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J. Biol. Chem., Vol. 278, Issue 7, 5235-5241, February 14, 2003
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From the Molecular Biophysics Unit, Indian Institute of Science,
Bangalore-560012, India
Some members of the DNA-binding protein from
stationary phase cells (Dps) family of proteins have been shown to play
an important role in protecting microorganisms from oxidative or
nutritional stress. Dps homologs have been identified in various
bacteria such as Escherichia coli, Bacillus
subtilis, and Listeria innocua. Recently we have
reported the presence of a Dps homolog, Ms-Dps, in Mycobacterium
smegmatis. Ms-Dps was found to have a nonspecific DNA binding
ability. Here we have detected two stable oligomeric forms of Ms-Dps
in vitro, a trimeric and a dodecameric form. Interestingly, the conversion of Dps from a trimeric to a dodecameric form takes place
upon incubation at 37 °C for 12 h. These two oligomeric forms
differ in their DNA binding properties. The dodecameric form is capable
of DNA binding and forming large crystalline arrays with DNA, whereas
the trimeric form cannot do so. However, even in the absence of DNA
binding, the trimeric form has the capacity to protect the DNA against
Fenton's-mediated damage. The protection is afforded by the
ferroxidase activity of the trimer. However, the trimeric form cannot
protect DNA from DNaseI attack, for which a direct physical shielding
of DNA by the dodecamer is required. Thus we suggest that Ms-Dps
provides a bimodal protection of DNA by its two different oligomeric forms.
Bimodal Protection of DNA by Mycobacterium
smegmatis DNA-binding Protein from Stationary Phase
Cells*
and
*
This work was supported by the Department of Biotechnology
of the government of India.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of Council of Scientific and Industrial Research fellowship.
§
To whom correspondence should be addressed. Tel.: 91-80-3942836;
Fax: 91-80-3600535; E-mail: dipankar@mbu.iisc.ernet.in.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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