|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 7, 4611-4617, February 14, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The saliva of the blood-feeding insect
Rhodnius prolixus contains numerous pharmacologically
active substances. Included among these are a number of lipocalin
proteins that bind various ligands important in hemostasis and
inflammation. One such protein is a biogenic amine-binding protein
(ABP) that binds serotonin, epinephrine, and norepinephrine. Based on
amino acid alignments, it is most similar to the nitrophorin group of
lipocalins found in the same insect species. Physiologically, this
protein appears to act as both a vasodilator and platelet aggregation
inhibitor. This protein inhibits smooth muscle contraction of the rat
uterus in response to serotonin and of the rabbit aorta in response to
norepinephrine. Platelet aggregation induced by a combination of low
concentrations of ADP and either serotonin or epinephrine is inhibited
because of the binding of serotonin and epinephrine. Potentiation of
aggregation induced by low concentrations of collagen along with
serotonin or epinephrine is also inhibited. Dissociation constants for
biogenic amines were measured using isothermal titration calorimetry
and the Hummel-Dreyer method of equilibrium gel filtration. In this manner, Kd values of 102, 24, and 345 nM were found for serotonin, norepinephrine, and
epinephrine, respectively. Molecular modeling of ABP suggests that
ligand binding is mediated by interaction with the side chains of
aromatic amino acids and charged residues that line the binding pocket.
The atomic coordinates and the structure factors (code 1EUO) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).
Inhibition of Hemostasis by a High Affinity Biogenic
Amine-binding Protein from the Saliva of a Blood-feeding Insect*
§,,,
Laboratory of Malaria and Vector Research,
NIAID, National Institutes of Health and the ¶ Molecular
Interactions Resource, Division of Bioengineering and Physical Science,
Office of Research Services, Office of the Director, National
Institutes of Health, Bethesda, Maryland 20892
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Calvo, B. J. Mans, J. M. C. Ribeiro, and J. F. Andersen Multifunctionality and mechanism of ligand binding in a mosquito antiinflammatory protein PNAS, March 10, 2009; 106(10): 3728 - 3733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Mans, J. M. C. Ribeiro, and J. F. Andersen Structure, Function, and Evolution of Biogenic Amine-binding Proteins in Soft Ticks J. Biol. Chem., July 4, 2008; 283(27): 18721 - 18733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Mans, E. Calvo, J. M. C. Ribeiro, and J. F. Andersen The Crystal Structure of D7r4, a Salivary Biogenic Amine-binding Protein from the Malaria Mosquito Anopheles gambiae J. Biol. Chem., December 14, 2007; 282(50): 36626 - 36633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Calvo, F. Tokumasu, O. Marinotti, J.-L. Villeval, J. M. C. Ribeiro, and I. M. B. Francischetti Aegyptin, a Novel Mosquito Salivary Gland Protein, Specifically Binds to Collagen and Prevents Its Interaction with Platelet Glycoprotein VI, Integrin {alpha}2beta1, and von Willebrand Factor J. Biol. Chem., September 14, 2007; 282(37): 26928 - 26938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Calvo, B. J. Mans, J. F. Andersen, and J. M. C. Ribeiro Function and Evolution of a Mosquito Salivary Protein Family J. Biol. Chem., January 27, 2006; 281(4): 1935 - 1942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Gudderra, J. M. C. Ribeiro, and J. F. Andersen Structural Determinants of Factor IX(a) Binding in Nitrophorin 2, a Lipocalin Inhibitor of the Intrinsic Coagulation Pathway J. Biol. Chem., July 1, 2005; 280(26): 25022 - 25028. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |