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J. Biol. Chem., Vol. 278, Issue 8, 5902-5911, February 21, 2003
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,
From the National Food Research Institute, Tsukuba,
Ibaraki 305-8642, Japan
The 52-kDa protein, EshA, whose expression is
controlled developmentally, is produced during the late growth phase of
Streptomyces spp. We found that disruption of the
eshA gene, which encodes the EshA protein, abolishes the
aerial mycelium formation and streptomycin production in
Streptomyces griseus when grown on an agar plate. The
eshA disruptant KO-390 demonstrated a reduced amount of
expression of the transcriptional activator strR, thus accounting for the failure to produce streptomycin. KO-390 was found to
accumulate deoxynucleoside triphosphates at high levels, including
dGTP, at late growth phase. The accumulation of dGTP was a cause for
the impaired ability of KO-390 to produce aerial mycelium, because the
ability to form aerial mycelium was completely repaired by addition of
decoyinine, an inhibitor of GMP synthetase. The accumulation of
dNTP in KO-390 coincided with a reduced rate of DNA synthesis. The
developmental time frame of these phenomena in KO-390 matched a burst
of EshA expression in the wild-type strain. In contrast to
S. griseus, the eshA disruption did
not affect the ability for Streptomyces coelicolor to form
aerial mycelium and did not result in the aberrant accumulation of dNTP accompanied by arrest of DNA synthesis, implying qualitative
differences in addition to quantitative differences between the two
EshA proteins. We propose that the S. griseus
EshA protein somehow positively affects (or regulates) the replication
of DNA in wild-type cells at late growth phase but leads to aberrant
phenotypes in mutant cells due to the disturbed DNA replication. The
EshA protein was found to exist as a multimer (~20-mers) creating a
cubic-like structure with a diameter of 27 nm and located predominantly
in cytoplasm.
Present address: Translational Research Department, Mitsubishi
Kagaku Institute of Life Sciences, Yokohama Research Center, 1000, Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa 227-8502, Japan.
§
Present address: Dept. of Biological Science, Graduate School of
Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuda-Cho, Midori-ku, Yokohama 226-8501, Japan.
¶
Present address: Dept. of Microbiology, School of
Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi,
Chiba 274-8501, Japan.
To whom correspondence should be addressed: National Food
Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Tel.: 81-29-838-8125; Fax: 81-29-838-7996; E-mail:
kochi@affrc.go.jp.
This article has been cited by other articles:
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  N. Saito, J. Xu, T. Hosaka, S. Okamoto, H. Aoki, M. J. Bibb, and K. Ochi EshA Accentuates ppGpp Accumulation and Is Conditionally Required for Antibiotic Production in Streptomyces coelicolor A3(2) J. Bacteriol., July 1, 2006; 188(13): 4952 - 4961. [Abstract] [Full Text] [PDF]
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 D.-W. Kim, K. F. Chater, K.-J. Lee, and A. Hesketh Effects of growth phase and the developmentally significant bldA-specified tRNA on the membrane-associated proteome of Streptomyces coelicolor Microbiology, August 1, 2005; 151(8): 2707 - 2720. [Abstract] [Full Text] [PDF]
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 K. Takahashi, K. Kasai, and K. Ochi Identification of the bacterial alarmone guanosine 5'-diphosphate 3'-diphosphate (ppGpp) in plants PNAS, March 23, 2004; 101(12): 4320 - 4324. [Abstract] [Full Text] [PDF]
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