City Hospital, 3-1 Goshoai, Koryuji-cho, Okazaki 444-8553, Japan. Tel.: 81-564-21-8111; Fax: 81-564-25-2913; E-mail: arima{at}sb.starcat.ne.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. B. Burg, J. D. Ferraris, and N. I. Dmitrieva Cellular Response to Hyperosmotic Stresses Physiol Rev, October 1, 2007; 87(4): 1441 - 1474. [Abstract] [Full Text] [PDF]

				J. G. Kim, Y. J. Son, C. H. Yun, Y. I. Kim, I. S. Nam-goong, J. H. Park, S. K. Park, S. R. Ojeda, A. V. D'Elia, G. Damante, et al. Thyroid Transcription Factor-1 Facilitates Cerebrospinal Fluid Formation by Regulating Aquaporin-1 Synthesis in the Brain J. Biol. Chem., May 18, 2007; 282(20): 14923 - 14931. [Abstract] [Full Text] [PDF]

				B. Zhou, D. K. Ann, X. Li, K.-J. Kim, H. Lin, P. Minoo, E. D. Crandall, and Z. Borok Hypertonic induction of aquaporin-5: novel role of hypoxia-inducible factor-1{alpha} Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1280 - C1290. [Abstract] [Full Text] [PDF]

				L. Chi-Wei, S.-L. Chang, and C.-F. Weng Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Regulates the Expression of PACAP in Cultured Tilapia Astrocytes Experimental Biology and Medicine, February 1, 2007; 232(2): 262 - 276. [Abstract] [Full Text] [PDF]

				N. P. Poolos, J. B. Bullis, and M. K. Roth Modulation of h-channels in hippocampal pyramidal neurons by p38 mitogen-activated protein kinase. J. Neurosci., July 26, 2006; 26(30): 7995 - 8003. [Abstract] [Full Text] [PDF]

				V. K. Sidhaye, A. D. Guler, K. S. Schweitzer, F. D'Alessio, M. J. Caterina, and L. S. King Transient receptor potential vanilloid 4 regulates aquaporin-5 abundance under hypotonic conditions PNAS, March 21, 2006; 103(12): 4747 - 4752. [Abstract] [Full Text] [PDF]

				J. Mallolas, O. Hurtado, M. Castellanos, M. Blanco, T. Sobrino, J. Serena, J. Vivancos, J. Castillo, I. Lizasoain, M. A. Moro, et al. A polymorphism in the EAAT2 promoter is associated with higher glutamate concentrations and higher frequency of progressing stroke J. Exp. Med., March 20, 2006; 203(3): 711 - 717. [Abstract] [Full Text] [PDF]

				M.-M. Trinh-Trang-Tan, J.-P. Cartron, and L. Bankir Molecular basis for the dialysis disequilibrium syndrome: altered aquaporin and urea transporter expression in the brain Nephrol. Dial. Transplant., September 1, 2005; 20(9): 1984 - 1988. [Abstract] [Full Text] [PDF]

				M. C. Papadopoulos and A. S. Verkman Aquaporin-4 Gene Disruption in Mice Reduces Brain Swelling and Mortality in Pneumococcal Meningitis J. Biol. Chem., April 8, 2005; 280(14): 13906 - 13912. [Abstract] [Full Text] [PDF]

				K. Kasono, T. Saito, T. Saito, H. Tamemoto, C. Yanagidate, S. Uchida, M. Kawakami, S. Sasaki, and S.-e Ishikawa Hypertonicity regulates the aquaporin-2 promoter independently of arginine vasopressin Nephrol. Dial. Transplant., March 1, 2005; 20(3): 509 - 515. [Abstract] [Full Text] [PDF]