HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 50, 50128-50135, December 12, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/50/50128    most recent M309840200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, W. Articles by Dowhan, W. Search for Related Content PubMed PubMed Citation Articles by Zhang, W. Articles by Dowhan, W. Social Bookmarking                      What's this?

Reversible Topological Organization within a Polytopic Membrane Protein Is Governed by a Change in Membrane Phospholipid Composition^*

Wei Zhang, Mikhail Bogdanov, Jing Pi, A. James Pittard, and William Dowhan¶

From the Department of Biochemistry and Molecular Biology, University of Texas, Medical School and Graduate School of Biomedical Sciences, Houston, Texas 77030 and the Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia

Once inserted, transmembrane segments of polytopic membrane proteins are generally considered stably oriented due to the large free energy barrier to topological reorientation of adjacent extramembrane domains. However, the topology and function of the polytopic membrane protein lactose permease of Escherichia coli are dependent on the membrane phospholipid composition, revealing topological dynamics of transmembrane domains after stable membrane insertion (Bogdanov, M., Heacock, P. N., and Dowhan, W. (2002) EMBO J. 21, 2107–2116). In this study, we show that the high affinity phenylalanine permease PheP shares many similarities with lactose permease. PheP assembled in a mutant of E. coli lacking phosphatidylethanolamine (PE) exhibited significantly reduced active transport function and a complete inversion in topological orientation of the N terminus and adjoining transmembrane hairpin loop compared with PheP in a PE-containing strain. Introduction of PE following the assembly of PheP triggered a reorientation of the N terminus and adjacent hairpin to their native orientation associated with regain of wild-type transport function. The reversible orientation of these secondary transport proteins in response to a change in phospholipid composition might be a result of inherent conformational flexibility necessary for transport function or during protein assembly.

Received for publication, September 4, 2003 , and in revised form, October 1, 2003.

^* This work was supported in part by National Institutes of Health Grant GM20487 (to W. D.) and the Australian Research Council Large Grants Scheme (to A. J. P.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, University of Texas Medical School, 6431 Fannin St., Suite 6.200, P. O. Box 20708, Houston, TX 77030. Tel.: 713-500-6051; Fax: 713-500-0652; E-mail: William.Dowhan{at}uth.tmc.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Bogdanov, J. Xie, and W. Dowhan Lipid-Protein Interactions Drive Membrane Protein Topogenesis in Accordance with the Positive Inside Rule J. Biol. Chem., April 10, 2009; 284(15): 9637 - 9641. [Abstract] [Full Text] [PDF]

				W. Dowhan Molecular genetic approaches to defining lipid function J. Lipid Res., April 1, 2009; 50(Supplement): S305 - S310. [Abstract] [Full Text] [PDF]

				M. Wikstrom, A. A. Kelly, A. Georgiev, H. M. Eriksson, M. R. Klement, M. Bogdanov, W. Dowhan, and A. Wieslander Lipid-engineered Escherichia coli Membranes Reveal Critical Lipid Headgroup Size for Protein Function J. Biol. Chem., January 9, 2009; 284(2): 954 - 965. [Abstract] [Full Text] [PDF]

				M. Bogdanov, J. Xie, P. Heacock, and W. Dowhan To flip or not to flip: lipid-protein charge interactions are a determinant of final membrane protein topology J. Cell Biol., September 9, 2008; 182(5): 925 - 935. [Abstract] [Full Text] [PDF]

				A. Soto and G. M. Carman Regulation of the Saccharomyces cerevisiae CKI1-encoded Choline Kinase by Zinc Depletion J. Biol. Chem., April 11, 2008; 283(15): 10079 - 10088. [Abstract] [Full Text] [PDF]

				P. Hakizimana, M. Masureel, B. Gbaguidi, J.-M. Ruysschaert, and C. Govaerts Interactions between Phosphatidylethanolamine Headgroup and LmrP, a Multidrug Transporter: A CONSERVED MECHANISM FOR PROTON GRADIENT SENSING? J. Biol. Chem., April 4, 2008; 283(14): 9369 - 9376. [Abstract] [Full Text] [PDF]

				J. Xie, M. Bogdanov, P. Heacock, and W. Dowhan Phosphatidylethanolamine and Monoglucosyldiacylglycerol Are Interchangeable in Supporting Topogenesis and Function of the Polytopic Membrane Protein Lactose Permease J. Biol. Chem., July 14, 2006; 281(28): 19172 - 19178. [Abstract] [Full Text] [PDF]

				W. Zhang, H. A. Campbell, S. C. King, and W. Dowhan Phospholipids as Determinants of Membrane Protein Topology: PHOSPHATIDYLETHANOLAMINE IS REQUIRED FOR THE PROPER TOPOLOGICAL ORGANIZATION OF THE {gamma}-AMINOBUTYRIC ACID PERMEASE (GabP) OF ESCHERICHIA COLI J. Biol. Chem., July 15, 2005; 280(28): 26032 - 26038. [Abstract] [Full Text] [PDF]

				D. A. Dodd, B. Niederoest, S. Bloechlinger, L. Dupuis, J.-P. Loeffler, and M. E. Schwab Nogo-A, -B, and -C Are Found on the Cell Surface and Interact Together in Many Different Cell Types J. Biol. Chem., April 1, 2005; 280(13): 12494 - 12502. [Abstract] [Full Text] [PDF]

				V. Llorente-Cortes and L. Badimon LDL Receptor-Related Protein and the Vascular Wall: Implications for Atherothrombosis Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 497 - 504. [Abstract] [Full Text] [PDF]

				T. P. Roosild, J. Greenwald, M. Vega, S. Castronovo, R. Riek, and S. Choe NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression Science, February 25, 2005; 307(5713): 1317 - 1321. [Abstract] [Full Text] [PDF]

				S. H. Kim, A. B. Chang, and M. H. Saier Jr Sequence similarity between multidrug resistance efflux pumps of the ABC and RND superfamilies Microbiology, August 1, 2004; 150(8): 2493 - 2495. [Full Text] [PDF]

				V. Llorente-Cortes, M. Otero-Vinas, S. Camino-Lopez, O. Llampayas, and L. Badimon Aggregated Low-Density Lipoprotein Uptake Induces Membrane Tissue Factor Procoagulant Activity and Microparticle Release in Human Vascular Smooth Muscle Cells Circulation, July 27, 2004; 110(4): 452 - 459. [Abstract] [Full Text] [PDF]

				M. Wikstrom, J. Xie, M. Bogdanov, E. Mileykovskaya, P. Heacock, A. Wieslander, and W. Dowhan Monoglucosyldiacylglycerol, a Foreign Lipid, Can Substitute for Phosphatidylethanolamine in Essential Membrane-associated Functions in Escherichia coli J. Biol. Chem., March 12, 2004; 279(11): 10484 - 10493. [Abstract] [Full Text] [PDF]