HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 47, 47145-47155, November 21, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/47/47145    most recent M306998200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, Q. Articles by Yang, H. Search for Related Content PubMed PubMed Citation Articles by Zhang, Q. Articles by Yang, H. Social Bookmarking                      What's this?

Schizosaccharomyces pombe Cells Deficient in Triacylglycerols Synthesis Undergo Apoptosis upon Entry into the Stationary Phase^*

Qian Zhang, Hai Kee Chieu, Choon Pei Low, Shaochong Zhang, Chew Kiat Heng, and Hongyuan Yang¶

From the Departments of Biochemistry and Pediatrics, Faculty of Medicine, National University of Singapore, Singapore 119260, Republic of Singapore

Triacylglycerols (TAG) are important energy storage molecules for nearly all eukaryotic organisms. In this study, we found that two gene products (Plh1p and Dga1p) are responsible for the terminal step of TAG synthesis in the fission yeast Schizosaccharomyces pombe through two different mechanisms: Plh1p is a phospholipid diacylglycerol acyltransferase, whereas Dga1p is an acyl-CoA:diacylglycerol acyltransferase. Cells with both dga1⁺ and plh1⁺ deleted (DKO cells) lost viability upon entry into the stationary phase and demonstrated prominent apoptotic markers. Exponentially growing DKO cells also underwent dramatic apoptosis when briefly treated with diacylglycerols (DAGs) or free fatty acids. We provide strong evidence suggesting that DAG, not sphingolipids, mediates fatty acids-induced lipoapoptosis in yeast. Lastly, we show that generation of reactive oxygen species is essential to lipoapoptosis.

Received for publication, July 1, 2003 , and in revised form, September 3, 2003.

^* This work was supported by a research grant from the Academic Research Fund, National University of Singapore (NUS), by a NUS young scientist award, and by a grant from the National Medical Research Council, Singapore (all to H. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Tel.: 65-6874-7996; Fax: 65-6779-1453; E mail: bchyangr{at}nus.edu.sg.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. R. Turkish and S. L. Sturley The genetics of neutral lipid biosynthesis: an evolutionary perspective Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E19 - E27. [Abstract] [Full Text] [PDF]

				R. Guerin, G. Arseneault, S. Dumont, and L. A. Rokeach Calnexin Is Involved in Apoptosis Induced by Endoplasmic Reticulum Stress in the Fission Yeast Mol. Biol. Cell, October 1, 2008; 19(10): 4404 - 4420. [Abstract] [Full Text] [PDF]

				C. P. Low, G. Shui, L. P. Liew, S. Buttner, F. Madeo, I. W. Dawes, M. R. Wenk, and H. Yang Caspase-dependent and -independent lipotoxic cell-death pathways in fission yeast J. Cell Sci., August 15, 2008; 121(16): 2671 - 2684. [Abstract] [Full Text] [PDF]

				W. Fei, G. Shui, B. Gaeta, X. Du, L. Kuerschner, P. Li, A. J. Brown, M. R. Wenk, R. G. Parton, and H. Yang Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast J. Cell Biol., February 6, 2008; 180(3): 473 - 482. [Abstract] [Full Text] [PDF]

				W. Fei, G. Alfaro, B.-P. Muthusamy, Z. Klaassen, T. R. Graham, H. Yang, and C. T. Beh Genome-Wide Analysis of Sterol-Lipid Storage and Trafficking in Saccharomyces cerevisiae Eukaryot. Cell, February 1, 2008; 7(2): 401 - 414. [Abstract] [Full Text] [PDF]

				S. M. Turpin, G. I. Lancaster, I. Darby, M. A. Febbraio, and M. J. Watt Apoptosis in skeletal muscle myotubes is induced by ceramides and is positively related to insulin resistance Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1341 - E1350. [Abstract] [Full Text] [PDF]

				G. Ren, P. Vajjhala, J. S. Lee, B. Winsor, and A. L. Munn The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 37 - 120. [Abstract] [Full Text] [PDF]

				M. A. Marchetti, M. Weinberger, Y. Murakami, W. C. Burhans, and J. A. Huberman Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast J. Cell Sci., January 1, 2006; 119(1): 124 - 131. [Abstract] [Full Text] [PDF]

				K. Athenstaedt and G. Daum Tgl4p and Tgl5p, Two Triacylglycerol Lipases of the Yeast Saccharomyces cerevisiae Are Localized to Lipid Particles J. Biol. Chem., November 11, 2005; 280(45): 37301 - 37309. [Abstract] [Full Text] [PDF]

				A. I. Pozniakovsky, D. A. Knorre, O. V. Markova, A. A. Hyman, V. P. Skulachev, and F. F. Severin Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast J. Cell Biol., January 17, 2005; 168(2): 257 - 269. [Abstract] [Full Text] [PDF]

				S. J. Stone, H. M. Myers, S. M. Watkins, B. E. Brown, K. R. Feingold, P. M. Elias, and R. V. Farese Jr. Lipopenia and Skin Barrier Abnormalities in DGAT2-deficient Mice J. Biol. Chem., March 19, 2004; 279(12): 11767 - 11776. [Abstract] [Full Text] [PDF]