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J. Biol. Chem., Vol. 278, Issue 5, 3339-3346, January 31, 2003
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From the Departments of Organisms respond to environmental stress by
adopting changes in gene expression at the transcriptional level. Rpb4,
a nonessential subunit of the core RNA polymerase II has been proposed
to play a role in non-stress-specific transcription and in the
regulation of stress response in yeast. We find that in addition to the
temperature sensitivity of the null mutant of Rpb4, diploid null
mutants are also compromised in sporulation and show morphological
changes associated with nitrogen starvation. Using whole genome
expression analysis, we report here the effects of Rpb4 on expression
of genes during normal growth and following heat shock and nutritional starvation. Our analysis shows that Rpb4 affects expression of a small
yet significant fraction of the genome in both stress and normal
conditions. We found that genes involved in galactose metabolism were
dependent on the presence of Rpb4 irrespective of the environmental
condition. Rpb4 was also found to affect the expression of several
other genes specifically in conditions of nutritional starvation. The
general defect in the absence of Rpb4 is in the expression of metabolic
genes, especially those involved in carbon metabolism and energy
generation. We report that various stresses are affected by
RPB4 and that on overexpression the stress-specific
activators can partially rescue the corresponding defects.
Whole Genome Expression Profiles of Yeast RNA
Polymerase II Core Subunit, Rpb4, in Stress and Nonstress
Conditions*,
,,,,
Microbiology and Cell Biology
and § Biochemistry, Indian Institute of Science, Bangalore,
560 012, India and the ¶ Institute for Genomics & Integrative
Biology, New Delhi, 110007 India
*
This work was supported by funds from the Council for
Scientific and Industrial Research (to P. P. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
The on-line version of this article (available at
http://www.jbc.org) contains a supplemental table
(Table SI).
To whom correspondence should be addressed. Tel.:
91-80-394-2292; Fax: 91-80-360-2697; E-mail:
pps@mcbl.iisc.ernet.in.
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