HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 47, 46337-46348, November 21, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/47/46337    most recent M302529200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Robbe, C. Articles by Michalski, J.-C. Search for Related Content PubMed PubMed Citation Articles by Robbe, C. Articles by Michalski, J.-C. Social Bookmarking                      What's this?

Evidence of Regio-specific Glycosylation in Human Intestinal Mucins

PRESENCE OF AN ACIDIC GRADIENT ALONG THE INTESTINAL TRACT^*

Catherine Robbe, Calliope Capon, Emmanuel Maes, Monique Rousset¶, Alain Zweibaum¶, Jean-Pierre Zanetta, and Jean-Claude Michalski||

From the Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche (UMR) CNRS/USTL 8576, IFR 118, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France, Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques et Biologiques de Lille 2, 59006 Lille Cedex, France, and ^¶UMR INSERM U505/UPMC, Institut Biomédical des Cordeliers, 15 rue de l'Ecole de Médecine, 75006 Paris, France

Mucin glycans were isolated from different regions of the normal human intestine (ileum, cecum, transverse and sigmoid colon, and rectum) of two individuals with ALe^b blood group. A systematic study of the monosaccharides and oligosaccharide alditols released by reductive -elimination from mucins was performed using gas chromatography, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy techniques. Important variations were observed in the mucin-associated oligosaccharide content with an increasing gradient of sialic acid from the ileum to the colon associated with a reverse gradient of fucose. Moreover, a comparative study of the Sda/Cad and ABH blood group determinants along the gastrointestinal tract showed the same reverse distribution in the two kinds of antigens. In addition, besides their heterogeneity, sialic acids presented considerable variations in the degree of O-acetylation in relation to glycan sialylation level. These data are discussed in view of recent concepts suggesting that the oligosaccharide composition of the gut constitutes a varied ecosystem for microorganisms that are susceptible to adapt there and possess the specific adhesion system and specific enzymes able to provide a carbohydrate nutrient.

Received for publication, March 12, 2003 , and in revised form, September 2, 2003.

^* This investigation was supported in part by the CNRS (Unité Mixte de Recherche CNRS/USTL 8576; Director: Dr. Jean-Claude Michalski) and by the Ministre de la Recherche et de l'Enseignement Supérieur. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed. Tel.: 33-3-20-43-41-46; Fax: 33-3-20-4365-55; E-mail: Jean-Claude.Michalski{at}univ-lille1.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. M Holmen Larsson, H. Karlsson, H. Sjovall, and G. C Hansson A complex, but uniform O-glycosylation of the human MUC2 mucin from colonic biopsies analyzed by nanoLC/MSn Glycobiology, July 1, 2009; 19(7): 756 - 766. [Abstract] [Full Text] [PDF]

				C. Brigham, R. Caughlan, R. Gallegos, M. B. Dallas, V. G. Godoy, and M. H. Malamy Sialic Acid (N-Acetyl Neuraminic Acid) Utilization by Bacteroides fragilis Requires a Novel N-Acetyl Mannosamine Epimerase J. Bacteriol., June 1, 2009; 191(11): 3629 - 3638. [Abstract] [Full Text] [PDF]

				G. Patsos, V. Hebbe-Viton, C. Robbe-Masselot, D. Masselot, R. S. Martin, R. Greenwood, C. Paraskeva, A. Klein, M. Graessmann, J. C. Michalski, et al. O-Glycan inhibitors generate aryl-glycans, induce apoptosis and lead to growth inhibition in colorectal cancer cell lines Glycobiology, April 1, 2009; 19(4): 382 - 398. [Abstract] [Full Text] [PDF]

				J. M. Johnsen, M. Teschke, P. Pavlidis, B. M. McGee, D. Tautz, D. Ginsburg, and J. F. Baines Selection on cis-Regulatory Variation at B4galnt2 and Its Influence on von Willebrand Factor in House Mice Mol. Biol. Evol., March 1, 2009; 26(3): 567 - 578. [Abstract] [Full Text] [PDF]

				H.-R. Wang, C.-Y. Hsieh, Y.-C. Twu, and L.-C. Yu Expression of the Human Sda -1,4-N-Acetylgalactosaminyltransferase II Gene is Dependent on the Promoter Methylation Status Glycobiology, January 1, 2008; 18(1): 104 - 113. [Abstract] [Full Text] [PDF]

				E. J. Nelson, A. Chowdhury, J. B. Harris, Y. A. Begum, F. Chowdhury, A. I. Khan, R. C. LaRocque, A. L. Bishop, E. T. Ryan, A. Camilli, et al. Complexity of rice-water stool from patients with Vibrio cholerae plays a role in the transmission of infectious diarrhea PNAS, November 27, 2007; 104(48): 19091 - 19096. [Abstract] [Full Text] [PDF]

				N. N. Nanthakumar, D. Dai, D. Meng, N. Chaudry, D. S. Newburg, and W. A. Walker Regulation of intestinal ontogeny: effect of glucocorticoids and luminal microbes on galactosyltransferase and trehalase induction in mice Glycobiology, March 1, 2005; 15(3): 221 - 232. [Abstract] [Full Text] [PDF]