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J. Biol. Chem., Vol. 278, Issue 8, 5902-5911, February 21, 2003
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From the National Food Research Institute, Tsukuba,
Ibaraki 305-8642, Japan
The 52-kDa protein, EshA, whose expression is
controlled developmentally, is produced during the late growth phase of
Streptomyces spp. We found that disruption of the
eshA gene, which encodes the EshA protein, abolishes the
aerial mycelium formation and streptomycin production in
Streptomyces griseus when grown on an agar plate. The
eshA disruptant KO-390 demonstrated a reduced amount of
expression of the transcriptional activator strR, thus accounting for the failure to produce streptomycin. KO-390 was found to
accumulate deoxynucleoside triphosphates at high levels, including
dGTP, at late growth phase. The accumulation of dGTP was a cause for
the impaired ability of KO-390 to produce aerial mycelium, because the
ability to form aerial mycelium was completely repaired by addition of
decoyinine, an inhibitor of GMP synthetase. The accumulation of
dNTP in KO-390 coincided with a reduced rate of DNA synthesis. The
developmental time frame of these phenomena in KO-390 matched a burst
of EshA expression in the wild-type strain. In contrast to
S. griseus, the eshA disruption did
not affect the ability for Streptomyces coelicolor to form
aerial mycelium and did not result in the aberrant accumulation of dNTP accompanied by arrest of DNA synthesis, implying qualitative
differences in addition to quantitative differences between the two
EshA proteins. We propose that the S. griseus
EshA protein somehow positively affects (or regulates) the replication
of DNA in wild-type cells at late growth phase but leads to aberrant
phenotypes in mutant cells due to the disturbed DNA replication. The
EshA protein was found to exist as a multimer (~20-mers) creating a
cubic-like structure with a diameter of 27 nm and located predominantly
in cytoplasm.
Genetic and Biochemical Characterization of EshA, a Protein That
Forms Large Multimers and Affects Developmental Processes in
Streptomyces griseus*
,
*
This work was supported in part by a grant from the
Organized Research Combination System of the Science and Technology
Agency of Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Translational Research Department, Mitsubishi
Kagaku Institute of Life Sciences, Yokohama Research Center, 1000, Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa 227-8502, Japan.
§
Present address: Dept. of Biological Science, Graduate School of
Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuda-Cho, Midori-ku, Yokohama 226-8501, Japan.
¶
Present address: Dept. of Microbiology, School of
Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi,
Chiba 274-8501, Japan.
To whom correspondence should be addressed: National Food
Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Tel.: 81-29-838-8125; Fax: 81-29-838-7996; E-mail:
kochi@affrc.go.jp.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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