|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 5, 2969-2976, January 31, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Kolling Institute of Medical Research, University of
Sydney, Royal North Shore Hospital, St. Leonards,
New South Wales 2065, Australia
Insulin-like growth factor-binding
protein-3 (IGFBP-3) is inhibitory to the growth of many breast
cancer cells in vitro; however, a high level of expression
of IGFBP-3 in breast tumors correlates with poor prognosis, suggesting
that IGFBP-3 may be associated with growth stimulation in some breast
cancers. We have shown previously in MCF-10A breast epithelial cells
that chronic activation of Ras-p44/42 mitogen-activated protein (MAP)
kinase confers resistance to the growth-inhibitory effects of IGFBP-3
(Martin, J. L., and Baxter, R. C. (1999) J. Biol.
Chem. 274, 16407-16411). Here we show that, in the same cell
line, IGFBP-3 potentiates DNA synthesis and cell proliferation
stimulated by epidermal growth factor (EGF), a potent activator of Ras.
A mutant of IGFBP-3, which fails to translocate to the nucleus and has
reduced ability to cell-associate, similarly enhanced EGF action in
these cells. By contrast, the structurally related IGFBP-5, which
shares many functional features with IGFBP-3, was slightly inhibitory
to DNA synthesis in the presence of EGF. IGFBP-3 primes MCF-10A cells
to respond to EGF because pre-incubation caused a similar degree of EGF
potentiation as co-incubation. In IGFBP-3-primed cells, EGF-stimulated
EGF receptor phosphorylation at Tyr-1068 was increased relative to unprimed cells, as was phosphorylation and activity of p44/42 and p38
MAP kinases, but not Akt/PKB. Partial blockade of the p44/42 and p38
MAP kinase pathways abolished the potentiation by IGFBP-3 of
EGF-stimulated DNA synthesis. Collectively, these findings indicate
that IGFBP-3 enhances EGF signaling and proliferative effects in breast
epithelial cells via increased EGF receptor phosphorylation and
activation of p44/42 and p38 MAP kinase signaling pathways.
Insulin-like Growth Factor-binding Protein-3 Potentiates
Epidermal Growth Factor Action in MCF-10A Mammary Epithelial Cells
INVOLVEMENT OF p44/42 AND p38 MITOGEN-ACTIVATED PROTEIN
KINASES*
,
*
This work was supported by National Health and Medical
Research Council of Australia Grant 107244 (to J. L. M. and
R. C. B.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 61-2-9926-8486;
Fax: 61-2-9926-8484; E-mail: janetlm@med.usyd.edu.au.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. R. Dayton and M. E. White Cellular and molecular regulation of muscle growth and development in meat animals J Anim Sci, April 1, 2008; 86(14_suppl): E217 - E225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-A. Toillon, C. Lagadec, A. Page, V. Chopin, P.-E. Sautiere, J.-M. Ricort, J. Lemoine, M. Zhang, H. Hondermarck, and X. Le Bourhis Proteomics Demonstration That Normal Breast Epithelial Cells Can Induce Apoptosis of Breast Cancer Cells through Insulin-like Growth Factor-binding Protein-3 and Maspin Mol. Cell. Proteomics, July 1, 2007; 6(7): 1239 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Martin and R. C. Baxter Expression of Insulin-Like Growth Factor Binding Protein-2 by MCF-7 Breast Cancer Cells Is Regulated through the Phosphatidylinositol 3-Kinase/AKT/Mammalian Target of Rapamycin Pathway Endocrinology, May 1, 2007; 148(5): 2532 - 2541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Martin and S. Jambazov Insulin-Like Growth Factor Binding Protein-3 in Extracellular Matrix Stimulates Adhesion of Breast Epithelial Cells and Activation of p44/42 Mitogen-Activated Protein Kinase Endocrinology, September 1, 2006; 147(9): 4400 - 4409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takaoka, C. E. Smith, M. K. Mashiba, T. Okawa, C. D. Andl, W. S. El-Deiry, and H. Nakagawa EGF-mediated regulation of IGFBP-3 determines esophageal epithelial cellular response to IGF-I Am J Physiol Gastrointest Liver Physiol, February 1, 2006; 290(2): G404 - G416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Macleod, P. Mullen, J. Sewell, G. Rabiasz, S. Lawrie, E. Miller, J. F. Smyth, and S. P. Langdon Altered ErbB Receptor Signaling and Gene Expression in Cisplatin-Resistant Ovarian Cancer Cancer Res., August 1, 2005; 65(15): 6789 - 6800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Butt, K. A. Dickson, S. Jambazov, and R. C. Baxter Enhancement of Tumor Necrosis Factor-{alpha}-Induced Growth Inhibition by Insulin-Like Growth Factor-Binding Protein-5 (IGFBP-5), But Not IGFBP-3 in Human Breast Cancer Cells Endocrinology, July 1, 2005; 146(7): 3113 - 3122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C.J.P. Gielen, E. E. Hanekamp, L. J. Blok, F. J. Huikeshoven, and C. W. Burger Steroid-Modulated Proliferation of Human Endometrial Carcinoma Cell Lines: Any Role for Insulin-like Growth Factor Signaling? Reproductive Sciences, January 1, 2005; 12(1): 58 - 64. [Abstract] [PDF]
|
|
|
|
|
|
M. Takaoka, H. Harada, C. D. Andl, K. Oyama, Y. Naomoto, K. L. Dempsey, A. J. Klein-Szanto, W. S. El-Deiry, A. Grimberg, and H. Nakagawa Epidermal Growth Factor Receptor Regulates Aberrant Expression of Insulin-Like Growth Factor-Binding Protein 3 Cancer Res., November 1, 2004; 64(21): 7711 - 7723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Sivaprasad, J. Fleming, P. S. Verma, K. A. Hogan, G. Desury, and W. S. Cohick Stimulation of Insulin-Like Growth Factor (IGF) Binding Protein-3 Synthesis by IGF-I and Transforming Growth Factor-{alpha} Is Mediated by Both Phosphatidylinositol-3 Kinase and Mitogen-Activated Protein Kinase Pathways in Mammary Epithelial Cells Endocrinology, September 1, 2004; 145(9): 4213 - 4221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Butt, J. L. Martin, K. A. Dickson, F. McDougall, S. M. Firth, and R. C. Baxter Insulin-Like Growth Factor Binding Protein-3 Expression Is Associated with Growth Stimulation of T47D Human Breast Cancer Cells: The Role of Altered Epidermal Growth Factor Signaling J. Clin. Endocrinol. Metab., April 1, 2004; 89(4): 1950 - 1956. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |