Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208288200 on December 17, 2002

J. Biol. Chem., Vol. 278, Issue 8, 6018-6026, February 21, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/8/6018    most recent
M208288200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujimori, K.
Right arrow Articles by Urade, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujimori, K.
Right arrow Articles by Urade, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulation of Lipocalin-type Prostaglandin D Synthase Gene Expression by Hes-1 through E-box and Interleukin-1beta via Two NF-kappa B Elements in Rat Leptomeningeal Cells*

Ko FujimoriDagger §, Yasushi FujitaniDagger , Keiichi KadoyamaDagger , Haruko KumanogohDagger , Koichi Ishikawa||, and Yoshihiro UradeDagger **

From the Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation and the Dagger  Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874 and the || Institute for Molecular and Cellular Regulation, Gunma University, Showa-machi, Maebashi, Gunma 371-8512, Japan

The promoter function of the rat lipocalin-type prostaglandin D synthase (L-PGDS) gene was characterized in primary cultures of leptomeningeal cells prepared from the neonatal rat brain. Luciferase reporter assays with deletion and site-directed mutation of the promoter region (-1250 to +77) showed that an AP-2 element at -109 was required for activation and an E-box at +57, for repression. Binding of nuclear factors to each of these cis-elements was demonstrated by an electrophoretic mobility shift assay. Several components of the Notch-Hes signaling pathway, Jagged, Notch1, Notch3, and Hes-1, were expressed in the leptomeningeal cells. Human Hes-1 co-expressed in the leptomeningeal cells bound to the E-box of the rat L-PGDS gene, and repressed the promoter activity of the rat L-PGDS gene in a dose-dependent manner. The L-PGDS gene expression was up-regulated slowly by interleukin-1beta to the maximum level at 24 h. The reporter assay with deletion and mutation revealed that two NF-kappa B elements at -1106 and -291 were essential for this up-regulation. Binding of two NF-kappa B subunits, p65 and c-Rel, to these two NF-kappa B elements occurred after the interleukin-1beta treatment. Therefore, the L-PGDS gene is the first gene identified as the target for the Notch-Hes signal through the E-box among a variety of genes involved in the prostanoid biosynthesis, classified to the lipocalin family, and expressed in the leptomeninges. Moreover, the L-PGDS gene is a unique gene that is activated slowly by the NF-kappa B system.

* This work was supported in part by a grant from the program for Core Research for Evolutional Science and Technology of Japan Science Technology Corporation (to Y. U.), Grant-in-aid for Scientific Research 12558078 from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to Y. U.), grants from the Ground Research Announcement for Space Utilization Promotion from the Japan Space Forum (to Y. U.), the Pilot Applied Research Project for the Industrial Use of Space from the National Space Development Agency of Japan (NASDA) and Japan Space Utilization Promotion Center (JSUP) (to Y. U.), the Takeda Science Foundation (to K. F., Y. F., H. K., and Y. U.), the Special Coordination Fund for Promoting Science and Technology (to Y. U.), and Osaka City.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 1-98 Kasugadenaka 3-chome, Konohana-ku, Osaka 554-8558, Japan.

Present address: Pharmaceutical Research Division, Takeda Chemical Industries, Ltd., 17-85, Jusohonmachi 2-chome, Yodogawa-ku, Osaka 532-8686, Japan.

** To whom correspondence should be addressed. Tel.: 81-6-6872-4851; Fax: 81-6-6872-2841; E-mail: uradey@obi.or.jp.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
S. Delbosc, M. Glorian, A.-S. Le Port, G. Bereziat, M. Andreani, and I. Limon
The Benefit of Docosahexanoic Acid on the Migration of Vascular Smooth Muscle Cells Is Partially Dependent on Notch Regulation of MMP-2/-9
Am. J. Pathol., May 1, 2008; 172(5): 1430 - 1440.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Clement, M. Gueguen, M. Glorian, R. Blaise, M. Andreani, C. Brou, P. Bausero, and I. Limon
Notch3 and IL-1beta exert opposing effects on a vascular smooth muscle cell inflammatory pathway in which NF-{kappa}B drives crosstalk
J. Cell Sci., October 1, 2007; 120(19): 3352 - 3361.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Fischer and M. Gessler
Delta Notch and then? Protein interactions and proposed modes of repression by Hes and Hey bHLH factors
Nucleic Acids Res., July 14, 2007; 35(14): 4583 - 4596.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Fujimori, K. Aritake, and Y. Urade
A Novel Pathway to Enhance Adipocyte Differentiation of 3T3-L1 Cells by Up-regulation of Lipocalin-type Prostaglandin D Synthase Mediated by Liver X Receptor-activated Sterol Regulatory Element-binding Protein-1c
J. Biol. Chem., June 22, 2007; 282(25): 18458 - 18466.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wilhelm, R. Hiramatsu, H. Mizusaki, L. Widjaja, A. N. Combes, Y. Kanai, and P. Koopman
SOX9 Regulates Prostaglandin D Synthase Gene Transcription in Vivo to Ensure Testis Development
J. Biol. Chem., April 6, 2007; 282(14): 10553 - 10560.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. A. Ross, S. Hannenhalli, J. W. Tobias, N. Cooch, R. Shiekhattar, and T. Kadesch
Functional Analysis of Hes-1 in Preadipocytes
Mol. Endocrinol., March 1, 2006; 20(3): 698 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Mouihate, S. Ellis, E.-M. Harre, and Q. J. Pittman
Fever suppression in near-term pregnant rats is dissociated from LPS-activated signaling pathways
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1265 - R1272.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. Martinez-Florez, B. Gutierrez-Fernandez, S. Sanchez-Campos, J. Gonzalez-Gallego, and M. J. Tunon
Quercetin Attenuates Nuclear Factor-{kappa}B Activation and Nitric Oxide Production in Interleukin-1{beta}-Activated Rat Hepatocytes
J. Nutr., June 1, 2005; 135(6): 1359 - 1365.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Fujimori, K. Kadoyama, and Y. Urade
Protein Kinase C Activates Human Lipocalin-type Prostaglandin D Synthase Gene Expression through De-repression of Notch-HES Signaling and Enhancement of AP-2{beta} Function in Brain-derived TE671 Cells
J. Biol. Chem., May 6, 2005; 280(18): 18452 - 18461.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Aguilera, R. Hoya-Arias, G. Haegeman, L. Espinosa, and A. Bigas
Recruitment of I{kappa}B{alpha} to the hes1 promoter is associated with transcriptional repression
PNAS, November 23, 2004; 101(47): 16537 - 16542.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. A. Altar, P. Laeng, L. W. Jurata, J. A. Brockman, A. Lemire, J. Bullard, Y. V. Bukhman, T. A. Young, V. Charles, and M. G. Palfreyman
Electroconvulsive Seizures Regulate Gene Expression of Distinct Neurotrophic Signaling Pathways
J. Neurosci., March 17, 2004; 24(11): 2667 - 2677.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement