JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208132200 on December 16, 2002

J. Biol. Chem., Vol. 278, Issue 8, 5828-5836, February 21, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/8/5828    most recent
M208132200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dello Russo, C.
Right arrow Articles by Feinstein, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dello Russo, C.
Right arrow Articles by Feinstein, D. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Peroxisome Proliferator-activated Receptor gamma  Thiazolidinedione Agonists Increase Glucose Metabolism in Astrocytes*

Cinzia Dello RussoDagger §, Vitaliy GavrilyukDagger , Guy WeinbergDagger ||, Angeles Almeida**, Juan P. Bolanos**, June PalmerDagger , Dale PelligrinoDagger , Elena GaleaDagger , and Douglas L. FeinsteinDagger ||

From the Dagger  Department of Anesthesiology, University of Illinois, Chicago, Illinois, 60612, || Veterans Affairs Chicago Health Care System West Side Division, Chicago, Illinois, 60680, ** Departmento de Bioquimica y Biologia Molecular, Universidad de Salamanca, Salamanca 37007, Spain, and § Institute of Pharmacology, Catholic University Medical School, Rome 00168, Italy

Activation of peroxisome proliferator-activated receptors (PPARs) can regulate brain physiology and provide protection in models of neurological disease; however, neither their exact targets nor mechanisms of action in brain are known. In many cells, PPARgamma agonists increase glucose uptake and metabolism. Because astrocytes store glucose and provide lactate to neurons on demand, we tested effects of PPARgamma agonists on astroglial glucose metabolism. Incubation of cortical astrocytes with the PPARgamma thiazolidinedione (TZD) agonist pioglitazone (Pio) significantly increased glucose consumption in a time- and dose-dependent manner, with maximal increase of 36% observed after 4 h in 30 µM Pio. Pio increased 2-deoxy-glucose uptake because of increased flux through the type 1 glucose transporter. However, at this time point Pio did not increase type 1 glucose transporter expression, nor were its effects blocked by transcriptional or translational inhibitors. Pio also increased astrocyte lactate production as soon as 3 h after incubation. These effects were replicated by other TZDs; however, the order of efficacy (troglitazone > pioglitazone > rosiglitazone) suggests that effects were not mediated via PPARgamma activation. TZDs increased astrocyte cAMP levels, and their glucose modifying effects were reduced by protein kinase A inhibitors. TZDs inhibited state III respiration in isolated brain mitochondria, whereas in astrocytes they caused mitochondrial membrane hyperpolarization. Pio protected astrocytes against hypoglycemia-induced cell death. Finally, glucose uptake was modified in brain sections prepared from Pio-fed rats. These results demonstrate that TZDs modify astrocyte metabolism and mitochondrial function, which could be beneficial in neurological conditions where glucose availability is reduced.

* This work was supported in part by grants from the National Multiple Sclerosis Society, Takeda Pharmaceuticals North America, and GlaxoSmithKline.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: 1819 West Polk St., MC519, Rm. 540, Chicago, IL 60612. Tel.: 312-996-8377; Fax: 312-996-9680; E-mail: cdr@uic.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Therapeutic Advances in Cardiovascular DiseaseHome page
M. Collino, N. S.A. Patel, and C. Thiemermann
Review: PPARs as new therapeutic targets for the treatment of cerebral ischemia/reperfusion injury
Therapeutic Advances in Cardiovascular Disease, June 1, 2008; 2(3): 179 - 197.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
M. Miksa, R. Wu, X. Cui, W. Dong, P. Das, H. H. Simms, T. S. Ravikumar, and P. Wang
Vasoactive Hormone Adrenomedullin and Its Binding Protein: Anti-Inflammatory Effects by Up-Regulating Peroxisome Proliferator-Activated Receptor-{gamma}
J. Immunol., November 1, 2007; 179(9): 6263 - 6272.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
K. Y. Kim, J. H. Ahn, and H. G. Cheon
Apoptotic Action of Peroxisome Proliferator-Activated Receptor-{gamma} Activation in Human Non Small-Cell Lung Cancer Is Mediated via Proline Oxidase-Induced Reactive Oxygen Species Formation
Mol. Pharmacol., September 1, 2007; 72(3): 674 - 685.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. Soller, S. Drose, U. Brandt, B. Brune, and A. von Knethen
Mechanism of Thiazolidinedione-Dependent Cell Death in Jurkat T Cells
Mol. Pharmacol., June 1, 2007; 71(6): 1535 - 1544.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S.-W. Park, J.-H. Yi, G. Miranpuri, I. Satriotomo, K. Bowen, D. K. Resnick, and R. Vemuganti
Thiazolidinedione Class of Peroxisome Proliferator-Activated Receptor {gamma} Agonists Prevents Neuronal Damage, Motor Dysfunction, Myelin Loss, Neuropathic Pain, and Inflammation after Spinal Cord Injury in Adult Rats
J. Pharmacol. Exp. Ther., March 1, 2007; 320(3): 1002 - 1012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F. Turturro, R. Oliver III, E. Friday, I. Nissim, and T. Welbourne
Troglitazone and pioglitazone interactions via PPAR-{gamma}-independent and -dependent pathways in regulating physiological responses in renal tubule-derived cell lines
Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1137 - C1146.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
D. Safiulina, N. Peet, E. Seppet, A. Zharkovsky, and A. Kaasik
Dehydroepiandrosterone Inhibits Complex I of the Mitochondrial Respiratory Chain and is Neurotoxic In Vitro and In Vivo at High Concentrations
Toxicol. Sci., October 1, 2006; 93(2): 348 - 356.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. H. Muzumdar, X. Ma, S. Fishman, X. Yang, G. Atzmon, P. Vuguin, F. H. Einstein, D. Hwang, P. Cohen, and N. Barzilai
Central and Opposing Effects of IGF-I and IGF-Binding Protein-3 on Systemic Insulin Action.
Diabetes, October 1, 2006; 55(10): 2788 - 2796.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
F. Pifferi, F. Roux, B. Langelier, J.-M. Alessandri, S. Vancassel, M. Jouin, M. Lavialle, and P. Guesnet
(n-3) Polyunsaturated Fatty Acid Deficiency Reduces the Expression of Both Isoforms of the Brain Glucose Transporter GLUT1 in Rats
J. Nutr., September 1, 2005; 135(9): 2241 - 2246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H.-L. Xu, V. Gavrilyuk, H. M. Wolde, V. L. Baughman, and D. A. Pelligrino
Regulation of rat pial arteriolar smooth muscle relaxation in vivo through multidrug resistance protein 5-mediated cGMP efflux
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H2020 - H2027.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
B. Brunmair, K. Staniek, F. Gras, N. Scharf, A. Althaym, R. Clara, M. Roden, E. Gnaiger, H. Nohl, W. Waldhausl, et al.
Thiazolidinediones, Like Metformin, Inhibit Respiratory Complex I: A Common Mechanism Contributing to Their Antidiabetic Actions?
Diabetes, April 1, 2004; 53(4): 1052 - 1059.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Perez-Ortiz, P. Tranque, C. F. Vaquero, B. Domingo, F. Molina, S. Calvo, J. Jordan, V. Cena, and J. Llopis
Glitazones Differentially Regulate Primary Astrocyte and Glioma Cell Survival: INVOLVEMENT OF REACTIVE OXYGEN SPECIES AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-{gamma}
J. Biol. Chem., March 5, 2004; 279(10): 8976 - 8985.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.