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J. Biol. Chem., Vol. 278, Issue 46, 45109-45116, November 14, 2003

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Folding of Fumarase during Mitochondrial Import Determines its Dual Targeting in Yeast^*

Ehud Sass, Sharon Karniely, and Ophry Pines

From the Department of Molecular Biology, Hebrew University Medical School, Jerusalem 91120, Israel

We have previously proposed that a single translation product of the FUM1 gene encoding fumarase is distributed between the cytosol and mitochondria of Saccharomyces cerevisiae and that all fumarase translation products are targeted and processed in mitochondria before distribution. Thus, fumarase processed in mitochondria returns to the cytosol. In the current work, we (i) generated mutations throughout the coding sequence which resulted in fumarases with altered conformations that are targeted to mitochondria but have lost their ability to be distributed; (ii) showed by mass spectrometry that mature cytosolic and mitochondrial fumarase isoenzymes are identical; and (iii) showed that hsp70 chaperones in the cytosol (Ssa) and mitochondria (Ssc1) can affect fumarase distribution. The results are discussed in light of our model of targeting and distribution, which suggests that rapid folding of fumarase into an import-incompetent state provides the driving force for retrograde movement of the processed protein back to the cytosol through the translocation pore.

Received for publication, March 6, 2003 , and in revised form, August 21, 2003.

^* This research was supported by the German-Israeli Foundation for Scientific Research and Development (to W. N. and O. P.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

To whom correspondence should be addressed. Tel.: 9722-675-7203; Fax: 9722-675-8918; E-mail: ophry{at}md.huji.ac.il.

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