JBC Transcription and Nuclear Factor Monoclonals

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M204652200 on December 6, 2002

J. Biol. Chem., Vol. 278, Issue 7, 4778-4785, February 14, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/7/4778    most recent
M204652200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bouschet, T.
Right arrow Articles by Journot, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bouschet, T.
Right arrow Articles by Journot, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Stimulation of the ERK Pathway by GTP-loaded Rap1 Requires the Concomitant Activation of Ras, Protein Kinase C, and Protein Kinase A in Neuronal Cells*

Tristan BouschetDagger §, Virgili PerezDagger §||, Céline FernandezDagger , Joël BockaertDagger , Alain Eychene**, and Laurent JournotDagger Dagger Dagger

From the Dagger  UPR 9023 CNRS, CCIPE-141, Rue de la Cardonille, 34094 Montpellier Cedex 05 and ** UMR 146 CNRS, Institut Curie, Bat. 110, Centre Universitaire, 91405 Orsay Cedex, France

The small GTPases Ras or Rap1 were suggested to mediate the stimulatory effect of some G protein-coupled receptors on ERK activity in neuronal cells. Accordingly, we reported here that pituitary adenylate cyclase-activating polypeptide (PACAP), whose G protein-coupled receptor triggers neuronal differentiation of the PC12 cell line via ERK1/2 activation, transiently activated Ras and induced the sustained GTP loading of Rap1. Ras mediated peak stimulation of ERK by PACAP, whereas Rap1 was necessary for the sustained activation phase. However, PACAP-induced GTP-loading of Rap1 was not sufficient to account for ERK activation by PACAP because 1) PACAP-elicited Rap1 GTP-loading depended only on phospholipase C, whereas maximal stimulation of ERK by PACAP also required the activity of protein kinase A (PKA), protein kinase C (PKC), and calcium-dependent signaling; and 2) constitutively active mutants of Rap1, Rap1A-V12, and Rap1B-V12 only minimally stimulated the ERK pathway compared with Ras-V12. The effect of Rap1A-V12 was dramatically potentiated by the concurrent activation of PKC, the cAMP pathway, and Ras, and this potentiation was blocked by dominant-negative mutants of Ras and Raf. Thus, this set of data indicated that GPCR-elicited GTP loading of Rap1 was not sufficient to stimulate efficiently ERK in PC12 cells and required the permissive co-stimulation of PKA, PKC, or Ras.

* This work was supported in part by grants from the Centre National de la Recherche Scientifique and Grant CT-1999-00602 from the European Commission (to L. J.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

Recipient of a pre-doctoral fellowship from the Ministère de la Recherche, the Fondation pour la Recherche Médicale, and the Association pour la Recherche Contre le Cancer.

|| Recipient of post-doctoral fellowships from the Fondation pour la Recherche Médicale, the Association pour la Recherche Contre le Cancer, and the Spanish Ministerio de Educacion, Cultura y Deporte.

Dagger Dagger To whom correspondence should be addressed. Tel.: 33-467-142932; Fax: 33-467-542432; E-mail: journot@montp.inserm.fr.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
A. Ravni, D. Vaudry, M. J. Gerdin, M. V. Eiden, A. Falluel-Morel, B. J. Gonzalez, H. Vaudry, and L. E. Eiden
A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Mol. Pharmacol., June 1, 2008; 73(6): 1688 - 1708.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. P. Ford, K. V. Wong, V. B. Lu, E. Posse de Chaves, and P. A. Smith
Differential Neurotrophic Regulation of Sodium and Calcium Channels in an Adult Sympathetic Neuron
J Neurophysiol, March 1, 2008; 99(3): 1319 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X.-L. Meng, O. M. Rennert, and W.-Y. Chan
Human Chorionic Gonadotropin Induces Neuronal Differentiation of PC12 Cells through Activation of Stably Expressed Lutropin/Choriogonadotropin Receptor
Endocrinology, December 1, 2007; 148(12): 5865 - 5873.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
P. Sun, H. Watanabe, K. Takano, T. Yokoyama, J.-i. Fujisawa, and T. Endo
Sustained activation of M-Ras induced by nerve growth factor is essential for neuronal differentiation of PC12 cells.
Genes Cells, September 1, 2006; 11(9): 1097 - 1113.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. B. Arthur, K. Akassoglou, and P. A. Insel
P2Y2 receptor activates nerve growth factor/TrkA signaling to enhance neuronal differentiation
PNAS, December 27, 2005; 102(52): 19138 - 19143.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Kiermayer, R. M. Biondi, J. Imig, G. Plotz, J. Haupenthal, S. Zeuzem, and A. Piiper
Epac Activation Converts cAMP from a Proliferative into a Differentiation Signal in PC12 Cells
Mol. Biol. Cell, December 1, 2005; 16(12): 5639 - 5648.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Romano, K. Magalon, A. Ciampini, C. Talet, A. Enjalbert, and C. Gerard
Differential Involvement of the Ras and Rap1 Small GTPases in Vasoactive Intestinal and Pituitary Adenylyl Cyclase Activating Polypeptides Control of the Prolactin Gene
J. Biol. Chem., December 19, 2003; 278(51): 51386 - 51394.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Gao, E. Nikulina, W. Mellado, and M. T. Filbin
Neurotrophins Elevate cAMP to Reach a Threshold Required to Overcome Inhibition by MAG through Extracellular Signal-Regulated Kinase-Dependent Inhibition of Phosphodiesterase
J. Neurosci., December 17, 2003; 23(37): 11770 - 11777.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Lahlou, N. Saint-Laurent, J.-P. Esteve, A. Eychene, L. Pradayrol, S. Pyronnet, and C. Susini
sst2 Somatostatin Receptor Inhibits Cell Proliferation through Ras-, Rap1-, and B-Raf-dependent ERK2 Activation
J. Biol. Chem., October 10, 2003; 278(41): 39356 - 39371.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.