|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 7, 5009-5020, February 14, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of Molecular, Cellular and Developmental
Biology and Biological Chemistry, University of Michigan and the
Life Sciences Institute, Ann Arbor, Michigan 48109
Autophagy, pexophagy, and the Cvt pathway are
processes that deliver hydrolytic enzymes and substrates to the yeast
vacuole/lysosome via double-membrane cytosolic vesicles. Whereas these
pathways operate under different nutritional conditions, they all
employ common machinery with only a few specific factors assisting in the choice of the delivery program and the membrane source for the
sequestering vesicle. We found that the YKR020w gene
product is essential for Cvt vesicle formation but not for pexophagy or induction of autophagy. Autophagosomes in the ykr020w
Vps51 Is Part of the Yeast Vps Fifty-three Tethering
Complex Essential for Retrograde Traffic from the Early Endosome
and Cvt Vesicle Completion*
mutant, however, have a reduced size. We demonstrate that Ykr020 is a subunit of the Vps fifty-three tethering complex, composed of Vps52,
Vps53, and Vps54, which is required for retrograde traffic from the
early endosome back to the late Golgi, and for this reason we named it
Vps51. This complex participates in a fusion event together with Tlg1
and Tlg2, two SNAREs also shown to be necessary for Cvt vesicle
assembly. In particular, those factors are essential to correctly
target the prApe1-Cvt19-Cvt9 complex to the preautophagosomal structure, the site of Cvt vesicle formation.
*
This work was supported by National Institutes of Health
Public Health Service Grant GM53396 (to D. J. K.) and by a
European Molecular Biology Organization long-term fellowship (to
F. R.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: University of
Michigan, Dept. of Molecular, Cellular and Developmental Biology, Ann
Arbor, MI 48109-1048. Tel.: 734-615-6556; Fax: 734-647-0884; E-mail:
klionsky@umich.edu.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. J. Curwin, G. D. Fairn, and C. R. McMaster Phospholipid Transfer Protein Sec14 Is Required for Trafficking from Endosomes and Regulates Distinct trans-Golgi Export Pathways J. Biol. Chem., March 13, 2009; 284(11): 7364 - 7375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Krick, Y. Muehe, T. Prick, S. Bremer, P. Schlotterhose, E.-L. Eskelinen, J. Millen, D. S. Goldfarb, and M. Thumm Piecemeal Microautophagy of the Nucleus Requires the Core Macroautophagy Genes Mol. Biol. Cell, October 1, 2008; 19(10): 4492 - 4505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Perez-Victoria, G. A. Mardones, and J. S. Bonifacino Requirement of the Human GARP Complex for Mannose 6-phosphate-receptor-dependent Sorting of Cathepsin D to Lysosomes Mol. Biol. Cell, June 1, 2008; 19(6): 2350 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kama, M. Robinson, and J. E. Gerst Btn2, a Hook1 Ortholog and Potential Batten Disease-Related Protein, Mediates Late Endosome-Golgi Protein Sorting in Yeast Mol. Cell. Biol., January 15, 2007; 27(2): 605 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Reggiori and D. J. Klionsky Atg9 sorting from mitochondria is impaired in early secretion and VFT-complex mutants in Saccharomyces cerevisiae J. Cell Sci., July 15, 2006; 119(14): 2903 - 2911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Quenneville, T.-Y. Chao, J. M. McCaffery, and E. Conibear Domains within the GARP Subunit Vps54 Confer Separate Functions in Complex Assembly and Early Endosome Recognition Mol. Biol. Cell, April 1, 2006; 17(4): 1859 - 1870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Wagner, E. E. Molnar, B. A. Molitoris, and M. G. Goebl Loss of the Homotypic Fusion and Vacuole Protein Sorting or Golgi-Associated Retrograde Protein Vesicle Tethering Complexes Results in Gentamicin Sensitivity in the Yeast Saccharomyces cerevisiae Antimicrob. Agents Chemother., February 1, 2006; 50(2): 587 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Reggiori, I. Monastyrska, T. Shintani, and D. J. Klionsky The Actin Cytoskeleton Is Required for Selective Types of Autophagy, but Not Nonspecific Autophagy, in the Yeast Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2005; 16(12): 5843 - 5856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Efe, F. Plattner, N. Hulo, D. Kressler, S. D. Emr, and O. Deloche Yeast Mon2p is a highly conserved protein that functions in the cytoplasm-to-vacuole transport pathway and is required for Golgi homeostasis J. Cell Sci., October 15, 2005; 118(20): 4751 - 4764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oka and M. Krieger Multi-Component Protein Complexes and Golgi Membrane Trafficking J. Biochem., February 1, 2005; 137(2): 109 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Meiling-Wesse, H. Barth, C. Voss, E.-L. Eskelinen, U. D. Epple, and M. Thumm Atg21 Is Required for Effective Recruitment of Atg8 to the Preautophagosomal Structure during the Cvt Pathway J. Biol. Chem., September 3, 2004; 279(36): 37741 - 37750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Stromhaug, F. Reggiori, J. Guan, C.-W. Wang, and D. J. Klionsky Atg21 Is a Phosphoinositide Binding Protein Required for Efficient Lipidation and Localization of Atg8 during Uptake of Aminopeptidase I by Selective Autophagy Mol. Biol. Cell, August 1, 2004; 15(8): 3553 - 3566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sipos, J. H. Brickner, E.J. Brace, L. Chen, A. Rambourg, F. Kepes, and R. S. Fuller Soi3p/Rav1p Functions at the Early Endosome to Regulate Endocytic Trafficking to the Vacuole and Localization of Trans-Golgi Network Transmembrane Proteins Mol. Biol. Cell, July 1, 2004; 15(7): 3196 - 3209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lobstein, A. Guyon, M. Ferault, D. Twell, G. Pelletier, and S. Bonhomme The Putative Arabidopsis Homolog of Yeast Vps52p Is Required for Pollen Tube Elongation, Localizes to Golgi, and Might Be Involved in Vesicle Trafficking Plant Physiology, July 1, 2004; 135(3): 1480 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. V. Budovskaya, J. S. Stephan, F. Reggiori, D. J. Klionsky, and P. K. Herman The Ras/cAMP-dependent Protein Kinase Signaling Pathway Regulates an Early Step of the Autophagy Process in Saccharomyces cerevisiae J. Biol. Chem., May 14, 2004; 279(20): 20663 - 20671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Reggiori, C.-W. Wang, U. Nair, T. Shintani, H. Abeliovich, and D. J. Klionsky Early Stages of the Secretory Pathway, but Not Endosomes, Are Required for Cvt Vesicle and Autophagosome Assembly in Saccharomyces cerevisiae Mol. Biol. Cell, May 1, 2004; 15(5): 2189 - 2204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-W. Wang, P. E. Stromhaug, E. J. Kauffman, L. S. Weisman, and D. J. Klionsky Yeast homotypic vacuole fusion requires the Ccz1-Mon1 complex during the tethering/docking stage J. Cell Biol., December 8, 2003; 163(5): 973 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Tucker, F. Reggiori, W. A. Dunn Jr., and D. J. Klionsky Atg23 Is Essential for the Cytoplasm to Vacuole Targeting Pathway and Efficient Autophagy but Not Pexophagy J. Biol. Chem., November 28, 2003; 278(48): 48445 - 48452. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |