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Originally published In Press as doi:10.1074/jbc.M206136200 on November 14, 2002

J. Biol. Chem., Vol. 278, Issue 6, 3536-3544, February 7, 2003
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Expression and Characterization of Recombinant Human UDP-glucuronosyltransferases (UGTs)
UGT1A9 IS MORE RESISTANT TO DETERGENT INHIBITION THAN THE OTHER UGTs AND WAS PURIFIED AS AN ACTIVE DIMERIC ENZYME*

Mika KurkelaDagger , J. Arturo García-HorsmanDagger §, Leena LuukkanenDagger , Saila MörskyDagger , Jyrki TaskinenDagger , Marc Baumann||, Risto KostiainenDagger , Jouni HirvonenDagger , and Moshe FinelDagger **

From the Dagger  Viikki Drug Discovery Technology Center,  Department of Pharmacy, University of Helsinki, and || Protein Chemistry Unit, Institute of Biomedicine, University of Helsinki, Helsinki, Finland

Eight human liver UDP-glucuronosyltransferases (UGTs) were expressed in baculovirus-infected insect cells as fusion proteins carrying a short C-terminal extension that ends with 6 histidine residues (His tag). The activity of recombinant UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT2B4, UGT2B7, and UGT2B15 was almost fully inhibited by 0.2% Triton X-100. In the case of UGT1A9, however, glucuronidation of alpha -naphthol and scopoletin was resistant to such inhibition, whereas glucuronidation of entacapone and several other aglycones was sensitive. His-tagged UGT1A9 was purified by immobilized metal-chelating chromatography (IMAC). Purified UGT1A9 glucuronidated scopoletin at a high rate, whereas its glucuronidation activity toward entacapone was low and largely dependent on phospholipid addition. Recombinant UGT1A9 in which the His tag was replaced by hemagglutinin antigenic peptide (HA tag) was also prepared. Insect cells were co-infected with baculoviruses encoding both HA-tagged and His-tagged UGT1A9. Membranes from the co-infected cells, or a mixture of membranes from separately infected cells, were subjected to detergent extraction and IMAC, and the resulting fractions were analyzed for the presence of each type of UGT1A9 using tag-specific antibodies. In the case of separate infection, the HA-tagged UGT1A9 did not bind to the column. When co-infected with His-tagged UGT1A9, however, part of the HA-tagged enzyme was bound to the column and was eluted by imidazole concentration gradient together with the His-tagged UGT1A9, suggesting the formation of stable dimers that contain one His-tagged and one HA-tagged UGT1A9 monomers.

* This work was supported by the National Technology Agency (TEKES), Finland.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Dept. of Pharmacology and Toxicology, University of Kuopio, P. O. Box 1627, 70210 Kuopio, Finland.

** To whom correspondence should be addressed: Viikki DDTC, Dept. of Pharmacy, P. O. Box 56 (Viikinkaari 5E), 00014 University of Helsinki, Helsinki, Finland. Tel.: 358-9-191-59193; Fax: 358-9-191-59556; E-mail: moshe.finel@helsinki.fi.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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