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J. Biol. Chem., Vol. 278, Issue 47, 46878-46885, November 21, 2003

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Binding of the C-terminal Sterile Motif (SAM) Domain of Human p73 to Lipid Membranes^*

Francisco N. Barrera, José A. Poveda, José M. González-Ros¶, and José L. Neira||**

From the Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, 03202 Elche (Alicante), Spain and ^||Instituto de Biocomputación y Física de Sistemas Complejos, Universidad de Zaragoza, 50009 Zaragoza, Spain

The splice variant of p73 (p73), a homologue of the tumor suppressor p53, has close to its C terminus a sterile motif (SAM), SAMp73, that is thought to be involved in protein-protein interactions. Here, we report the lipid binding properties of this domain. Binding was assayed against zwitterionic (phosphatidylcholine) and anionic (phosphatidic acid) lipids and was studied by different biophysical techniques, namely, circular dichroism and fluorescence spectroscopies and differential scanning calorimetry. These techniques unambiguously indicate that SAMp73 binds to lipids. The binding involves protein surface attachment and partial membrane penetration, accompanied by changes in SAMp73 structure.

Received for publication, July 21, 2003 , and in revised form, September 3, 2003.

^* This work was supported by Projects GV-00-024-5 (to J. L. N.) and CITIDIB/2002/6 (to J. L. N.) from Generalitat Valenciana, BIO2000-1081 (to J. L. N.) and BFI2002-03410 (to J. M. G.-R.) from the Spanish Ministerio de Ciencia y Tecnología, and Project FIS-01/0001–02 (JLN) from the Spanish Ministerio de Sanidad y Consumo. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a predoctoral fellowship from Generalitat Valenciana.

^¶ To whom correspondence may be addressed: Instituto de Biología Molecular y Celular, Edificio Torregaitán, Universidad Miguel Hernández, Avda. del Ferrocarril s/n, 03202, Elche (Alicante), Spain. Tel.: 34-9666587571; Fax: 34-966658758; E-mail: gonzalez.ros{at}umh.es. ** To whom correspondence may be addressed: Instituto de Biología Molecular y Celular, Edificio Torregaitán, Universidad Miguel Hernández, Avda. del Ferrocarril s/n, 03202, Elche (Alicante), Spain. Tel.: 34-966658459; Fax: 34-966658758; E-mail: jlneira{at}umh.es.

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