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J. Biol. Chem., Vol. 278, Issue 6, 4184-4193, February 7, 2003
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,
From the European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany
The genomic locus SRPN10 of the
malaria vector Anopheles gambiae codes for four
alternatively spliced serine protease inhibitors of the serpin
superfamily. The four 40- to 42-kDa isoforms differ only at their C
terminus, which bears the reactive site loop, and exhibit protein
sequence similarity with other insect serpins and mammalian serpins of
the ovalbumin family. Inhibition experiments with recombinant purified
SRPN10 serpins reveal distinct and specific inhibitory activity of
three isoforms toward different proteases. All isoforms are mainly
expressed in the midgut but also in pericardial cells and hemocytes of
the mosquito. The cellular localization of SRPN10 serpins is
nucleocytoplasmic in pericardial cells, in hemocytes and in a
hemocyte-like mosquito cell line, but in the gut the proteins are
mostly localized in the nucleus. Although the transcript levels of all
SRPN10 isoforms are marginally affected by bacterial challenge, the
transcripts of two isoforms (KRAL and RCM) are induced in female
mosquitoes in response to midgut invasion by Plasmodium
berghei ookinetes. The KRAL and RCM SRPN10 isoforms represent new
potential markers to study the ookinete midgut invasion process in
anopheline mosquitoes.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ420785 (SPI21F).
Current address: Dipartimento di Biologia Evoluzionistica
Sperimentale, Università di Bologna, Via Selmi 3, Bologna 40126, Italy.
§
To whom correspondence may be addressed. Tel.: 49-6221-387-200;
Fax: 49-6221-387-211; E-mail: dg-office@embl-heidelberg.de.
¶
To whom correspondence may be addressed (current address):
Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, Vassilika Vouton, P. O. Box
1527, GR 71110, Heraklion, Greece. Tel.: 0030-2810-391149; Fax:
0030-2810-391104; E-mail: loukeris@imbb.forth.gr.
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